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THBS3 (NM_007112) Human Tagged ORF Clone
CAT＃: RG214968
- TrueORF®
THBS3 (tGFP-tagged) - Human thrombospondin 3 (THBS3)
ORF Plasmid: DDK
"NM_007112" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | TSP3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214968 representing NM_007112
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACGCAGGAACTTCGGGGGGCCCTGGCTCTTCTCCTCCTTTGCTTTTTCACATCTGCCAGTCAGG ATCTGCAGGTAATTGACCTGCTGACTGTGGGTGAGTCTCGGCAGATGGTAGCTGTGGCAGAGAAGATCCG GACAGCCTTGCTCACTGCTGGGGACATCTACCTCTTATCCACCTTCCGCCTGCCCCCCAAGCAGGGTGGT GTCCTCTTTGGCCTCTATTCTCGCCAAGACAACACTCGATGGCTGGAGGCCTCTGTTGTAGGCAAGATCA ACAAAGTACTGGTGCGATACCAGCGGGAGGATGGCAAAGTCCACGCCGTGAACCTACAGCAAGCGGGCCT GGCTGATGGGCGCACACACACAGTTCTCCTGCGACTCCGAGGTCCCTCCAGACCCAGCCCTGCCCTACAT CTCTACGTGGACTGCAAACTGGGTGACCAACATGCAGGCCTTCCAGCACTGGCCCCCATTCCTCCAGCGG AGGTCGATGGGCTGGAGATTAGGACTGGACAGAAGGCGTATTTGAGGATGCAGGGCTTTGTGGAATCTAT GAAAATTATTCTGGGTGGGTCCATGGCCCGGGTAGGAGCCCTGAGTGAGTGTCCATTCCAAGGGGACGAG TCCATCCACAGTGCAGTGACCAATGCACTGCACTCCATTCTAGGGGAGCAGACCAAGGCGCTGGTCACCC AACTCACCCTCTTCAACCAGATCCTGGTGGAGCTGCGGGATGATATACGAGACCAGGTGAAGGAAATGTC CCTGATCCGAAACACCATTATGGAGTGTCAGGTGTGCGGCTTCCATGAGCAGCGTTCCCACTGCAGCCCC AATCCCTGCTTCCGAGGTGTGGACTGCATGGAAGTGTACGAGTACCCAGGCTACCGCTGTGGGCCCTGCC CCCCTGGCCTGCAGGGCAACGGCACCCACTGCAGTGACATCAATGAGTGTGCTCACGCTGACCCCTGTTT CCCGGGCTCCAGCTGCATCAACACCATGCCCGGCTTCCACTGTGAGGCCTGTCCTCGAGGGTACAAGGGC ACACAGGTGTCTGGTGTGGGCATTGACTATGCCCGGGCCAGCAAACAGGTCTGCAATGACATCGATGAAT GCAACGATGGCAACAATGGTGGCTGTGACCCAAACTCCATCTGCACCAACACTGTGGGCTCTTTCAAGTG TGGTCCCTGCCGCCTGGGTTTCCTGGGCAACCAGAGCCAGGGCTGCCTCCCAGCCCGGACCTGCCACAGC CCAGCCCACAGCCCCTGCCACATCCATGCTCACTGTCTCTTTGAACGCAATGGTGCAGTGTCCTGCCAGT GTAACGTGGGCTGGGCTGGGAATGGGAACGTGTGTGGGACTGACACAGACATCGATGGCTACCCAGACCA AGCACTGCCCTGCATGGACAACAACAAACACTGCAAACAGGACAACTGCCTTTTGACACCCAACTCTGGG CAGGAAGATGCTGATAATGATGGTGTGGGGGACCAGTGTGATGATGATGCTGATGGGGATGGGATCAAGA ATGTTGAGGACAACTGCCGGCTGTTCCCCAACAAAGACCAGCAGAACTCAGATACAGATTCATTTGGTGA TGCCTGTGACAATTGCCCCAACGTTCCCAACAATGACCAGAAGGACACAGATGGCAATGGGGAAGGAGAT GCCTGTGACAACGACGTGGATGGGGATGGCATCCCCAATGGATTGGACAATTGCCCTAAAGTCCCCAACC CACTACAGACAGACAGGGATGAGGACGGGGTGGGAGATGCTTGCGACAGCTGCCCTGAAATGAGCAATCC TACCCAGACAGATGCAGACAGCGACCTGGTGGGGGATGTCTGTGATACTAATGAAGACAGCGATGGGGAT GGGCATCAGGACACCAAGGACAACTGCCCACAGCTGCCAAATAGCTCCCAGCTGGACTCTGATAACGATG GACTTGGAGATGAGTGTGATGGGGATGATGACAATGATGGCATCCCAGATTATGTGCCTCCTGGTCCCGA TAACTGCCGCCTGGTACCCAATCCCAATCAGAAGGACTCAGATGGCAATGGCGTTGGTGATGTGTGTGAG GATGACTTTGACAATGATGCTGTGGTCGACCCCCTGGATGTGTGTCCTGAAAGTGCAGAGGTAACGCTTA CGGATTTTCGGGCCTATCAGACCGTCGTCCTGGATCCTGAGGGTGATGCTCAGATTGACCCAAACTGGGT TGTGCTCAACCAGGGCATGGAAATCGTTCAGACCATGAACAGTGACCCTGGCTTGGCAGTTGGATACACG GCCTTCAATGGTGTGGACTTTGAAGGCACCTTCCATGCGAACACAGTGACTGATGATGACTACGCAGGCT