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SPRED2 (NM_181784) Human Tagged ORF Clone
CAT＃: RG212199
- TrueORF®
SPRED2 (tGFP-tagged) - Human sprouty-related, EVH1 domain containing 2 (SPRED2), transcript variant 1
ORF Plasmid: DDK
"NM_181784" in other vectors (4)
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CNY 7,088.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Spred-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212199 representing NM_181784
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGAAGAAACACACCCAGACGATGACAGCTATATTGTGCGTGTCAAGGCTGTGGTTATGACCAGAG ATGACTCCAGCGGGGGATGGTTCCCACAGGAAGGAGGCGGGATCAGTCGCGTCGGGGTCTGTAAGGTCAT GCACCCCGAAGGCAATGGACGAAGCGGCTTTCTCATCCATGGTGAACGACAGAAAGACAAACTGGTGGTA TTGGAATGCTATGTAAGAAAGGACTTGGTCTACACCAAAGCCAATCCAACGTTTCATCACTGGAAGGTCG ATAATAGGAAGTTTGGACTTACTTTCCAAAGCCCTGCTGATGCCCGAGCCTTTGACAGGGGAGTAAGGAA AGCAATCGAAGACCTTATAGAAGGTTCAACAACGTCATCTTCCACCATCCATAATGAAGCTGAGCTTGGC GATGATGACGTTTTTACAACAGCTACAGACAGTTCTTCTAATTCCTCTCAGAAGAGAGAGCAACCTACTC GGACAATCTCCTCTCCCACATCCTGTGAGCACCGGAGGATTTATACCCTGGGCCACCTCCACGACTCATA CCCCACAGACCACTATCACCTCGATCAGCCGATGCCAAGGCCCTACCGCCAGGTGAGCTTCCCGGACGAC GACGAGGAGATCGTGCGCATCAACCCCCGGGAGAAGATCTGGATGACGGGGTACGAGGATTACCGGCACG CACCCGTCAGGGGCAAGTACCCGGACCCCTCGGAGGACGCGGACTCCTCCTACGTGCGCTTCGCCAAGGG CGAGGTCCCCAAGCATGACTACAACTACCCCTACGTGGACTCCTCAGACTTTGGCCTAGGCGAGGACCCC AAAGGCCGCGGGGGCAGCGTGATCAAGACGCAGCCCTCCCGGGGCAAGTCGCGGCGGCGGAAGGAGGACG GAGAGCGCTCGCGGTGCGTGTACTGCAGGGACATGTTCAACCACGAGGAGAACCGCCGGGGCCACTGCCA GGACGCGCCCGACTCCGTGAGAACTTGCATCCGCCGGGTGAGCTGCATGTGGTGCGCGGACAGCATGCTC TATCACTGTATGTCGGACCCCGAGGGAGACTATACAGACCCTTGCTCGTGCGATACTAGCGACGAGAAGT TTTGCCTCCGGTGGATGGCTCTTATTGCCTTGTCTTTCCTGGCCCCCTGTATGTGCTGTTACCTGCCCCT TCGGGCCTGCTACCACTGCGGAGTGATGTGCAGGTGCTGTGGCGGGAAGCACAAAGCGGCCGCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212199 representing NM_181784
Red=Cloning site Green=Tags(s) MTEETHPDDDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIHGERQKDKLVV LECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIEDLIEGSTTSSSTIHNEAELG DDDVFTTATDSSSNSSQKREQPTRTISSPTSCEHRRIYTLGHLHDSYPTDHYHLDQPMPRPYRQVSFPDD DEEIVRINPREKIWMTGYEDYRHAPVRGKYPDPSEDADSSYVRFAKGEVPKHDYNYPYVDSSDFGLGEDP KGRGGSVIKTQPSRGKSRRRKEDGERSRCVYCRDMFNHEENRRGHCQDAPDSVRTCIRRVSCMWCADSML YHCMSDPEGDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACYHCGVMCRCCGGKHKAAA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181784 |
| ORF Size | 1254 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_181784.3 |
| RefSeq Size | 4119 bp |
| RefSeq ORF | 1257 bp |
| Locus ID | 200734 |
| UniProt ID | Q7Z698 |
| Protein Pathways | Jak-STAT signaling pathway |
| Gene Summary | SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM, Mar 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Spred1 Safeguards Hematopoietic Homeostasis against Diet-Induced Systemic Stress.
,null,
Cell stem cell
,PubMed ID 29706577
[SPRED2]
|
|
Spred2 inhibits TGF-beta1-induced urokinase type plasminogen activator expression, cell motility and epithelial mesenchymal transition.
,null,
International journal of cancer
,PubMed ID 19908229
[SPRED2]
|
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212199 | SPRED2 (Myc-DDK-tagged)-Human sprouty-related, EVH1 domain containing 2 (SPRED2), transcript variant 1 |
CNY 5,488.00 |
|
| RC212199L3 | Lenti ORF clone of Human sprouty-related, EVH1 domain containing 2 (SPRED2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212199L4 | Lenti ORF clone of Human sprouty-related, EVH1 domain containing 2 (SPRED2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC313375 | SPRED2 (untagged)-Human sprouty-related, EVH1 domain containing 2 (SPRED2), transcript variant 1 |
CNY 5,488.00 |
