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NCKAP1L (NM_005337) Human Tagged ORF Clone
CAT＃: RG210832
- TrueORF®
NCKAP1L (tGFP-tagged) - Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1
ORF Plasmid: DDK
"NM_005337" in other vectors (5)
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CNY 12,350.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HEM1; IMD72 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG210832 representing NM_005337
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTTGACATCTGCTTACCAGCATAAATTAGCAGAGAAGCTCACTATCCTGAATGATCGCGGTCAGG GGGTTCTCATCCGTATGTATAACATCAAGAAGACTTGTTCAGACCCCAAATCTAAGCCACCTTTCTTACT GGAAAAGTCCATGGAACCATCTCTCAAGTATATCAACAAGAAATTTCCCAACATAGATGTCCGAAACAGC ACGCAACATTTAGGACCAGTACATCGTGAAAAAGCCGAGATAATTAGATTCCTCACCAACTACTACCAGT CATTTGTGGATGTCATGGAATTTCGGGATCATGTATATGAACTTCTCAACACCATTGATGCCTGCCAGTG CCATTTTGATATCAATCTCAACTTTGATTTCACTCGGAGTTACCTGGACTTGATTGTAACTTACACCTCA GTCATTTTACTTCTGTCACGGATTGAAGATCGGCGGATACTCATTGGCATGTACAATTGTGCCCATGAGA TGCTGCATGGGCATGGTGACCCCAGTTTTGCCCGTCTGGGTCAGATGGTCTTGGAGTATGACCACCCTCT GAAGAAGCTGACAGAAGAGTTTGGGCCTCACACAAAGGCTGTGAGTGGAGCCCTCCTCTCTTTGCATTTC CTCTTTGTCCGAAGAAACCAGGGGGCTGAGCAGTGGCGCAGTGCCCAACTTCTAAGCCTCATCAGCAACC CCCCAGCCATGATTAACCCTGCTAATTCAGATACAATGGCCTGTGAGTATCTGTCTGTGGAAGTAATGGA GCGCTGGATTATCATTGGGTTTCTTCTTTGTCATGGGTGCCTCAACTCCAATAGCCAGTGCCAGAAGCTG TGGAAGCTGTGTCTGCAGGGCTCCCTCTACATCACCCTTATCCGTGAGGATGTGCTGCAGGTGCACAAAG TCACCGAGGACCTGTTTAGCAGTTTGAAAGGGTATGGCAAGAGAGTGGCAGACATAAAGGAGAGCAAGGA ACATGTAATTGCAAACAGTGGCCAGTTTCATTGTCAACGGCGGCAATTTCTGCGGATGGCAGTGAAGGAG CTGGAGACTGTGTTGGCTGATGAACCGGGACTACTGGGTCCTAAGGCTCTTTTTGCTTTCATGGCCCTGT CCTTCATTCGTGATGAGGTCACCTGGCTGGTTCGCCACACAGAGAATGTCACCAAGACAAAGACACCTGA GGACTATGCTGACTCGAGCATTGCAGAGCTACTTTTCTTGTTGGAGGGGATTAGGTCTCTGGTCCGAAGA CACATCAAAGTGATACAGCAATACCACCTTCAGTACTTGGCAAGATTTGATGCTCTTGTGCTCAGTGACA TCATTCAGAACTTGTCTGTGTGTCCAGAGGAGGAGTCCATCATCATGTCCTCATTCGTCAGTATCCTCTC CTCTCTGAATCTCAAACAAGTTGATAATGGAGAAAAATTTGAATTCTCAGGATTGAGGCTGGACTGGTTC