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Eph receptor B1 (EPHB1) (NM_004441) Human Tagged ORF Clone
CAT＃: RG214301
- TrueORF®
EPHB1 (tGFP-tagged) - Human EPH receptor B1 (EPHB1)
ORF Plasmid: DDK
"NM_004441" in other vectors (8)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ELK; EPHT2; Hek6; NET |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214301 representing NM_004441
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGGATTATCTACTACTGCTCCTCCTGGCATCCGCAGTGGCTGCGATGGAAGAAACGTTAATGG ACACCAGAACGGCTACTGCAGAGCTGGGCTGGACGGCCAATCCTGCGTCCGGGTGGGAAGAAGTCAGTGG CTACGATGAAAACCTGAACACCATCCGCACCTACCAGGTGTGCAATGTCTTCGAGCCCAACCAGAACAAT TGGCTGCTCACCACCTTCATCAACCGGCGGGGGGCCCATCGCATCTACACAGAGATGCGCTTCACTGTGA GAGACTGCAGCAGCCTCCCTAATGTCCCAGGATCCTGCAAGGAGACCTTCAACTTGTATTACTATGAGAC TGACTCTGTCATTGCCACCAAGAAGTCAGCCTTCTGGTCTGAGGCCCCCTACCTCAAAGTAGACACCATT GCTGCAGATGAGAGTTTCTCCCAGGTGGACTTTGGGGGAAGGCTGATGAAGGTAAACACAGAAGTCAGGA GCTTTGGGCCTCTTACTCGGAATGGTTTTTACCTCGCTTTTCAGGATTATGGAGCCTGTATGTCTCTTCT TTCTGTCCGTGTCTTCTTCAAAAAGTGTCCCAGCATTGTGCAAAATTTTGCAGTGTTTCCAGAGACTATG ACAGGGGCAGAGAGCACATCTCTGGTGATTGCTCGGGGCACATGCATCCCCAACGCAGAGGAAGTGGACG TGCCCATCAAACTCTACTGCAACGGGGATGGGGAATGGATGGTGCCTATTGGGCGATGCACCTGCAAGCC TGGCTATGAGCCTGAGAACAGCGTGGCATGCAAGGCTTGCCCTGCAGGGACATTCAAGGCCAGCCAGGAA GCTGAAGGCTGCTCCCACTGCCCCTCCAACAGCCGCTCCCCTGCAGAGGCGTCTCCCATCTGCACCTGTC GGACCGGTTATTACCGAGCGGACTTTGACCCTCCAGAAGTGGCATGCACTAGCGTCCCATCAGGTCCCCG CAATGTTATCTCCATCGTCAATGAGACGTCCATCATTCTGGAGTGGCACCCTCCAAGGGAGACAGGTGGG CGGGATGATGTGACCTACAACATCATCTGCAAAAAGTGCCGGGCAGACCGCCGGAGCTGCTCCCGCTGTG ACGACAATGTGGAGTTTGTGCCCAGGCAGCTGGGCCTGACGGAGTGCCGCGTCTCCATCAGCAGCCTGTG GGCCCACACCCCCTACACCTTTGACATCCAGGCCATCAATGGAGTCTCCAGCAAGAGTCCCTTCCCCCCA CAGCACGTCTCTGTCAACATCACCACAAACCAAGCCGCCCCCTCCACCGTTCCCATCATGCACCAAGTCA GTGCCACTATGAGGAGCATCACCTTGTCATGGCCACAGCCGGAGCAGCCCAATGGCATCATCCTGGACTA TGAGATCCGGTACTATGAGAAGGAACACAATGAGTTCAACTCCTCCATGGCCAGGAGTCAGACCAACACA GCAAGGATTGATGGGCTGCGGCCTGGCATGGTATATGTGGTACAGGTGCGTGCCCGCACTGTTGCTGGCT ACGGCAAGTTCAGTGGCAAGATGTGCTTCCAGACTCTGACTGACGATGATTACAAGTCAGAGCTGAGGGA GCAGCTGCCCCTGATTGCTGGCTCGGCAGCGGCCGGGGTCGTGTTCGTTGTGTCCTTGGTGGCCATCTCT ATCGTCTGTAGCAGGAAACGGGCTTATAGCAAAGAGGCTGTGTACAGCGATAAGCTCCAGCATTACAGCA CAGGCCGAGGCTCCCCAGGGATGAAGATCTACATTGACCCCTTCACTTACGAGGATCCCAACGAAGCTGT CCGGGAGTTTGCCAAGGAGATTGATGTATCTTTTGTGAAAATTGAAGAGGTCATCGGAGCAGGGGAGTTT GGAGAAGTGTACAAGGGGCGTTTGAAACTGCCAGGCAAGAGGGAAATCTACGTGGCCATCAAGACCCTGA AGGCAGGGTACTCGGAGAAGCAGCGTCGGGACTTTCTGAGTGAGGCGAGCATCATGGGCCAGTTCGACCA TCCTAACATCATTCGCCTGGAGGGTGTGGTCACCAAGAGTCGGCCTGTCATGATCATCACAGAGTTCATG GAGAATGGTGCATTGGATTCTTTCCTCAGGCAAAATGACGGGCAGTTCACCGTGATCCAGCTTGTGGGTA TGCTCAGGGGCATCGCTGCTGGCATGAAGTACCTGGCTGAGATGAATTATGTGCATCGGGACCTGGCTGC TAGGAACATTCTGGTCAACAGTAACCTGGTGTGCAAGGTGTCCGACTTTGGCCTCTCCCGCTACCTCCAG GATGACACCTCAGATCCCACCTACACCAGCTCCTTGGGAGGGAAGATCCCTGTGAGATGGACAGCTCCAG AGGCCATCGCCTACCGCAAGTTCACTTCAGCCAGCGACGTTTGGAGCTATGGGATCGTCATGTGGGAAGT