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HERC3 (NM_014606) Human Tagged ORF Clone
CAT＃: RG214034
- TrueORF®
HERC3 (tGFP-tagged) - Human hect domain and RLD 3 (HERC3)
ORF Plasmid: DDK
"NM_014606" in other vectors (6)
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CNY 11,500.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214034 representing NM_014606
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTATGTTGGGGATATTGGTCTCTGGGCCAACCTGGTATCAGCACCAACCTGCAGGGAATTGTGGCTG AGCCCCAGGTGTGTGGGTTCATATCTGACAGAAGTGTCAAGGAAGTGGCCTGTGGGGGAAACCACTCTGT GTTCCTGCTGGAAGATGGGGAAGTTTACACATGTGGTTTGAACACCAAGGGGCAACTGGGCCATGAGAGG GAAGGAAACAAGCCAGAACAAATTGGAGCTCTGGCAGATCAGCATATCATTCATGTGGCATGTGGCGAGT CCCACAGTCTGGCCCTCAGTGACCGAGGCCAGCTGTTTTCTTGGGGTGCAGGGAGTGATGGTCAGCTAGG ACTCATGACTACTGAGGATTCTGTGGCAGTGCCCAGGTTAATACAAAAGCTGAACCAGCAAACAATATTA CAAGTTTCCTGTGGCAACTGGCATTGCTTGGCTCTTGCGGCTGATGGCCAGTTCTTCACCTGGGGAAAGA ACAGCCATGGGCAGCTTGGCTTAGGGAAGGAGTTCCCCTCCCAAGCCAGCCCACAGAGGGTGAGGTCCCT GGAGGGGATCCCACTGGCTCAGGTGGCTGCCGGAGGGGCTCACAGCTTTGCCCTGTCTCTCTCAGGAGCT GTTTTTGGCTGGGGGATGAATAATGCCGGGCAGCTAGGGCTCAGTGATGAAAAAGATCGAGAATCTCCAT GCCATGTAAAACTCTTACGCACGCAAAAAGTTGTCTATATTAGTTGTGGAGAAGAACACACAGCAGTTCT CACAAAGAGTGGAGGTGTGTTTACCTTTGGCGCTGGTTCCTGTGGGCAACTTGGACACGACTCCATGAAT GATGAGGTTAACCCTAGAAGAGTTCTAGAGCTGATGGGTAGTGAAGTAACTCAAATTGCTTGTGGCAGAC AACATACCCTAGCCTTCGTGCCTTCTTCTGGACTCATCTATGCATTTGGTTGTGGAGCAAGAGGTCAATT AGGAACTGGGCACACTTGTAATGTTAAGTGCCCATCTCCTGTCAAGGGTTACTGGGCTGCCCACAGTGGC CAGCTTTCAGCCCGAGCTGATCGCTTTAAATATCATATCGTTAAGCAGATCTTCTCTGGAGGAGACCAGA CTTTTGTACTTTGCTCCAAATACGAGAATTATTCTCCTGCTGTTGACTTCAGGACTATGAACCAAGCACA TTATACCAGTTTAATAAATGATGAAACCATAGCAGTTTGGAGACAAAAACTCTCAGAACACAACAATGCA AATACAATCAATGGTGTTGTTCAGATATTATCTTCTGCAGCCTGTTGGAATGGAAGTTTTCTTGAAAAAA AAATTGATGAACATTTTAAAACGAGTCCCAAAATCCCTGGGATTGACCTGAACTCAACTAGGGTGTTATT TGAGAAGTTAATGAACTCTCAGCACTCCATGATTCTAGAACAGATTTTGAACAGTTTTGAAAGTTGTCTG ATTCCCCAGTTGTCAAGCTCACCACCAGATGTTGAAGCCATGAGAATCTATTTAATACTACCTGAGTTTC CCCTACTCCAGGATTCCAAGTATTATATAACATTGACTATTCCCTTGGCTATGGCCATTCTTCGGCTGGA TACAAACCCCAGCAAAGTACTAGATAACTGGTGGTCTCAGGTATGCCCGAAATATTTCATGAAGCTGGTA AACCTCTATAAAGGTGCAGTCCTTTATCTACTGAGGGGAAGAAAGACATTCTTAATTCCCGTACTGTTTA ACAATTATATCACAGCAGCTCTCAAACTCTTGGAGAAGTTATATAAGGTAAATCTTAAAGTGAAGCATGT GGAATATGATACATTTTACATTCCTGAGATTTCCAATCTCGTGGACATTCAGGAAGACTACCTCATGTGG TTCTTGCATCAAGCAGGGATGAAGGCTAGACCATCAATAATACAGGATACTGTAACACTTTGTTCCTACC CTTTCATCTTTGATGCCCAAGCCAAGACCAAAATGTTACAGACAGATGCTGAACTACAGATGCAGGTGGC AGTCAATGGAGCCAACCTGCAGAATGTCTTCATGCTTCTCACCCTGGAGCCTCTGCTGGCCAGAAGCCCC TTCCTGGTCCTTCACGTTCGCAGGAACAACCTTGTTGGAGATGCCCTAAGAGAGCTGAGCATTCATTCTG ATATTGATTTGAAAAAGCCTCTCAAAGTAATCTTTGATGGTGAAGAAGCAGTGGATGCCGGTGGTGTTAC AAAGGAATTTTTTCTTTTGCTGTTAAAAGAACTTTTGAATCCCATCTATGGAATGTTTACCTACTATCAA GATTCAAATCTCTTGTGGTTTTCAGACACGTGTTTTGTAGAGCACAACTGGTTTCACTTGATTGGTATAA