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TPCN1 (NM_017901) Human Tagged ORF Clone
CAT＃: RG213190
- TrueORF®
TPCN1 (tGFP-tagged) - Human two pore segment channel 1 (TPCN1), transcript variant 2
ORF Plasmid: DDK
"NM_017901" in other vectors (4)
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CNY 10,560.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | TPC1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213190 representing NM_017901
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGAGTTTGGATGACGACGTGCCGCTCATCCTGACCTTGGATGAGGGTGGCAGTGCCCCACTGG CTCCCTCCAACGGCCTGGGCCAAGAAGAGCTACCTAGCAAAAATGGCGGCAGCTATGCCATCCACGACTC CCAGGCCCCCAGTCTCAGCTCTGGGGGTGAGAGTTCCCCCTCCAGCCCCGCACACAACTGGGAGATGAAT TACCAAGAGGCAGCAATCTACCTCCAGGAAGGCGAGAACAACGACAAGTTCTTCACCCACCCCAAGGATG CCAAGGCGCTGGCGGCCTACCTCTTTGCACACAATCACCTCTTCTACCTGATGGAGCTGGCCACGGCCCT GCTGCTGCTGCTGCTCTCCCTGTGCGAGGCCCCCGCCGTCCCCGCACTCCGGCTTGGCATCTATGTCCAC GCCACCCTGGAGCTGTTTGCCCTGATGGTGGTAGTGTTTGAACTCTGCATGAAGTTACGCTGGCTGGGCC TCCACACCTTCATCCGGCACAAGCGGACCATGGTCAAGACCTCGGTGCTGGTGGTGCAGTTTGTCGAGGC CATCGTGGTGTTGGTACGGCAGATGTCCCATGTGCGGGTGACCCGAGCACTGCGCTGCATTTTCCTGGTG GACTGTCGGTATTGCGGTGGCGTCCGGCGCAACCTGCGGCAGATCTTCCAGTCCCTGCCGCCCTTCATGG ACATCCTCCTGCTGCTGCTGTTCTTCATGATCATCTTTGCCATCCTCGGTTTCTACTTGTTCTCCCCTAA CCCTTCAGACCCCTACTTCAGCACCCTGGAGAACAGCATCGTCAGTCTGTTTGTCCTTCTGACCACAGCC AATTTCCCAGATGTGATGATGCCCTCCTACTCCCGGAACCCCTGGTCCTGCGTCTTCTTCATCGTGTACC TCTCCATCGAGCTGTATTTCATCATGAACCTGCTTCTGGCTGTGGTGTTCGACACCTTCAATGACATTGA GAAACGCAAGTTCAAGTCTTTGCTACTGCACAAGCGAACCGCTATCCAGCATGCCTACCGCCTGCTCATC AGCCAGAGGAGGCCTGCCGGCATCTCCTACAGGCAGTTTGAAGGCCTCATGCGCTTCTACAAGCCCCGGA TGAGTGCCAGGGAGCGCTATCTTACCTTCAAGGCCCTGAATCAGAACAACACACCCCTGCTCAGCCTAAA GGACTTTTACGATATCTACGAAGTTGCTGCTTTGAAGTGGAAGGCCAAGAAAAACAGAGAGCACTGGTTT GATGAGCTTCCCAGGACGGCGCTCCTCATCTTCAAAGGTATTAATATCCTTGTGAAGTCCAAGGCCTTCC AGTATTTCATGTACTTGGTGGTGGCAGTCAACGGGGTCTGGATCCTCGTGGAGACATTTATGCTGAAAGG TGGGAACTTCTTCTCCAAGCACGTGCCCTGGAGTTACCTCGTCTTTCTAACTATCTATGGGGTGGAGCTG TTCCTGAAGGTTGCCGGCCTGGGCCCTGTGGAGTACTTGTCTTCCGGATGGAACTTGTTTGACTTCTCCG TGACAGTGTTCGCCTTCCTGGGACTGCTGGCGCTGGCCCTCAACATGGAGCCCTTCTATTTCATCGTGGT CCTGCGCCCCCTCCAGCTGCTGAGGTTGTTTAAGTTGAAGGAGCGCTACCGCAACGTGCTGGACACCATG TTCGAGCTGCTGCCCCGGATGGCCAGCCTGGGCCTCACCCTGCTCATCTTTTACTACTCCTTCGCCATCG TGGGCATGGAGTTCTTCTGCGGGATCGTCTTCCCCAACTGCTGCAACACGAGTACAGTGGCAGATGCCTA CCGCTGGCGCAACCACACCGTGGGCAACAGGACCGTGGTGGAGGAAGGCTACTATTATCTCAATAATTTT GACAACATCCTCAACAGCTTTGTGACCCTGTTTGAGCTCACAGTTGTCAACAACTGGTACATCATCATGG AAGGCGTCACCTCTCAGACCTCCCACTGGAGCCGCCTCTACTTCATGACCTTTTACATTGTGACCATGGT GGTGATGACGATCATTGTCGCCTTTATCCTCGAGGCCTTCGTCTTCCGAATGAACTACAGCCGCAAGAAC CAGGACTCGGAAGTTGATGGTGGCATCACCCTTGAGAAGGAAATCTCCAAAGAAGAGCTGGTTGCCGTCC TGGAGCTCTACCGGGAGGCACGGGGGGCCTCCTCGGATGTCACCAGGCTGCTGGAGACCCTCTCCCAGAT GGAGAGATACCAGCAACATTCCATGGTGTTTCTGGGACGGCGATCAAGGACCAAGAGCGACCTGAGCCTG AAGATGTACCAGGAGGAGATCCAGGAGTGGTATGAGGAGCATGCCAGGGAGCAAGAGCAGCAGCGACAAC TCAGCAGCAGTGCAGCCCCCGCCGCCCAGCAGCCCCCAGGCAGCCGCCAGCGCTCCCAGACCGTTACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213190 representing NM_017901
Red=Cloning site Green=Tags(s) MAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSPSSPAHNWEMN YQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVH ATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLV DCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTA NFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLI SQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWF DELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVEL FLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTM FELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNF DNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKN QDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSL KMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017901 |
| ORF Size | 2448 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017901.6 |
| RefSeq Size | 5379 bp |
| RefSeq ORF | 2451 bp |
| Locus ID | 53373 |
| UniProt ID | Q9ULQ1 |
| Protein Families | Druggable Genome, Ion Channels: Other, Transmembrane |
| Gene Summary | Voltage-gated Ca(2+) and Na+ channels have 4 homologous domains, each containing 6 transmembrane segments, S1 to S6. TPCN1 is similar to these channels, but it has only 2 domains containing S1 to S6 (Ishibashi et al., 2000 [PubMed 10753632]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213190 | TPCN1 (Myc-DDK-tagged)-Human two pore segment channel 1 (TPCN1), transcript variant 2 |
CNY 8,960.00 |
|
| RC213190L3 | Lenti-ORF clone of TPCN1 (Myc-DDK-tagged)-Human two pore segment channel 1 (TPCN1), transcript variant 2 |
CNY 5,890.00 |
|
| RC213190L4 | Lenti-ORF clone of TPCN1 (mGFP-tagged)-Human two pore segment channel 1 (TPCN1), transcript variant 2 |
CNY 5,890.00 |
|
| SC314764 | TPCN1 (untagged)-Human two pore segment channel 1 (TPCN1), transcript variant 2 |
CNY 7,220.00 |
