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ATP2B1 (NM_001682) Human Tagged ORF Clone
CAT＃: RG212619
- TrueORF®
ATP2B1 (tGFP-tagged) - Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 2. Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_001682" in other vectors (4)
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CNY 13,400.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PMCA1; PMCA1kb |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212619 ORF sequence, codon optimized.
Due to the complexity of NM_001682, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGACATGGCAAACAACTCAGTTGCTTACAGTGGTGTGAAAAACTCTTTGAAGGAAGCTAATCATG ATGGAGACTTTGGAATTACGCTCGCAGAGCTGCGGGCTCTCATGGAGCTCAGGTCCACAGATGCATTACG AAAAATACAGGAAAGCTATGGAGATGTCTATGGAATTTGCACCAAATTGAAAACATCTCCCAATGAAGGT TTAAGTGGAAACCCTGCAGATTTAGAAAGAAGAGAAGCAGTGTTTGGAAAGAATTTTATACCTCCTAAAA AGCCAAAAACCTTTCTTCAATTAGTATGGGAAGCATTACAAGATGTCACTTTAATTATATTAGAAATTGC AGCCATAGTATCATTGGGCCTTTCTTTTTATCAGCCTCCAGAAGGGGATAATGCACTTTGTGGAGAAGTT TCTGTTGGGGAGGAAGAAGGTGAAGGTGAAACTGGTTGGATTGAAGGAGCTGCAATCCTCTTGTCTGTAG TGTGTGTGGTGTTAGTAACAGCTTTCAATGACTGGAGTAAGGAAAAACAGTTTAGAGGTTTGCAGAGCCG AATTGAACAAGAACAGAAGTTCACTGTCATCAGGGGTGGTCAGGTCATTCAGATACCTGTAGCTGACATT ACTGTTGGAGATATTGCTCAAGTGAAATATGGTGATCTTCTTCCAGCTGACGGCATACTTATTCAAGGCA ACGATCTTAAAATTGATGAAAGCTCATTGACTGGTGAATCAGATCATGTTAAAAAGTCTTTAGATAAGGA TCCCTTACTTCTATCAGGTACTCATGTAATGGAAGGCTCTGGAAGAATGGTAGTTACAGCTGTAGGTGTA AATTCTCAAACTGGAATTATCTTTACCTTACTTGGAGCTGGAGGTGAAGAGGAAGAGAAGAAAGATGAGA AGAAAAAGGAAAAGAAAAATAAGAAACAAGATGGAGCTATTGAGAATCGCAACAAAGCAAAAGCCCAGGA TGGTGCAGCCATGGAAATGCAGCCATTGAAGAGTGAAGAAGGTGGAGATGGTGATGAAAAAGATAAAAAG AAAGCAAATTTGCCAAAAAAGGAAAAATCTGTTTTACAAGGGAAACTTACAAAACTGGCTGTTCAGATTG GCAAAGCAGGTCTGTTGATGTCTGCCATCACAGTTATCATTCTAGTATTATATTTTGTCATTGACACCTT CTGGGTTCAGAAAAGACCATGGCTTGCTGAGTGCACACCAATTTATATACAATACTTTGTGAAGTTCTTC ATTATTGGAGTTACAGTTTTAGTGGTCGCAGTGCCAGAAGGTCTTCCACTTGCAGTCACGATCTCACTGG CTTATTCAGTCAAAAAAATGATGAAAGATAATAACTTAGTAAGGCATCTGGATGCTTGTGAAACCATGGG AAATGCTACAGCTATTTGTTCAGATAAAACAGGAACTTTGACAATGAACAGAATGACAGTCGTTCAAGCT TACATAAATGAAAAACATTATAAAAAGGTTCCTGAACCAGAAGCTATTCCACCAAATATTTTGTCCTATC TTGTAACAGGAATTTCTGTGAATTGTGCTTATACATCAAAAATATTGCCACCAGAGAAAGAGGGTGGATT ACCTCGTCACGTTGGTAATAAAACTGAATGTGCCTTGTTGGGACTTCTTTTGGATTTAAAACGGGATTAT CAGGATGTTAGAAATGAAATACCAGAAGAAGCACTGTACAAAGTCTACACCTTCAATTCTGTTAGGAAGT CCATGAGTACTGTCCTGAAAAATTCAGATGGAAGTTATCGAATATTCAGCAAGGGTGCATCTGAGATAAT TCTGAAAAAGTGTTTCAAAATCTTGAGTGCTAATGGTGAGGCAAAAGTATTCAGACCAAGGGACCGTGAT GATATTGTAAAAACTGTGATTGAACCGATGGCATCAGAAGGCTTGAGAACCATATGTCTTGCATTCAGAG ATTTTCCAGCAGGAGAACCAGAACCAGAGTGGGATAATGAAAATGATATTGTCACCGGCCTTACATGCAT TGCTGTTGTGGGGATTGAAGATCCTGTGAGACCTGAGGTGCCAGATGCAATTAAAAAGTGTCAGAGGGCT GGAATTACTGTGCGGATGGTCACTGGTGATAATATTAATACTGCTCGGGCCATTGCTACCAAATGTGGTA TTTTACATCCTGGGGAAGATTTTCTGTGCCTAGAAGGTAAAGATTTTAACAGAAGAATACGAAATGAAAA AGGAGAGATTGAGCAAGAGAGGATAGACAAGATTTGGCCAAAACTTCGAGTACTTGCAAGATCATCTCCT ACTGATAAGCATACACTGGTTAAAGGTATAATTGACAGCACTGTCTCAGACCAACGCCAGGTTGTAGCTG TAACTGGTGATGGTACAAATGATGGCCCAGCACTAAAGAAAGCAGATGTTGGATTTGCAATGGGTATTGC TGGAACTGATGTAGCTAAAGAAGCATCCGATATTATTCTCACAGATGACAACTTTACAAGCATTGTTAAA GCAGTTATGTGGGGACGAAATGTCTATGACAGCATCTCAAAATTCCTTCAGTTCCAACTTACTGTTAATG TAGTAGCAGTGATTGTTGCTTTTACGGGCGCCTGCATTACTCAAGACTCACCGCTTAAGGCTGTGCAGAT GCTGTGGGTAAACCTCATAATGGATACACTCGCTTCCCTGGCTCTGGCAACGGAACCACCCACTGAGTCT