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TRPM1 (NM_002420) Human Tagged ORF Clone
CAT＃: RG213443
- TrueORF®
TRPM1 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily M, member 1 (TRPM1)
ORF Plasmid: DDK
"NM_002420" in other vectors (6)
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CNY 18,048.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CSNB1C; LTRPC1; MLSN1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213443 representing NM_002420
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGACTCTAACAGGTGTTGCTGTGGCCAGTTCACCAACCAGCATATCCCCCCTCTGCCAAGTGCAA CACCCAGCAAAAATGAAGAGGAAAGCAAACAGGTGGAGACTCAGCCTGAGAAATGGTCTGTTGCCAAGCA CACCCAGAGCTACCCAACAGATTCCTATGGAGTTCTTGAATTCCAGGGTGGCGGATATTCCAATAAAGCC ATGTATATCCGTGTATCCTATGACACCAAGCCAGACTCACTGCTCCATCTCATGGTGAAAGATTGGCAGC TGGAACTCCCCAAGCTCTTAATATCTGTGCATGGAGGCCTCCAGAACTTTGAGATGCAGCCCAAGCTGAA ACAAGTCTTTGGGAAAGGCCTGATCAAGGCTGCTATGACCACCGGGGCCTGGATCTTCACCGGGGGTGTC AGCACAGGTGTTATCAGCCACGTAGGGGATGCCTTGAAAGACCACTCCTCCAAGTCCAGAGGCCGGGTTT GTGCTATAGGAATTGCTCCATGGGGCATCGTGGAGAATAAGGAAGACCTGGTTGGAAAGGATGTAACAAG AGTGTACCAGACCATGTCCAACCCTCTAAGTAAGCTCTCTGTGCTCAACAACTCCCACACCCACTTCATC CTGGCTGACAATGGCACCCTGGGCAAGTATGGCGCCGAGGTGAAGCTGCGAAGGCTGCTGGAAAAGCACA TCTCCCTGCAGAAGATCAACACAAGACTGGGGCAGGGCGTGCCCCTCGTGGGTCTCGTGGTGGAGGGGGG CCCTAACGTGGTGTCCATCGTCTTGGAATACCTGCAAGAAGAGCCTCCCATCCCTGTGGTGATTTGTGAT GGCAGCGGACGTGCCTCGGACATCCTGTCCTTTGCGCACAAGTACTGTGAAGAAGGCGGAATAATAAATG AGTCCCTCAGGGAGCAGCTTCTAGTTACCATTCAGAAAACATTTAATTATAATAAGGCACAATCACATCA GCTGTTTGCAATTATAATGGAGTGCATGAAGAAGAAAGAACTCGTCACTGTGTTCAGAATGGGTTCTGAG GGCCAGCAGGACATCGAGATGGCAATTTTAACTGCCCTGCTGAAAGGAACAAACGTATCTGCTCCAGATC AGCTGAGCTTGGCACTGGCTTGGAACCGCGTGGACATAGCACGAAGCCAGATCTTTGTCTTTGGGCCCCA CTGGCCGCCCCTGGGAAGCCTGGCACCCCCGACGGACAGCAAAGCCACGGAGAAGGAGAAGAAGCCACCC ATGGCCACCACCAAGGGAGGAAGAGGAAAAGGGAAAGGCAAGAAGAAAGGGAAAGTGAAAGAGGAAGTGG AGGAAGAAACTGACCCCCGGAAGATAGAGCTGCTGAACTGGGTGAATGCTTTGGAGCAAGCGATGCTAGA TGCTTTAGTCTTAGATCGTGTCGACTTTGTGAAGCTCCTGATTGAAAACGGAGTGAACATGCAACACTTT CTGACCATTCCGAGGCTGGAGGAGCTTTATAACACAAGACTGGGTCCACCAAACACACTTCATCTGCTGG TGAGGGATGTGAAAAAGAGCAACCTTCCGCCTGATTACCACATCAGCCTCATAGACATCGGGCTCGTGCT GGAGTACCTCATGGGAGGAGCCTACCGCTGCAACTACACTCGGAAAAACTTTCGGACCCTTTACAACAAC