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GRK3 (NM_005160) Human Tagged ORF Clone
CAT＃: RG215129
- TrueORF®
ADRBK2 (tGFP-tagged) - Human adrenergic, beta, receptor kinase 2 (ADRBK2)
ORF Plasmid: DDK
"NM_005160" in other vectors (8)
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CNY 9,160.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ADRBK2; BARK2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215129 representing NM_005160
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACCTGGAGGCTGTGCTGGCCGATGTCAGTTACCTGATGGCCATGGAGAAGAGCAAGGCGACCC CGGCCGCCCGCGCCAGCAAGAGGATCGTCCTGCCGGAGCCCAGTATCCGGAGTGTGATGCAGAAGTACCT TGCAGAGAGAAATGAAATAACCTTTGACAAGATTTTCAATCAGAAAATTGGTTTCTTGCTATTTAAAGAT TTTTGTTTGAATGAAATTAATGAAGCTGTACCTCAGGTGAAGTTTTATGAAGAGATAAAGGAATATGAAA AACTTGATAATGAGGAAGACCGCCTTTGCAGAAGTCGACAAATTTATGATGCCTACATCATGAAGGAACT TCTTTCCTGTTCACATCCTTTCTCAAAGCAAGCTGTAGAACACGTACAAAGTCATTTATCCAAGAAACAA GTGACATCAACTCTTTTTCAGCCATACATAGAAGAAATTTGTGAAAGCCTTCGAGGTGACATTTTTCAAA AATTTATGGAAAGTGACAAGTTCACTAGATTTTGTCAGTGGAAAAACGTTGAATTAAATATCCATTTGAC CATGAATGAGTTCAGTGTGCATAGGATTATTGGACGAGGAGGATTCGGGGAAGTTTATGGTTGCAGGAAA GCAGACACTGGAAAAATGTATGCAATGAAATGCTTAGATAAGAAGAGGATCAAAATGAAACAAGGAGAAA CATTAGCCTTAAATGAAAGAATCATGTTGTCTCTTGTCAGCACAGGAGACTGTCCTTTCATTGTATGTAT GACCTATGCCTTCCATACCCCAGATAAACTCTGCTTCATCCTGGATCTGATGAACGGGGGCGATTTGCAC TACCACCTTTCACAACACGGTGTGTTCTCTGAGAAGGAGATGCGGTTTTATGCCACTGAAATCATTCTGG GTCTGGAACACATGCACAATCGGTTTGTTGTCTACAGAGATTTGAAGCCAGCAAATATTCTCTTGGATGA ACATGGACACGCAAGAATATCAGATCTTGGTCTTGCCTGCGATTTTTCCAAAAAGAAGCCTCATGCGAGT GTTGGCACCCATGGGTACATGGCTCCCGAGGTGCTGCAGAAGGGGACGGCCTATGACAGCAGTGCCGACT GGTTCTCCCTGGGCTGCATGCTTTTCAAACTTCTGAGAGGTCACAGCCCTTTCAGACAACATAAAACCAA AGACAAGCATGAAATTGACCGAATGACACTCACCGTGAATGTGGAACTTCCAGACACCTTCTCTCCTGAA CTGAAGTCCCTTTTGGAGGGCTTGCTTCAGCGAGACGTTAGCAAGCGGCTGGGCTGTCACGGAGGCGGCT CACAGGAAGTAAAAGAGCACAGCTTTTTCAAAGGTGTTGACTGGCAGCATGTCTACTTACAAAAGTACCC ACCACCCTTGATTCCTCCCCGGGGAGAAGTCAATGCTGCTGATGCCTTTGATATTGGCTCATTTGATGAA GAGGATACCAAAGGGATTAAGCTACTTGATTGCGACCAAGAACTCTACAAGAACTTCCCTTTGGTCATCT CTGAACGCTGGCAGCAAGAAGTAACGGAAACAGTTTATGAAGCAGTAAATGCAGACACAGATAAAATCGA GGCCAGGAAGAGAGCTAAAAATAAGCAACTTGGCCACGAAGAAGATTACGCTCTGGGGAAGGACTGTATT ATGCACGGGTACATGCTGAAACTGGGAAACCCATTTCTGACTCAGTGGCAGCGTCGCTATTTTTACCTCT TTCCAAATAGACTTGAATGGAGAGGAGAGGGAGAGTCCCGGCAAAATTTACTGACAATGGAACAGATTCT CTCTGTGGAAGAAACTCAAATTAAAGACAAAAAATGCATTTTGTTCAGAATAAAAGGAGGGAAACAATTT GTCTTGCAATGTGAGAGTGATCCAGAGTTTGTGCAGTGGAAGAAAGAGTTGAACGAAACCTTCAAGGAGG CCCAGCGGCTATTGCGTCGTGCCCCGAAGTTCCTCAACAAACCTCGGTCAGGTACTGTGGAGCTCCCAAA GCCATCCCTCTGTCACAGAAACAGCAACGGCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215129 representing NM_005160
