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GRK4 (NM_001004057) Human Tagged ORF Clone
CAT＃: RG215579
- TrueORF®
GRK4 (tGFP-tagged) - Human G protein-coupled receptor kinase 4 (GRK4), transcript variant 3
ORF Plasmid: DDK
"NM_001004057" in other vectors (6)
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CNY 5,130.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GPRK2L; GPRK4; GRK4a; IT11 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215579 representing NM_001004057
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTCGAGAACATCGTGGCCAACTCGCTGCTGCTGAAAGCGCGTCAAGGAGGATATGGCAAAAAAA GTGGTCGTAGTAAAAAATGGAAGGAGATACTGACACTGCCTCCTGTCAGCCAGTGCAGTGAGCTTAGACA TTCCATTGAAAAGGATTATAGCAGTCTTTGTGACAAGCAACCGATAGGAAGACGTCTCTTCAGGCAGTTC TGTGATACCAAACCCACTCTAAAGAGGCACATTGAATTCTTGGATGCAGTGGCAGAATATGAAGTTGCCG ATGATGAGGACCGAAGTGATTGTGGACTGTCAATCTTAGATAGATTCTTCAATGATAAGTTGGCAGCCCC TTTACCAGAAATACCTCCAGATGTTGTGACAGAATGTAGATTGGGACTGAAGGAGGAGAACCCTTCCAAA AAAGCCTTTGAGGAATGTACTAGAGTTGCCCATAACTACCTAAGAGGGGAACCATTTGAAGAATACCAAG AAAGCTCATATTTTTCTCAGTTTTTACAATGGAAATGGCTGGAAAGGCAACCCGTAACAAAGAACACATT TAGACATTACAGAGTTCTAGGAAAAGGCGGATTTGGAGAGGTTTGCGCCTGTCAAGTGCGAGCCACAGGA AAAATGTATGCCTGCAAAAAGCTACAAAAAAAAAGAATAAAGAAGAGGAAAGGTGAAGCTATGGCTCTAA ATGAGAAAAGAATTCTGGAGAAAGTGCAAAGTAGATTCGTAGTTAGTTTAGCCTACGCTTATGAAACCAA AGATGCCTTGTGCTTGGTGCTCACCATTATGAATGGAGGGGATTTGAAGTTTCACATTTACAACCTGGGC AATCCCGGCTTTGATGAGCAGAGAGCCGTTTTCTATGCTGCAGAGCTGTGTTGCGGCTTGGAAGATTTAC AGAGGGAAAGAATTGTATACAGAGACTTGAAGCCTGAGAATATTCTCCTTGATGATCGTGGACACATCCG GATTTCAGACCTCGGTTTGGCCACAGAGATCCCAGAAGGACAGAGGGTTCGAGGAAGAGTTGGAACAGTC GGCTACATGGCACCTGAAGTTGTCAATAATGAAAAGTATACGTTTAGTCCCGATTGGTGGGGACTTGGCT GTCTGATCTATGAAATGATTCAGGGACATTCTCCATTCAAAAAATACAAAGAGAAAGTCAAATGGGAGGA GGTCGATCAAAGAATCAAGAATGATACCGAGGAGTATTCTGAGAAGTTTTCAGAGGATGCCAAATCTATC TGCAGGATGTTACTCACCAAGAATCCAAGCAAGCGGCTGGGCTGCAGGGGCGAGGGAGCGGCTGGGGTGA AGCAGCACCCCGTGTTCAAGGACATCAACTTCAGGAGGCTGGAGGCAAACATGCTGGAGCCCCCTTTCTG TCCTGATCCTCATGCCGTTTACTGTAAGGACGTCCTGGATATCGAGCAGTTCTCGGTGGTGAAAGGGATC TACCTGGACACCGCAGATGAAGACTTCTATGCTCGGTTTGCTACCGGGTGTGTCTCCATCCCCTGGCAGA ATGAGGGCTGCCTGACCATGGTCCCCAGTGAGAAGGAAGTGGAACCCAAGCAATGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215579 representing NM_001004057
Red=Cloning site Green=Tags(s) MELENIVANSLLLKARQGGYGKKSGRSKKWKEILTLPPVSQCSELRHSIEKDYSSLCDKQPIGRRLFRQF CDTKPTLKRHIEFLDAVAEYEVADDEDRSDCGLSILDRFFNDKLAAPLPEIPPDVVTECRLGLKEENPSK KAFEECTRVAHNYLRGEPFEEYQESSYFSQFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATG KMYACKKLQKKRIKKRKGEAMALNEKRILEKVQSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNLG NPGFDEQRAVFYAAELCCGLEDLQRERIVYRDLKPENILLDDRGHIRISDLGLATEIPEGQRVRGRVGTV GYMAPEVVNNEKYTFSPDWWGLGCLIYEMIQGHSPFKKYKEKVKWEEVDQRIKNDTEEYSEKFSEDAKSI CRMLLTKNPSKRLGCRGEGAAGVKQHPVFKDINFRRLEANMLEPPFCPDPHAVYCKDVLDIEQFSVVKGI YLDTADEDFYARFATGCVSIPWQNEGCLTMVPSEKEVEPKQC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001004057 |
| ORF Size | 1596 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001004057.2 |
| RefSeq Size | 2183 bp |
| RefSeq ORF | 1599 bp |
| Locus ID | 2868 |
| UniProt ID | P32298 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Chemokine signaling pathway, Endocytosis |
| Gene Summary | This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215579 | GRK4 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase 4 (GRK4), transcript variant 3 |
CNY 3,960.00 |
|
| RC215579L1 | Lenti ORF clone of Human G protein-coupled receptor kinase 4 (GRK4), transcript variant 3, Myc-DDK-tagged |
CNY 6,360.00 |
|
| RC215579L2 | Lenti ORF clone of Human G protein-coupled receptor kinase 4 (GRK4), transcript variant 3, mGFP tagged |
CNY 6,560.00 |
|
| RC215579L3 | Lenti ORF clone of Human G protein-coupled receptor kinase 4 (GRK4), transcript variant 3, Myc-DDK-tagged |
CNY 6,560.00 |
|
| RC215579L4 | Lenti ORF clone of Human G protein-coupled receptor kinase 4 (GRK4), transcript variant 3, mGFP tagged |
CNY 6,560.00 |
|
| SC300593 | GRK4 (untagged)-Human G protein-coupled receptor kinase 4 (GRK4), transcript variant 3 |
CNY 8,550.00 |