TTGTTTGGACCAAAGAGGCCTAAAGCTCTTAAACTTCTGGGAATGGAAGATGATGAGCCTCCAGCTAAAG GGAAGAAAAAGAAAAAGAAGAAAAAGGAGGAAGAGATCGACATTGATGTGGACGACCCTGCCGTGAGTCG GTTCCAGTATCCCTTCCACGAGCTGATGGTGTGGGCAGTGCTGATGAAACGCCAGAAAATGGCAGTGTTC CTCTGGCAGCGAGGGGAAGAGAGCATGGCCAAGGCCCTGGTGGCCTGCAAGCTCTACAAGGCCATGGCCC ACGAGTCCTCCGAGAGTGATCTGGTGGATGACATCTCCCAGGACTTGGATAACAATTCCAAAGACTTCGG CCAGCTTGCTTTGGAGTTATTAGACCAGTCCTATAAGCATGACGAGCAGATCGCTATGAAACTCCTGACC TACGAGCTGAAAAACTGGAGCAACTCGACCTGCCTCAAACTGGCCGTGGCAGCCAAACACCGGGACTTCA TTGCTCACACCTGCAGCCAGATGCTGCTGACCGATATGTGGATGGGAAGACTGCGGATGCGGAAGAACCC CGGCCTGAAGGTTATCATGGGGATTCTTCTACCCCCCACCATCTTGTTTTTGGAATTTCGCACATATGAT GATTTCTCGTATCAAACATCCAAGGAAAATGAGGATGGCAAAGAAAAAGAAGAGGAAAATACGGATGCAA ATGCAGATGCTGGCTCAAGAAAGGGGGATGAGGAGAACGAGCACAAAAAACAGAGAAGTATTCCCATCGG AACAAAGATCTGTGAATTCTATAACGCGCCCATTGTCAAGTTCTGGTTTTACACAATATCATACTTGGGC TACCTGCTGCTGTTTAACTACGTCATCCTGGTGCGGATGGATGGCTGGCCGTCCCTCCAGGAGTGGATCG TCATCTCCTACATCGTGAGCCTGGCGTTAGAGAAGATACGAGAGATCCTCATGTCAGAACCAGGCAAACT CAGCCAGAAAATCAAAGTTTGGCTTCAGGAGTACTGGAACATCACAGATCTCGTGGCCATTTCCACATTC ATGATTGGAGCAATTCTTCGCCTACAGAACCAGCCCTACATGGGCTATGGCCGGGTGATCTACTGTGTGG ATATCATCTTCTGGTACATCCGTGTCCTGGACATCTTTGGTGTCAACAAGTATCTGGGGCCATACGTGAT GATGATTGGAAAGATGATGATCGACATGCTGTACTTTGTGGTCATCATGCTGGTCGTGCTCATGAGTTTC GGAGTAGCCCGTCAAGCCATTCTGCATCCAGAGGAGAAGCCCTCTTGGAAACTGGCCCGAAACATCTTCT ACATGCCCTACTGGATGATCTATGGAGAGGTGTTTGCAGACCAGATAGACCTCTACGCCATGGAAATTAA TCCTCCTTGTGGTGAGAACCTATATGATGAGGAGGGCAAGCGGCTTCCTCCCTGTATCCCCGGCGCCTGG CTCACTCCAGCACTCATGGCGTGCTATCTACTGGTCGCCAACATCCTGCTGGTGAACCTGCTGATTGCTG TGTTCAACAATACCTTCTTTGAAGTAAAATCAATATCCAACCAGGTGTGGAAGTTCCAGCGATATCAGCT GATTATGACATTTCATGACAGGCCAGTCCTGCCCCCACCGATGATCATTTTAAGCCACATCTACATCATC ATTATGCGTCTCAGCGGCCGCTGCAGGAAAAAGAGAGAAGGGGACCAAGAGGAACGGGATCGTGGATTGA AGCTCTTCCTTAGCGACGAGGAGCTAAAGAGGCTGCATGAGTTCGAGGAGCAGTGCGTGCAGGAGCACTT CCGGGAGAAGGAGGATGAGCAGCAGTCGTCCAGCGACGAGCGCATCCGGGTCACTTCTGAAAGAGTTGAA AATATGTCAATGAGGTTGGAAGAAATCAATGAAAGAGAAACTTTTATGAAAACTTCCCTGCAGACTGTTG ACCTTCGACTTGCTCAGCTAGAAGAATTATCTAACAGAATGGTGAATGCTCTTGAAAATCTTGCGGGAAT CGACAGGTCTGACCTGATCCAGGCACGGTCCCGGGCTTCTTCTGAATGTGAGGCAACGTATCTTCTCCGG CAAAGCAGCATCAATAGCGCTGATGGCTACAGCTTGTATCGATATCATTTTAACGGAGAAGAGTTATTAT