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DNALI1 (NM_003462) Human Tagged ORF Clone
CAT＃: RG212111
- TrueORF®
DNALI1 (tGFP-tagged) - Human dynein, axonemal, light intermediate chain 1 (DNALI1)
ORF Plasmid: DDK
"NM_003462" in other vectors (5)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | dJ423B22.5; hp28; P28 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212111 representing NM_003462
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGACGGCAAACAAGGCCCACACTGGACAGGGCAGCTGCTGGGTTGCTACTCTCGCCTCCGCCATGA TTCCGCCCGCAGACTCTTTGCTCAAGTACGACACCCCAGTGCTGGTGAGCCGGAACACGGAGAAACGGAG CCCCAAGGCTCGGCTACTGAAAGTCAGCCCCCAGCAGCCTGGACCTTCAGGTTCAGCCCCACAGCCACCC AAGACCAAGCTCCCCTCAACTCCCTGTGTCCCAGATCCTACAAAGCAGGCAGAAGAAATCTTGAATGCCA TACTACCCCCAAGGGAGTGGGTGGAAGACACGCAGCTATGGATCCAGCAGGTGTCCAGCACCCCTAGCAC CAGGATGGACGTGGTGCACCTCCAGGAGCAGTTAGACTTAAAGCTGCAGCAGCGGCAGGCCAGGGAAACA GGCATCTGCCCTGTCCGCAGGGAACTCTACTCACAGTGTTTTGATGAGTTGATCCGGGAGGTCACCATCA ACTGTGCGGAGAGGGGGCTGCTGCTGCTGCGAGTCCGGGACGAGATCCGCATGACCATCGCTGCCTACCA GACCCTGTACGAGAGCAGCGTGGCGTTTGGCATGAGGAAGGCACTGCAGGCTGAGCAGGGGAAGTCAGAC ATGGAGAGGAAAATCGCAGAATTGGAGACGGAAAAGAGAGACCTGGAGAGGCAAGTGAACGAGCAGAAGG CAAAATGTGAAGCCACTGAGAAGCGGGAGAGCGAGAGGCGGCAGGTGGAGGAGAAGAAGCACAATGAGGA GATTCAGTTCCTGAAGCGAACAAATCAGCAGCTGAAGGCCCAACTGGAAGGCATTATTGCACCAAAGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212111 representing NM_003462
Red=Cloning site Green=Tags(s) MVTANKAHTGQGSCWVATLASAMIPPADSLLKYDTPVLVSRNTEKRSPKARLLKVSPQQPGPSGSAPQPP KTKLPSTPCVPDPTKQAEEILNAILPPREWVEDTQLWIQQVSSTPSTRMDVVHLQEQLDLKLQQRQARET GICPVRRELYSQCFDELIREVTINCAERGLLLLRVRDEIRMTIAAYQTLYESSVAFGMRKALQAEQGKSD MERKIAELETEKRDLERQVNEQKAKCEATEKRESERRQVEEKKHNEEIQFLKRTNQQLKAQLEGIIAPKK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003462 |
| ORF Size | 840 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003462.3, NP_003453.2 |
| RefSeq Size | 2663 bp |
| RefSeq ORF | 777 bp |
| Locus ID | 7802 |
| UniProt ID | O14645 |
| Protein Pathways | Huntington's disease |
| Gene Summary | This gene is the human homolog of the Chlamydomonas inner dynein arm gene, p28. The precise function of this gene is not known, however, it is a potential candidate for immotile cilia syndrome (ICS). Ultrastructural defects of the inner dynein arms are seen in patients with ICS. Immotile mutant strains of Chlamydomonas, a biflagellated algae, exhibit similar defects. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212111 | DNALI1 (Myc-DDK-tagged)-Human dynein, axonemal, light intermediate chain 1 (DNALI1) |
CNY 2,400.00 |
|
| RC212111L3 | Lenti ORF clone of Human dynein, axonemal, light intermediate chain 1 (DNALI1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212111L4 | Lenti ORF clone of Human dynein, axonemal, light intermediate chain 1 (DNALI1), mGFP tagged |
CNY 5,890.00 |
|
| SC117948 | DNALI1 (untagged)-Human dynein, axonemal, light intermediate chain 1 (DNALI1) |
CNY 2,400.00 |
|
| SC317401 | DNALI1 (untagged)-Human dynein, axonemal, light intermediate chain 1 (DNALI1) |
CNY 6,270.00 |