CATGTCATTTGGAGAGAGACCCTATTGGGATATGTCCAACCAAGATGTCATCAATGCCATCGAGCAGGAC TACCGGCTGCCCCCACCCATGGACTGTCCAGCTGCTCTACACCAGCTCATGCTGGACTGTTGGCAGAAGG ACCGGAACAGCCGGCCCCGGTTTGCGGAGATTGTCAACACCCTAGATAAGATGATCCGGAACCCGGCAAG TCTCAAGACTGTGGCAACCATCACCGCCGTGCCTTCCCAGCCCCTGCTCGACCGCTCCATCCCAGACTTC ACGGCCTTTACCACCGTGGATGACTGGCTCAGCGCCATCAAAATGGTCCAGTACAGGGACAGCTTCCTCA CTGCTGGCTTCACCTCCCTCCAGCTGGTCACCCAGATGACATCAGAAGACCTCCTGAGAATAGGCATCAC CTTGGCAGGCCATCAGAAGAAGATCCTGAACAGCATTCATTCTATGAGGGTCCAGATAAGTCAGTCACCA ACGGCAATGGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214301 representing NM_004441
Red=Cloning site Green=Tags(s) MALDYLLLLLLASAVAAMEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNN WLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTI AADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETM TGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQE AEGCSHCPSNSRSPAEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGG RDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPP QHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNT ARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLPLIAGSAAAGVVFVVSLVAIS IVCSRKRAYSKEAVYSDKLQHYSTGRGSPGMKIYIDPFTYEDPNEAVREFAKEIDVSFVKIEEVIGAGEF GEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMIITEFM ENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLAEMNYVHRDLAARNILVNSNLVCKVSDFGLSRYLQ DDTSDPTYTSSLGGKIPVRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQD YRLPPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNTLDKMIRNPASLKTVATITAVPSQPLLDRSIPDF TAFTTVDDWLSAIKMVQYRDSFLTAGFTSLQLVTQMTSEDLLRIGITLAGHQKKILNSIHSMRVQISQSP TAMA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004441 |
| ORF Size | 2952 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004441.3, NP_004432.1 |
| RefSeq Size | 4536 bp |
| RefSeq ORF | 2955 bp |
| Locus ID | 2047 |
| UniProt ID | P54762 |
| Domains | pkinase, EPH_lbd, TyrKc, SAM, S_TKc, FN3 |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Axon guidance |
| Gene Summary | Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214301 | EPHB1 (Myc-DDK-tagged)-Human EPH receptor B1 (EPHB1) |
CNY 7,130.00 |
|
| RC214301L1 | Lenti ORF clone of Human EPH receptor B1 (EPHB1), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC214301L2 | Lenti ORF clone of Human EPH receptor B1 (EPHB1), mGFP tagged |
CNY 9,030.00 |
|
| RC214301L3 | Lenti ORF clone of Human EPH receptor B1 (EPHB1), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC214301L4 | Lenti ORF clone of Human EPH receptor B1 (EPHB1), mGFP tagged |
CNY 9,030.00 |
|
| RC600048 | EPHB1 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens EPH receptor B1, Signal peptide (1-17) plus EC domain (18-540) |
CNY 4,024.00 |
|
| SC117355 | EPHB1 (untagged)-Human EPH receptor B1 (EPHB1) |
CNY 7,216.00 |
|
| SC323437 | EPHB1 (untagged)-Kinase deficient mutant (K651M) of Human EPH receptor B1 (EPHB1) |
CNY 7,216.00 |
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