ST8SIA5 (NM_013305) Human Tagged ORF Clone

CAT#: RG213009

  • TrueORF®

ST8SIA5 (tGFP-tagged) - Human ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 (ST8SIA5)

ORF Plasmid: DDK tGFP



  "NM_013305" in other vectors (6)

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CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms SIAT8-E; SIAT8E; ST8SiaV
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG213009 representing NM_013305
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCTACGCGGACCCCTCGGCCAACCGGGATTTGTTGGGGAGCCGAACTTTGCTCTTCATCTTCATCT
GCGCCTTTGCCTTGGTGACCTTGCTGCAACAGATCCTGTATGGCAGGAACTACATTAAGAGGTACTTTGA
ATTTTATGAGGGGCCTTTTGAATATAACTCCACAAGATGCCTGGAGCTGAGGCACGAAATATTGGAAGTG
AAGGTGCTGTCCATGGTGAAGCAGTCAGAGCTGTTCGACAGGTGGAAGAGCCTCCAGATGTGCAAATGGG
CGATGAACATCTCTGAGGCCAACCAGTTCAAGTCTACTCTGTCCAGGTGCTGCAACGCCCCTGCCTTTCT
CTTCACCACCCAGAAGAACACTCCCCTGGGGACAAAGCTCAAGTATGAGGTGGACACCAGTGGCATCTAC
CACATCAACCAGGAGATCTTCCGCATGTTTCCCAAGGACATGCCCTACTACCGGTCCCAGTTTAAGAAGT
GTGCTGTAGTGGGCAACGGAGGCATCTTGAAGAACAGCCGCTGCGGGAGGGAGATCAACAGCGCCGACTT
CGTCTTCCGGTGCAACCTGCCCCCCATCTCAGAGAAGTACACCATGGATGTGGGGGTGAAGACGGATGTG
GTCACTGTGAACCCCAGCATCATCACAGAGAGGTTCCACAAGCTGGAGAAGTGGCGGCGGCCGTTCTATC
GCGTGCTGCAGGTGTACGAGAACGCGTCGGTGCTGCTGCCTGCCTTCTACAACACGCGCAACACCGACGT
GTCCATCCGCGTCAAGTACGTGCTGGACGACTTCGAATCGCCGCAAGCTGTCTACTACTTCCATCCGCAG
TACCTGGTCAACGTGTCGCGCTACTGGCTCAGCCTGGGGGTGCGCGCCAAGCGCATCAGCACCGGCCTCA
TTCTGGTCACTGCGGCGCTGGAGCTCTGTGAGGAGGTGCACCTCTTTGGCTTCTGGGCCTTCCCCATGAA
CCCCTCGGGCCTCTACATCACTCACCACTACTATGACAACGTCAAGCCGCGTCCCGGCTTCCACGCCATG
CCCTCTGAGATCTTCAACTTCCTGCACTTGCACAGCCGAGGCATCCTCCGCGTGCACACGGGCACCTGCA
GCTGCTGC


AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG213009 representing NM_013305
Red=Cloning site Green=Tags(s)

MRYADPSANRDLLGSRTLLFIFICAFALVTLLQQILYGRNYIKRYFEFYEGPFEYNSTRCLELRHEILEV
KVLSMVKQSELFDRWKSLQMCKWAMNISEANQFKSTLSRCCNAPAFLFTTQKNTPLGTKLKYEVDTSGIY
HINQEIFRMFPKDMPYYRSQFKKCAVVGNGGILKNSRCGREINSADFVFRCNLPPISEKYTMDVGVKTDV
VTVNPSIITERFHKLEKWRRPFYRVLQVYENASVLLPAFYNTRNTDVSIRVKYVLDDFESPQAVYYFHPQ
YLVNVSRYWLSLGVRAKRISTGLILVTAALELCEEVHLFGFWAFPMNPSGLYITHHYYDNVKPRPGFHAM
PSEIFNFLHLHSRGILRVHTGTCSCC

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_013305
ORF Size 1128 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_013305.6
RefSeq Size 2627 bp
RefSeq ORF 1131 bp
Locus ID 29906
UniProt ID O15466
Protein Families Transmembrane
Protein Pathways Glycosphingolipid biosynthesis - ganglio series, Metabolic pathways
Gene Summary The protein encoded by this gene is a type II membrane protein that may be present in the Golgi apparatus. The encoded protein, which is a member of glycosyltransferase family 29, may be involved in the synthesis of gangliosides GD1c, GT1a, GQ1b, and GT3 from GD1a, GT1b, GM1b, and GD3, respectively. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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