CTCTTGCTTCGGAAACCTTATGGTAGAAATAAGCCTCTCATCTCACGTACAATGATGAAGAATATTTTGG GTCATGCATTCTATCAACTTGTAGTAGTCTTTACACTCTTATTTGCTGGAGAAAAGTTTTTTGACATTGA TAGTGGAAGAAATGCTCCTTTGCATGCTCCTCCTTCAGAACATTATACTATTGTTTTTAATACCTTTGTG CTGATGCAACTTTTCAACGAAATAAATGCCCGGAAAATTCATGGTGAAAGAAATGTATTCGAAGGAATCT TTAACAATGCCATCTTCTGCACAATTGTTTTAGGCACTTTTGTGGTACAGATAATAATTGTGCAGTTTGG TGGAAAACCTTTCAGTTGTTCAGAACTTTCAATAGAACAGTGGCTATGGTCAATATTCCTAGGAATGGGA ACATTACTCTGGGGCCAGCTTATTTCAACAATTCCAACTAGCCGTTTAAAATTCCTCAAAGAAGCTGGTC ATGGAACACAAAAGGAAGAAATACCTGAGGAGGAATTAGCAGAGGATGTTGAAGAGATTGATCACGCTGA AAGGGAGTTGCGGCGTGGCCAAATCTTGTGGTTTAGAGGTCTGAACAGAATCCAAACACAGATTCGAGTG GTGAATGCATTTCGTAGTTCTTTATATGAAGGGTTAGAAAAACCGGAATCAAGAAGTTCGATTCACAACT TTATGACACATCCTGAGTTTAGGATAGAAGATTCAGAGCCTCATATCCCCCTTATTGATGACACTGATGC CGAAGATGATGCTCCTACAAAACGTAACTCCAGTCCTCCACCCTCTCCCAACAAAAATAACAATGCTGTT GACAGTGGAATTCACCTTACAATAGAAATGAACAAGTCTGCTACCTCTTCATCCCCAGGAAGCCCACTAC ATAGTTTGGAAACATCACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212619 representing NM_001682
Red=Cloning site Green=Tags(s) MGDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTDALRKIQESYGDVYGICTKLKTSPNEG LSGNPADLERREAVFGKNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIVSLGLSFYQPPEGDNALCGEV SVGEEEGEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRGGQVIQIPVADI TVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPLLLSGTHVMEGSGRMVVTAVGV NSQTGIIFTLLGAGGEEEEKKDEKKKEKKNKKQDGAIENRNKAKAQDGAAMEMQPLKSEEGGDGDEKDKK KANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVLYFVIDTFWVQKRPWLAECTPIYIQYFVKFF IIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQA YINEKHYKKVPEPEAIPPNILSYLVTGISVNCAYTSKILPPEKEGGLPRHVGNKTECALLGLLLDLKRDY QDVRNEIPEEALYKVYTFNSVRKSMSTVLKNSDGSYRIFSKGASEIILKKCFKILSANGEAKVFRPRDRD DIVKTVIEPMASEGLRTICLAFRDFPAGEPEPEWDNENDIVTGLTCIAVVGIEDPVRPEVPDAIKKCQRA GITVRMVTGDNINTARAIATKCGILHPGEDFLCLEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSP TDKHTLVKGIIDSTVSDQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVK AVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTES LLLRKPYGRNKPLISRTMMKNILGHAFYQLVVVFTLLFAGEKFFDIDSGRNAPLHAPPSEHYTIVFNTFV LMQLFNEINARKIHGERNVFEGIFNNAIFCTIVLGTFVVQIIIVQFGGKPFSCSELSIEQWLWSIFLGMG TLLWGQLISTIPTSRLKFLKEAGHGTQKEEIPEEELAEDVEEIDHAERELRRGQILWFRGLNRIQTQIRV VNAFRSSLYEGLEKPESRSSIHNFMTHPEFRIEDSEPHIPLIDDTDAEDDAPTKRNSSPPPSPNKNNNAV DSGIHLTIEMNKSATSSSPGSPLHSLETSL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001682 |
| ORF Size | 3660 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001682.3 |
| RefSeq Size | 6967 bp |
| RefSeq ORF | 3663 bp |
| Locus ID | 490 |
| UniProt ID | P20020 |
| Domains | E1-E2_ATPase, Cation_ATPase_N, Hydrolase, Cation_ATPase_C |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Calcium signaling pathway |
| Gene Summary | The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212619 | ATP2B1 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 2. Note: ORF is codon optimized |
CNY 12,744.00 |
|
| RC212619L3 | Lenti-ORF clone of ATP2B1 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 2. Note: ORF is codon optimized |
CNY 14,060.00 |
|
| RC212619L4 | Lenti-ORF clone of ATP2B1 (mGFP-tagged)-Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 2. Note: ORF is codon optimized |
CNY 14,060.00 |
|
| SC309942 | ATP2B1 (untagged)-Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 2 |
CNY 23,560.00 |