Red=Cloning site Green=Tags(s) MADLEAVLADVSYLMAMEKSKATPAARASKRIVLPEPSIRSVMQKYLAERNEITFDKIFNQKIGFLLFKD FCLNEINEAVPQVKFYEEIKEYEKLDNEEDRLCRSRQIYDAYIMKELLSCSHPFSKQAVEHVQSHLSKKQ VTSTLFQPYIEEICESLRGDIFQKFMESDKFTRFCQWKNVELNIHLTMNEFSVHRIIGRGGFGEVYGCRK ADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDLH YHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHARISDLGLACDFSKKKPHAS VGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTVNVELPDTFSPE LKSLLEGLLQRDVSKRLGCHGGGSQEVKEHSFFKGVDWQHVYLQKYPPPLIPPRGEVNAADAFDIGSFDE EDTKGIKLLDCDQELYKNFPLVISERWQQEVTETVYEAVNADTDKIEARKRAKNKQLGHEEDYALGKDCI MHGYMLKLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQNLLTMEQILSVEETQIKDKKCILFRIKGGKQF VLQCESDPEFVQWKKELNETFKEAQRLLRRAPKFLNKPRSGTVELPKPSLCHRNSNGL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005160 |
| ORF Size | 2064 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005160.4 |
| RefSeq Size | 3628 bp |
| RefSeq ORF | 2067 bp |
| Locus ID | 157 |
| UniProt ID | P35626 |
| Domains | RGS, pkinase, S_TK_X, TyrKc, PH, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Chemokine signaling pathway, Endocytosis, Olfactory transduction |
| Gene Summary | The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215129 | ADRBK2 (Myc-DDK-tagged)-Human adrenergic, beta, receptor kinase 2 (ADRBK2) |
CNY 7,560.00 |
|
| RC215129L1 | Lenti ORF clone of Human adrenergic, beta, receptor kinase 2 (ADRBK2), Myc-DDK-tagged |
CNY 9,960.00 |
|
| RC215129L2 | Lenti ORF clone of Human adrenergic, beta, receptor kinase 2 (ADRBK2), mGFP tagged |
CNY 9,120.00 |
|
| RC215129L3 | Lenti ORF clone of Human adrenergic, beta, receptor kinase 2 (ADRBK2), Myc-DDK-tagged |
CNY 9,120.00 |
|
| RC215129L4 | Lenti ORF clone of Human adrenergic, beta, receptor kinase 2 (ADRBK2), mGFP tagged |
CNY 9,960.00 |
|
| SC116916 | ADRBK2 (untagged)-Human adrenergic, beta, receptor kinase 2 (ADRBK2) |
CNY 7,568.00 |
|
| SC116917 | ADRBK2 (untagged)-ORIGENE UNIQUE VARIANT 1 of Human adrenergic, beta, receptor kinase 2 (ADRBK2) |
CNY 13,300.00 |
|
| SC323529 | ADRBK2 (untagged)-Kinase deficient mutant (K220M) of Human adrenergic, beta, receptor kinase 2 (ADRBK2) |
CNY 7,568.00 |