TTGAGGATACATCTCTCTCCACGTCACCAGGGACAGGAGTCAGGAAAAAAACCTGTTCCTTCCGTATAAA GGAAGAGAAGGACGTGAAAACGCACCTAGTCCCAGAATGTCAGAACAGTCTTCACCTTTCACTGGGCACA AGCACATCAGCAACCCCAGATGGCAGTCACCTTGCAGTAGATGACTTAAAGAACGCTGAAGAGTCAAAAT TAGGTCCAGATATTGGGATTTCAAAGGAAGATGATGAAAGACAGACAGACTCTAAAAAAGAAGAAACTAT TTCCCCAAGTTTAAATAAAACAGATGTGATACATGGACAGGACAAATCAGATGTTCAAAACACTCAGCTA ACAGTGGAAACGACAAATATAGAAGGCACTATTTCCTATCCCCTGGAAGAAACCAAAATTACACGCTATT TCCCCGATGAAACGATCAATGCTTGTAAAACAATGAAGTCCAGAAGCTTCGTCTATTCCCGGGGAAGAAA GCTGGTCGGTGGGGTTAACCAGGATGTAGAGTACAGTTCAATCACGGACCAGCAATTGACGACGGAATGG CAATGCCAAGTTCAAAAGATCACGCGCTCTCATAGCACAGATATTCCTTACATTGTGTCGGAAGCTGCAG TGCAAGCTGAGCATAAAGAGCAGTTTGCAGATATGCAAGATGAACACCATGTCGCTGAAGCAATTCCTCG AATCCCTCGCTTGTCCCTAACCATTACTGACAGAAATGGGATGGAAAACTTACTGTCTGTGAAGCCAGAT CAAACTTTGGGATTCCCATCTCTCAGGTCAAAAAGTTTACATGGACATCCTAGGAATGTGAAATCCATTC AGGGAAAGTTAGACAGATCTGGACATGCCAGTAGTGTAAGCAGCTTAGTAATTGTGTCTGGAATGACAGC AGAAGAAAAAAAGGTTAAGAAAGAGAAAGCTTCCACAGAAACTGAATGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213443 representing NM_002420
Red=Cloning site Green=Tags(s) MKDSNRCCCGQFTNQHIPPLPSATPSKNEEESKQVETQPEKWSVAKHTQSYPTDSYGVLEFQGGGYSNKA MYIRVSYDTKPDSLLHLMVKDWQLELPKLLISVHGGLQNFEMQPKLKQVFGKGLIKAAMTTGAWIFTGGV STGVISHVGDALKDHSSKSRGRVCAIGIAPWGIVENKEDLVGKDVTRVYQTMSNPLSKLSVLNNSHTHFI LADNGTLGKYGAEVKLRRLLEKHISLQKINTRLGQGVPLVGLVVEGGPNVVSIVLEYLQEEPPIPVVICD GSGRASDILSFAHKYCEEGGIINESLREQLLVTIQKTFNYNKAQSHQLFAIIMECMKKKELVTVFRMGSE GQQDIEMAILTALLKGTNVSAPDQLSLALAWNRVDIARSQIFVFGPHWPPLGSLAPPTDSKATEKEKKPP MATTKGGRGKGKGKKKGKVKEEVEEETDPRKIELLNWVNALEQAMLDALVLDRVDFVKLLIENGVNMQHF LTIPRLEELYNTRLGPPNTLHLLVRDVKKSNLPPDYHISLIDIGLVLEYLMGGAYRCNYTRKNFRTLYNN LFGPKRPKALKLLGMEDDEPPAKGKKKKKKKKEEEIDIDVDDPAVSRFQYPFHELMVWAVLMKRQKMAVF LWQRGEESMAKALVACKLYKAMAHESSESDLVDDISQDLDNNSKDFGQLALELLDQSYKHDEQIAMKLLT YELKNWSNSTCLKLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNPGLKVIMGILLPPTILFLEFRTYD DFSYQTSKENEDGKEKEEENTDANADAGSRKGDEENEHKKQRSIPIGTKICEFYNAPIVKFWFYTISYLG YLLLFNYVILVRMDGWPSLQEWIVISYIVSLALEKIREILMSEPGKLSQKIKVWLQEYWNITDLVAISTF MIGAILRLQNQPYMGYGRVIYCVDIIFWYIRVLDIFGVNKYLGPYVMMIGKMMIDMLYFVVIMLVVLMSF GVARQAILHPEEKPSWKLARNIFYMPYWMIYGEVFADQIDLYAMEINPPCGENLYDEEGKRLPPCIPGAW LTPALMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHDRPVLPPPMIILSHIYII IMRLSGRCRKKREGDQEERDRGLKLFLSDEELKRLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVE NMSMRLEEINERETFMKTSLQTVDLRLAQLEELSNRMVNALENLAGIDRSDLIQARSRASSECEATYLLR QSSINSADGYSLYRYHFNGEELLFEDTSLSTSPGTGVRKKTCSFRIKEEKDVKTHLVPECQNSLHLSLGT STSATPDGSHLAVDDLKNAEESKLGPDIGISKEDDERQTDSKKEETISPSLNKTDVIHGQDKSDVQNTQL TVETTNIEGTISYPLEETKITRYFPDETINACKTMKSRSFVYSRGRKLVGGVNQDVEYSSITDQQLTTEW QCQVQKITRSHSTDIPYIVSEAAVQAEHKEQFADMQDEHHVAEAIPRIPRLSLTITDRNGMENLLSVKPD QTLGFPSLRSKSLHGHPRNVKSIQGKLDRSGHASSVSSLVIVSGMTAEEKKVKKEKASTETEC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002420 |
| ORF Size | 4809 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002420.6 |
| RefSeq Size | 5428 bp |
| RefSeq ORF | 4812 bp |
| Locus ID | 4308 |
| UniProt ID | Q7Z4N2 |
| Domains | ion_trans |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| Gene Summary | This gene encodes a member of the transient receptor potential melastatin subfamily of transient receptor potential ion channels. The encoded protein is a calcium permeable cation channel that is expressed in melanocytes and may play a role in melanin synthesis. Specific mutations in this gene are the cause autosomal recessive complete congenital stationary night blindness-1C. The expression of this protein is inversely correlated with melanoma aggressiveness and as such it is used as a prognostic marker for melanoma metastasis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213443 | TRPM1 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily M, member 1 (TRPM1) |
CNY 16,448.00 |
|
| RC213443L1 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 1 (TRPM1), Myc-DDK-tagged |
CNY 18,848.00 |
|
| RC213443L2 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 1 (TRPM1), mGFP tagged |
CNY 17,860.00 |
|
| RC213443L3 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 1 (TRPM1), Myc-DDK-tagged |
CNY 17,860.00 |
|
| RC213443L4 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 1 (TRPM1), mGFP tagged |
CNY 17,860.00 |
|
| SC318643 | TRPM1 (untagged)-Human transient receptor potential cation channel, subfamily M, member 1 (TRPM1) |
CNY 30,880.00 |
