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PIAS2 (NM_004671) Human Tagged ORF Clone
CAT＃: RG212659
- TrueORF®
PIAS2 (tGFP-tagged) - Human protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta
ORF Plasmid: DDK
"NM_004671" in other vectors (6)
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CNY 5,040.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARIP3; DIP; MIZ1; PIASX; SIZ2; ZMIZ4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212659 representing NM_004671
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATTTCGAAGAGTTGAGGAATATGGTTTCTAGTTTTAGGGTTTCTGAACTACAAGTATTACTAG GCTTTGCTGGACGGAATAAAAGTGGACGCAAGCATGACCTCCTGATGAGGGCGCTGCATTTATTGAAGAG CGGCTGCAGCCCTGCGGTTCAGATTAAAATCCGAGAATTGTATAGACGCCGATATCCACGAACTCTTGAA GGACTTTCTGATTTATCCACAATCAAATCATCGGTTTTCAGTTTGGATGGTGGCTCATCACCTGTAGAAC CTGACTTGGCCGTGGCTGGAATCCACTCGTTGCCTTCCACTTCAGTTACACCTCACTCACCATCCTCTCC TGTTGGTTCTGTGCTGCTTCAAGATACTAAGCCCACATTTGAGATGCAGCAGCCATCTCCCCCAATTCCT CCTGTCCATCCTGATGTGCAGTTAAAAAATCTGCCCTTTTATGATGTCCTTGATGTTCTCATCAAGCCCA CGAGTTTAGTTCAAAGCAGTATTCAGCGATTTCAAGAGAAGTTTTTTATTTTTGCTTTGACACCTCAACA AGTTAGAGAGATATGCATATCCAGGGATTTTTTGCCAGGTGGTAGGAGAGATTATACAGTCCAAGTTCAG TTGAGACTTTGCCTGGCAGAGACAAGTTGCCCTCAAGAAGATAACTATCCAAATAGTCTATGTATAAAAG TAAATGGGAAGCTATTTCCTTTGCCTGGCTATGCACCACCGCCTAAAAATGGGATTGAACAGAAGCGCCC TGGACGCCCCTTGAATATTACATCTTTAGTTAGGTTATCTTCAGCTGTGCCAAACCAAATTTCCATTTCT TGGGCATCAGAAATTGGGAAGAATTACTCTATGTCTGTATATCTTGTACGGCAGCTTACATCAGCCATGT TATTACAGAGATTAAAAATGAAAGGTATTAGAAACCCTGATCATTCCAGAGCACTAATTAAAGAAAAACT TACTGCAGATCCTGATAGTGAAATTGCTACAACTAGCCTTCGGGTATCCTTGATGTGCCCTTTAGGAAAA ATGAGGCTGACAATCCCATGCCGTGCAGTGACTTGTACACATCTGCAGTGTTTTGATGCTGCCCTCTATC TACAAATGAATGAGAAAAAGCCCACCTGGATTTGTCCTGTGTGTGACAAAAAAGCTGCCTATGAAAGTCT AATATTAGATGGGCTTTTTATGGAAATTCTCAATGACTGTTCTGATGTAGATGAGATCAAATTCCAAGAA GATGGTTCTTGGTGTCCAATGAGACCGAAGAAAGAAGCTATGAAAGTATCCAGCCAACCGTGTACAAAAA TAGAAAGTTCAAGCGTCCTCAGTAAGCCTTGTTCAGTGACTGTAGCCAGTGAGGCAAGCAAGAAGAAAGT AGATGTTATTGATCTTACAATAGAAAGCTCTTCTGACGAAGAGGAAGACCCTCCTGCCAAAAGGAAATGC ATCTTTATGTCAGAAACACAAAGCAGCCCAACCAAAGGGGTTCTCATGTATCAGCCATCTTCTGTAAGGG TGCCCAGTGTGACTTCGGTTGATCCTGCTGCTATTCCGCCTTCATTAACAGACTACTCAGTACCATTCCA CCATACGCCAATATCAAGCATGTCATCAGATTTGCCAGGTTTGGATTTTCTTTCCCTTATTCCAGTTGAT CCCCAGTACTGTCCTCCTATGTTTTTGGATAGTCTCACCTCACCCTTAACAGCAAGCAGTACGTCTGTCA CCACCACCAGCTCCCATGAAAGCAGTACTCATGTTAGTTCATCCAGCAGCAGGAGTGAGACAGGGGTCAT AACCAGCAGTGGAAGTAACATTCCTGACATCATCTCATTGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212659 representing NM_004671
Red=Cloning site Green=Tags(s) MADFEELRNMVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIRELYRRRYPRTLE GLSDLSTIKSSVFSLDGGSSPVEPDLAVAGIHSLPSTSVTPHSPSSPVGSVLLQDTKPTFEMQQPSPPIP PVHPDVQLKNLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQ LRLCLAETSCPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISIS WASEIGKNYSMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGK MRLTIPCRAVTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILNDCSDVDEIKFQE DGSWCPMRPKKEAMKVSSQPCTKIESSSVLSKPCSVTVASEASKKKVDVIDLTIESSSDEEEDPPAKRKC IFMSETQSSPTKGVLMYQPSSVRVPSVTSVDPAAIPPSLTDYSVPFHHTPISSMSSDLPGLDFLSLIPVD PQYCPPMFLDSLTSPLTASSTSVTTTSSHESSTHVSSSSSRSETGVITSSGSNIPDIISLD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004671 |
| ORF Size | 1863 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004671.5 |
| RefSeq Size | 2360 bp |
| RefSeq ORF | 1866 bp |
| Locus ID | 9063 |
| UniProt ID | O75928 |
| Domains | SAP, zf-MIZ |
| Protein Families | Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors |
| Protein Pathways | Jak-STAT signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis |
| Gene Summary | This gene encodes a member of the protein inhibitor of activated STAT family, which function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. [provided by RefSeq, Aug 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212659 | PIAS2 (Myc-DDK-tagged)-Human protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta |
CNY 4,560.00 |
|
| RC212659L1 | Lenti ORF clone of Human protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC212659L2 | Lenti ORF clone of Human protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta, mGFP tagged |
CNY 6,460.00 |
|
| RC212659L3 | Lenti ORF clone of Human protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC212659L4 | Lenti ORF clone of Human protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta, mGFP tagged |
CNY 6,460.00 |
|
| SC125358 | PIAS2 (untagged)-Human protein inhibitor of activated STAT, 2 (PIAS2), transcript variant beta |
CNY 4,632.00 |
