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NSD3 (WHSC1L1) (NM_017778) Human Tagged ORF Clone
CAT＃: RG212790
- TrueORF®
WHSC1L1 (tGFP-tagged) - Human Wolf-Hirschhorn syndrome candidate 1-like 1 (WHSC1L1), transcript variant short
ORF Plasmid: DDK
"NM_017778" in other vectors (4)
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CNY 5,040.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212790 representing NM_017778
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTCTCTTTCTCTTTCATGCAAGGGATCATGGGAAACACAATTCAGCAACCACCTCAACTCATTG ACTCCGCCAACATCCGTCAGGAGGATGCCTTTGATAACAACAGTGACATTGCTGAAGATGGTGGCCAGAC ACCATATGAAGCTACTTTGCAGCAAGGCTTTCAGTACCCAGCTACAACAGAAGATCTTCCTCCACTCACA AATGGGTATCCATCATCAATCAGTGTGTATGAAACTCAAACCAAATACCAGTCATATAATCAGTATCCTA ATGGGTCAGCCAATGGCTTTGGTGCAGTTAGAAACTTTAGCCCCACTGACTATTATCATTCAGAAATTCC AAACACAAGACCACATGAAATTCTGGAAAAACCTTCCCCTCCACAGCCACCACCTCCTCCTTCGGTACCA CAAACTGTGATTCCAAAGAAGACTGGCTCACCTGAAATTAAACTAAAAATAACCAAAACTATCCAGAATG GCAGGGAATTGTTTGAGTCTTCCCTTTGTGGAGACCTTTTAAATGAAGTACAGGCAAGTGAGCACACGAA ATCAAAGCATGAAAGCAGAAAAGAAAAGAGGAAAAAAAGCAACAAGCATGACTCATCAAGATCTGAAGAG CGCAAGTCACACAAAATCCCCAAATTAGAACCAGAGGAACAAAATAGACCAAATGAGAGGGTTGACACTG TATCAGAAAAACCAAGGGAAGAACCAGTACTAAAAGAGGAAGCCCCAGTTCAGCCAATACTATCTTCTGT TCCAACAACGGAAGTGTCCACTGGTGTTAAGTTTCAGGTTGGCGATCTTGTGTGGTCCAAGGTGGGAACC TATCCTTGGTGGCCTTGTATGGTTTCAAGTGATCCCCAGCTTGAGGTTCATACTAAAATTAACACAAGAG GTGCCCGAGAATATCATGTCCAGTTTTTTAGCAACCAGCCAGAGAGGGCGTGGGTTCATGAAAAACGGGT ACGAGAGTATAAAGGTCATAAACAGTATGAAGAATTACTGGCTGAGGCAACCAAACAAGCCAGCAATCAC TCTGAGAAACAAAAGATTCGGAAACCCCGACCTCAGAGAGAACGTGCTCAGTGGGATATTGGCATTGCCC ATGCAGAGAAAGCATTGAAAATGACTCGAGAAGAAAGAATAGAACAGTATACTTTTATTTACATTGATAA ACAGCCTGAAGAGGCTTTATCCCAAGCAAAAAAGAGTGTTGCCTCCAAAACCGAAGTTAAAAAAACCCGA CGACCAAGATCTGTGCTGAATACTCAGCCAGAACAGACCAATGCAGGGGAGGTGGCCTCCTCACTCTCAA GTACTGAAATTCGGAGACATAGCCAGAGGCGGCACACAAGTGCGGAAGAGGAAGAGCCACCGCCTGTTAA AATAGCCTGGAAAACTGCGGCAGCAAGGAAATCCTTACCAGCTTCCATTACGATGCACAAAGGGAGCCTG GATTTGCAGAAGTGTAACATGTCTCCAGTTGTGAAAATTGAACAAGTGTTTGCTCTTCAGAATGCTACAG GGGATGGGAAATTTATCGATCAATTTGTTTATTCAACAAAGGGAATTGGTAACAAAACAGAAATAAGTGT CAGGGGGCAAGACAGGCTTATAATTTCTACACCAAACCAGAGAAATGAAAAGCCAACGCAGAGTGTATCA TCTCCTGAAGCAACATCTGGTTCTACAGGCTCAGTAGAAAAGAAGCAACAGAGAAGATCAATTAGAACTC GTTCTGAATCAGAGAAATCCACTGAGGTTGTGCCAAAGAAGAAGATCAAAAAGGAGCAGGTTGAAACAGT TCCTCAGGCTACAGTGAAGACTGGATTACAGAAAGGGTCGGCGGACCGGGGAGTGCAGGGCTCTGTCAGA TTCAGTGACAGCTCCGTCTCCGCAGCGATTGAGGAAACTGTGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212790 representing NM_017778
Red=Cloning site Green=Tags(s) MDFSFSFMQGIMGNTIQQPPQLIDSANIRQEDAFDNNSDIAEDGGQTPYEATLQQGFQYPATTEDLPPLT NGYPSSISVYETQTKYQSYNQYPNGSANGFGAVRNFSPTDYYHSEIPNTRPHEILEKPSPPQPPPPPSVP QTVIPKKTGSPEIKLKITKTIQNGRELFESSLCGDLLNEVQASEHTKSKHESRKEKRKKSNKHDSSRSEE RKSHKIPKLEPEEQNRPNERVDTVSEKPREEPVLKEEAPVQPILSSVPTTEVSTGVKFQVGDLVWSKVGT YPWWPCMVSSDPQLEVHTKINTRGAREYHVQFFSNQPERAWVHEKRVREYKGHKQYEELLAEATKQASNH SEKQKIRKPRPQRERAQWDIGIAHAEKALKMTREERIEQYTFIYIDKQPEEALSQAKKSVASKTEVKKTR RPRSVLNTQPEQTNAGEVASSLSSTEIRRHSQRRHTSAEEEEPPPVKIAWKTAAARKSLPASITMHKGSL DLQKCNMSPVVKIEQVFALQNATGDGKFIDQFVYSTKGIGNKTEISVRGQDRLIISTPNQRNEKPTQSVS SPEATSGSTGSVEKKQQRRSIRTRSESEKSTEVVPKKKIKKEQVETVPQATVKTGLQKGSADRGVQGSVR FSDSSVSAAIEETVD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017778 |
| ORF Size | 1935 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017778.2, NP_060248.2 |
| RefSeq Size | 3995 bp |
| RefSeq ORF | 1938 bp |
| Locus ID | 54904 |
| UniProt ID | Q9BZ95 |
| Domains | PWWP |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Lysine degradation |
| Gene Summary | This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. [provided by RefSeq, May 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212790 | WHSC1L1 (Myc-DDK-tagged)-Human Wolf-Hirschhorn syndrome candidate 1-like 1 (WHSC1L1), transcript variant short |
CNY 4,560.00 |
|
| RC212790L3 | Lenti ORF clone of Human Wolf-Hirschhorn syndrome candidate 1-like 1 (WHSC1L1), transcript variant short, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC212790L4 | Lenti ORF clone of Human Wolf-Hirschhorn syndrome candidate 1-like 1 (WHSC1L1), transcript variant short, mGFP tagged |
CNY 6,460.00 |
|
| SC111310 | WHSC1L1 (untagged)-Human Wolf-Hirschhorn syndrome candidate 1-like 1 (WHSC1L1), transcript variant short |
CNY 4,808.00 |