CCTGTGGACTAGCTATCTACAACTCCACTGTGGTCGATCTCCACTTCCCATTGGCTCTCTACAAGAAGTT ACTCAATGTAAAGCCTGGCTTGGAAGACTTAAAGGAGTTGTCACCCACTGAAGGAAGGAGTCTCCAAGAG CTTTTAGATTACCCCGGGGAGGATGTGGAGGAGACTTTCTGCCTCAACTTCACGATCTGCCGAGAAAGCT ATGGAGTGATTGAACAGAAGAAGCTGATACCTGGGGGAGATAATGTAACTGTGTGCAAGGATAACAGGCA GGAATTTGTGGATGCTTATGTGAATTATGTCTTCCAAATCTCAGTTCATGAATGGTACACAGCCTTCTCT AGTGGCTTCCTAAAGGTGTGTGGTGGCAAAGTACTTGAGCTCTTCCAGCCTTCAGAACTGAGGGCTATGA TGGTGGGGAACAGCAACTACAACTGGGAAGAACTGGAAGAGACTGCCATCTACAAGGGAGATTACTCGGC CACACATCCCACTGTAAAACTATTTTGGGAAACATTTCATGAGTTTCCATTGGAAAAGAAGAAGAAGTTT CTCTTGTTCCTGACAGGCAGCGATCGGATTCCCATCTACGGCATGGCCAGTCTGCAGATTGTCATCCAGT CCACAGCCAGCGGGGAGGAGTACTTGCCGGTGGCCCACACTTGCTACAACCTTCTTGACCTCCCCAAGTA CAGCAGCAAAGAGATTCTGAGTGCCCGGCTGACCCAGGCCCTTGACAACTATGAAGGGTTTAGTTTGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214034 representing NM_014606
Red=Cloning site Green=Tags(s) MLCWGYWSLGQPGISTNLQGIVAEPQVCGFISDRSVKEVACGGNHSVFLLEDGEVYTCGLNTKGQLGHER EGNKPEQIGALADQHIIHVACGESHSLALSDRGQLFSWGAGSDGQLGLMTTEDSVAVPRLIQKLNQQTIL QVSCGNWHCLALAADGQFFTWGKNSHGQLGLGKEFPSQASPQRVRSLEGIPLAQVAAGGAHSFALSLSGA VFGWGMNNAGQLGLSDEKDRESPCHVKLLRTQKVVYISCGEEHTAVLTKSGGVFTFGAGSCGQLGHDSMN DEVNPRRVLELMGSEVTQIACGRQHTLAFVPSSGLIYAFGCGARGQLGTGHTCNVKCPSPVKGYWAAHSG QLSARADRFKYHIVKQIFSGGDQTFVLCSKYENYSPAVDFRTMNQAHYTSLINDETIAVWRQKLSEHNNA NTINGVVQILSSAACWNGSFLEKKIDEHFKTSPKIPGIDLNSTRVLFEKLMNSQHSMILEQILNSFESCL IPQLSSSPPDVEAMRIYLILPEFPLLQDSKYYITLTIPLAMAILRLDTNPSKVLDNWWSQVCPKYFMKLV NLYKGAVLYLLRGRKTFLIPVLFNNYITAALKLLEKLYKVNLKVKHVEYDTFYIPEISNLVDIQEDYLMW FLHQAGMKARPSIIQDTVTLCSYPFIFDAQAKTKMLQTDAELQMQVAVNGANLQNVFMLLTLEPLLARSP FLVLHVRRNNLVGDALRELSIHSDIDLKKPLKVIFDGEEAVDAGGVTKEFFLLLLKELLNPIYGMFTYYQ DSNLLWFSDTCFVEHNWFHLIGITCGLAIYNSTVVDLHFPLALYKKLLNVKPGLEDLKELSPTEGRSLQE LLDYPGEDVEETFCLNFTICRESYGVIEQKKLIPGGDNVTVCKDNRQEFVDAYVNYVFQISVHEWYTAFS SGFLKVCGGKVLELFQPSELRAMMVGNSNYNWEELEETAIYKGDYSATHPTVKLFWETFHEFPLEKKKKF LLFLTGSDRIPIYGMASLQIVIQSTASGEEYLPVAHTCYNLLDLPKYSSKEILSARLTQALDNYEGFSLA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014606 |
| ORF Size | 3150 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014606.3 |
| RefSeq Size | 4894 bp |
| RefSeq ORF | 3153 bp |
| Locus ID | 8916 |
| UniProt ID | Q15034 |
| Domains | RCC1, HECT |
| Protein Families | Druggable Genome |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214034 | HERC3 (Myc-DDK-tagged)-Human hect domain and RLD 3 (HERC3) |
CNY 7,312.00 |
|
| RC214034L1 | Lenti ORF clone of Human hect domain and RLD 3 (HERC3), Myc-DDK-tagged |
CNY 9,712.00 |
|
| RC214034L2 | Lenti ORF clone of Human hect domain and RLD 3 (HERC3), mGFP tagged |
CNY 12,350.00 |
|
| RC214034L3 | Lenti ORF clone of Human hect domain and RLD 3 (HERC3), Myc-DDK-tagged |
CNY 12,350.00 |
|
| RC214034L4 | Lenti ORF clone of Human hect domain and RLD 3 (HERC3), mGFP tagged |
CNY 12,350.00 |
|
| SC114951 | HERC3 (untagged)-Human hect domain and RLD 3 (HERC3) |
CNY 7,320.00 |