TTCTCTTCAGTTATCAAGACAGTGGCCGCTTCTACGTAGTCATGTGGAAGCAGACCGAGCAGACCTACTG GCAGGCTACACCCTTCCGGGCGGTTGCCCAGCCCGGGCTGCAGCTCAAGGCAGTGACATCAGTGTCTGGC CCAGGTGAGCACCTCCGAAATGCCCTGTGGCATACTGGCCACACCCCTGATCAGGTACGACTGCTGTGGA CAGACCCACGAAATGTGGGCTGGCGGGACAAGACCTCCTATCGCTGGCAGCTTCTGCACCGGCCTCAAGT TGGCTACATTCGGGTGAAGCTCTATGAGGGACCCCAGCTTGTGGCGGATTCTGGGGTGATCATTGACACA TCCATGCGAGGGGGGCGTCTTGGTGTATTCTGCTTCTCCCAAGAAAACATAATTTGGTCCAATCTCCAGT ATCGATGCAATGACACAGTGCCTGAGGACTTTGAGCCATTCCGGAGGCAGCTGCTCCAGGGAAGGGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214968 representing NM_007112
Red=Cloning site Green=Tags(s) METQELRGALALLLLCFFTSASQDLQVIDLLTVGESRQMVAVAEKIRTALLTAGDIYLLSTFRLPPKQGG VLFGLYSRQDNTRWLEASVVGKINKVLVRYQREDGKVHAVNLQQAGLADGRTHTVLLRLRGPSRPSPALH LYVDCKLGDQHAGLPALAPIPPAEVDGLEIRTGQKAYLRMQGFVESMKIILGGSMARVGALSECPFQGDE SIHSAVTNALHSILGEQTKALVTQLTLFNQILVELRDDIRDQVKEMSLIRNTIMECQVCGFHEQRSHCSP NPCFRGVDCMEVYEYPGYRCGPCPPGLQGNGTHCSDINECAHADPCFPGSSCINTMPGFHCEACPRGYKG TQVSGVGIDYARASKQVCNDIDECNDGNNGGCDPNSICTNTVGSFKCGPCRLGFLGNQSQGCLPARTCHS PAHSPCHIHAHCLFERNGAVSCQCNVGWAGNGNVCGTDTDIDGYPDQALPCMDNNKHCKQDNCLLTPNSG QEDADNDGVGDQCDDDADGDGIKNVEDNCRLFPNKDQQNSDTDSFGDACDNCPNVPNNDQKDTDGNGEGD ACDNDVDGDGIPNGLDNCPKVPNPLQTDRDEDGVGDACDSCPEMSNPTQTDADSDLVGDVCDTNEDSDGD GHQDTKDNCPQLPNSSQLDSDNDGLGDECDGDDDNDGIPDYVPPGPDNCRLVPNPNQKDSDGNGVGDVCE DDFDNDAVVDPLDVCPESAEVTLTDFRAYQTVVLDPEGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYT AFNGVDFEGTFHANTVTDDDYAGFLFSYQDSGRFYVVMWKQTEQTYWQATPFRAVAQPGLQLKAVTSVSG PGEHLRNALWHTGHTPDQVRLLWTDPRNVGWRDKTSYRWQLLHRPQVGYIRVKLYEGPQLVADSGVIIDT SMRGGRLGVFCFSQENIIWSNLQYRCNDTVPEDFEPFRRQLLQGRV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007112 |
| ORF Size | 2868 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007112.3, NP_009043.1 |
| RefSeq Size | 3176 bp |
| RefSeq ORF | 2871 bp |
| Locus ID | 7059 |
| UniProt ID | P49746 |
| Protein Families | Druggable Genome |
| Protein Pathways | ECM-receptor interaction, Focal adhesion, TGF-beta signaling pathway |
| Gene Summary | The protein encoded by this gene belongs to the thrombospondin family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentameric molecule linked by a single disulfide bond. This gene shares a common promoter with metaxin 1. Alternate splicing results in coding and non-coding transcript variants. [provided by RefSeq, Nov 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214968 | THBS3 (Myc-DDK-tagged)-Human thrombospondin 3 (THBS3) |
CNY 7,000.00 |
|
| RC214968L3 | Lenti ORF clone of Human thrombospondin 3 (THBS3), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC214968L4 | Lenti ORF clone of Human thrombospondin 3 (THBS3), mGFP tagged |
CNY 9,030.00 |
|
| SC303879 | THBS3 (untagged)-Human thrombospondin 3 (THBS3) |
CNY 7,220.00 |
