United States
Germany
Japan
United Kingdom
China
Dnmt2 (TRDMT1) (NM_176083) Human Tagged ORF Clone
CAT＃: RG212492
- TrueORF®
TRDMT1 (tGFP-tagged) - Human tRNA aspartic acid methyltransferase 1 (TRDMT1), transcript variant b
ORF Plasmid: DDK
"NM_176083" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DMNT2; DNMT2; M.HsaIIP; PuMet; RNMT1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212492 representing NM_176083
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCCTGCGGGTGCTGGAGCTATACAGCGGCGTGGGCGGCATGCACCACGCGCTGAGAGAAAGCT GTATACCTGCACAAGTGGTGGCTGCCATTGATGTCAACACTGTCGCTAATGAAGTATACAAGTATAATTT TCCTCACACACAGTTACTTGCCAAGACGATTGAAGGCATTACACTCGAAGAGTTTGACAGATTATCTTTT GATATGATTTTAATGAGCCCTCCCTGCCAGCCATTCACAAGATTACAAAAATTACCAAAGTATATTCTTT TGGAAAATGTTAAAGGTTTTGAAGTATCTTCTACAAGAGACCTCTTGATACAAACAATAGAAAATTGTGG CTTTCAGTACCAAGAGTTTCTATTATCTCCAACCTCTCTTGGCATTCCAAATTCAAGGCTACGATATTTT CTTATTGCAAAGCTTCAGTCAGAGCCATTACCCTTTCAAGCCCCTGGTCAGGTACTGATGGAGTTCCCCA AAATTGAATCTGTACATCCACAAAAATATGCAATGGATGTAGAAAATAAAATTCAAGAAAAGAACGTTGA ACCAAATATTAGCTTTGATGGCAGCATACAGTGTTCTGGAAAAGATGCCATTCTTTTTAAGCTTGAAACT GCAGAAGAAATTCACAGGAAAAATCAACAAGATAGTGATCTCTCTGTGAAAATGCTAAAAGATTTTCTTG AAGATGACACTGACGTGAACCAGTATCTTTTACCACCAAAGTCATTGCTGCGATATGCTCTTCTGTTAGA CATTGTTCAGCCCACTTGTAGAAGGTCCGTGTGCTTTACCAAAGGATATGGAAGCTACATAGAAGGGACA GGGTCTGTGTTACAGACTGCAGAGGATGTGCAGGTTGAGAATATCTACAAATCCCTTACCAATTTGTCAC AAGAAGAACAGATAACAAAGCTGTTAATACTTAAACTGCGATATTTCACTCCTAAAGAAATAGCAAATCT CCTTGGATTTCCTCCAGAGTTCGGATTTCCTGAGAAGATAACAGTGAAACAGCGTTATCGCCTACTTGGA AATAGTCTCAACGTGCATGTAGTAGCTAAACTAATCAAAATCTTATATGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212492 representing NM_176083
Red=Cloning site Green=Tags(s) MEPLRVLELYSGVGGMHHALRESCIPAQVVAAIDVNTVANEVYKYNFPHTQLLAKTIEGITLEEFDRLSF DMILMSPPCQPFTRLQKLPKYILLENVKGFEVSSTRDLLIQTIENCGFQYQEFLLSPTSLGIPNSRLRYF LIAKLQSEPLPFQAPGQVLMEFPKIESVHPQKYAMDVENKIQEKNVEPNISFDGSIQCSGKDAILFKLET AEEIHRKNQQDSDLSVKMLKDFLEDDTDVNQYLLPPKSLLRYALLLDIVQPTCRRSVCFTKGYGSYIEGT GSVLQTAEDVQVENIYKSLTNLSQEEQITKLLILKLRYFTPKEIANLLGFPPEFGFPEKITVKQRYRLLG NSLNVHVVAKLIKILYE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_176083 |
| ORF Size | 1101 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_176083.2, NP_788271.1 |
| RefSeq Size | 3467 bp |
| RefSeq ORF | 1103 bp |
| Locus ID | 1787 |
| Protein Families | Druggable Genome |
| Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways |
| Gene Summary | This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212492 | TRDMT1 (Myc-DDK-tagged)-Human tRNA aspartic acid methyltransferase 1 (TRDMT1), transcript variant b |
CNY 3,656.00 |
|
| RC212492L3 | Lenti ORF clone of Human tRNA aspartic acid methyltransferase 1 (TRDMT1), transcript variant b, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212492L4 | Lenti ORF clone of Human tRNA aspartic acid methyltransferase 1 (TRDMT1), transcript variant b, mGFP tagged |
CNY 5,890.00 |
|
| SC307060 | TRDMT1 (untagged)-Human tRNA aspartic acid methyltransferase 1 (TRDMT1), transcript variant b |
CNY 7,410.00 |
