MPG (NM_001015052) Human Tagged ORF Clone

CAT#: RG212812

  • TrueORF®

MPG (tGFP-tagged) - Human N-methylpurine-DNA glycosylase (MPG), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_001015052" in other vectors (4)

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CNY 4,370.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-MPG Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms AAG; ADPG; anpg; APNG; CRA36.1; MDG; Mid1; PIG11; PIG16
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG212812 representing NM_001015052
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCGCGCGCAGCGGGGCCCAGTTTTGCCGACGGATGGGGCAAAAGAAGCAGCGACCAGCTAGAGCAG
GGCAGCCACACAGCTCGTCCGACGCAGCCCAGGCACCTGCAGAGCAGCCACACAGCTCGTCCGATGCAGC
CCAGGCACCTTGCCCCAGGGAGCGCTGCTTGGGACCGCCCACCACTCCGGGCCCATACCGCAGCATCTAT
TTCTCAAGCCCAAAGGGCCACCTTACCCGACTGGGGTTGGAGTTCTTCGACCAGCCGGCAGTCCCCCTGG
CCCGGGCATTTCTGGGACAGGTCCTAGTCCGGCGACTTCCTAATGGCACAGAACTCCGAGGCCGCATCGT
GGAGACCGAGGCATACCTGGGGCCAGAGGATGAAGCCGCCCACTCAAGGGGTGGCCGGCAGACCCCCCGC
AACCGAGGCATGTTCATGAAGCCGGGGACCCTGTACGTGTACATCATTTACGGCATGTACTTCTGCATGA
ACATCTCCAGCCAGGGGGACGGGGCTTGCGTCTTGCTGCGAGCACTGGAGCCCCTGGAAGGTCTGGAGAC
CATGCGTCAGCTTCGCAGCACCCTCCGGAAAGGCACCGCCAGCCGTGTCCTCAAGGACCGCGAGCTCTGC
AGTGGCCCCTCCAAGCTGTGCCAGGCCCTGGCCATCAACAAGAGCTTTGACCAGAGGGACCTGGCACAGG
ATGAAGCTGTATGGCTGGAGCGTGGTCCCCTGGAGCCCAGTGAGCCGGCTGTAGTGGCAGCAGCCCGGGT
GGGCGTCGGCCATGCAGGGGAGTGGGCCCGGAAACCCCTCCGCTTCTATGTCCGGGGCAGCCCCTGGGTC
AGTGTGGTCGACAGAGTGGCTGAGCAGGACACACAGGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG212812 representing NM_001015052
Red=Cloning site Green=Tags(s)

MPARSGAQFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSSDAAQAPCPRERCLGPPTTPGPYRSIY
FSSPKGHLTRLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPR
NRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELC
SGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRGSPWV
SVVDRVAEQDTQA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001015052
ORF Size 879 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001015052.3
RefSeq Size 1096 bp
RefSeq ORF 882 bp
Locus ID 4350
UniProt ID P29372
Protein Families Druggable Genome, Transmembrane
Protein Pathways Base excision repair
Gene Summary Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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