CGCCTACAGGCATACACTAGCGTGGCTAAGGCCCCTCTGCACCTGCATGAGAACCCTGACTTAGCCAAGG TGATGAACCTCATTGTCTTCCACTCCCGAATGCTGGACTCCGTAGAAAAATTGCTGGTGGAAACTTCTGA TCTGTCTACTTTCTGCTTTCATCTTCGTATCTTTGAGAAGATGTTTGCCATGACCTTGGAGGAATCTGCC ATGTTGCGTTATGCCATTGCTTTCCCCCTGATTTGTGCTCACTTTGTCCACTGCACTCATGAGATGTGCC CAGAGGAGTACCCCCACCTCAAGAACCATGGTCTTCACCACTGCAACTCCTTCCTGGAAGAGTTGGCCAA GCAGACCAGCAATTGCGTCCTGGAGATCTGTGCTGAGCAGCGAAACCTGAGCGAGCAGCTTCTACCTAAG CACTGTGCCACTACAATCAGCAAAGCCAAGAACAAGAAAACCAGGAAGCAGAGGCAGACTCCCAGAAAAG GAGAGCCCGAGAGGGACAAGCCAGGAGCTGAGAGTCACCGGAAGAACCGCAGCATTGTCACCAACATGGA CAAGCTACACCTAAACTTGACAGAACTGGCACTGACAATGAATCATGTATACAGTTTCTCCGTGTTTGAA CATACTATCTTCCCTTCTGAGTACCTCAGCAGCCACCTGGAGGCCAGACTCAACAGAGCCATTGTGTGGC TGGCTGGCTACAATGCCACGACCCAGGAGATCGTACGGCCTTCTGAGCTGTTGGCAGGAGTCAAAGCATA CATTGGTTTCATACAGTCACTGGCCCAGTTTTTGGGTGCAGATGCTTCCAGAGTCATCCGCAACGCCCTC CTGCAGCAGACACAACCACTGGATTCCTGTGGGGAACAGACAATCACCACACTCTACACAAACTGGTACC TGGAAAGTCTGCTTAGACAGGCAAGCAGTGGGACCATCATCCTCTCCCCAGCCATGCAGGCCTTCGTCAG CCTGCCCAGAGAAGGGGAGCAGAACTTCAGTGCAGAGGAGTTCTCTGACATCTCTGAGATGCGGGCCTTG GCAGAACTCCTGGGCCCCTATGGAATGAAGTTCCTGAGTGAAAACCTGATGTGGCATGTGACCTCTCAGA TTGTGGAGCTGAAGAAGCTGGTGGTGGAAAACATGGACATACTTGTTCAGATCAGATCCAACTTTAGCAA GCCGGACTTGATGGCTTCCCTGCTGCCCCAGCTGACAGGGGCTGAAAATGTGCTAAAGCGCATGACCATC ATTGGGGTTATCCTCAGTTTCAGGGCCATGGCCCAAGAGGGACTTCGGGAGGTTTTCTCCTCCCACTGCC CATTTCTTATGGGTCCCATTGAGTGCTTGAAGGAGTTTGTCACTCCAGACACAGACATCAAGGTGACCTT GAGTATCTTTGAGCTGGCATCTGCTGCAGGTGTGGGCTGTGACATTGACCCAGCCTTGGTGGCTGCCATT GCTAATCTGAAAGCTGATACTTCATCTCCTGAGGAGGAATATAAGGTGGCCTGCCTGCTCTTGATCTTTC TGGCAGTTTCCCTCCCACTCCTTGCCACTGACCCTTCTTCCTTTTATAGCATTGAGAAGGATGGTTACAA CAACAATATTCATTGCTTGACCAAAGCCATCATCCAGGTGTCTGCTGCCCTCTTCACGCTCTACAACAAG AACATTGAAACTCACCTCAAGGAATTTCTGGTGGTGGCCTCTGTCAGCCTCTTGCAGCTGGGCCAGGAGA CTGACAAGCTTAAAACCAGAAATCGAGAATCCATTTCTCTGCTCATGCGCTTGGTGGTGGAGGAGTCATC CTTCCTGACCCTGGACATGCTGGAGTCCTGTTTCCCTTATGTCCTGCTTCGAAATGCCTATCGGGAGGTG TCTCGGGCCTTCCACCTAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210832 representing NM_005337
Red=Cloning site Green=Tags(s) MSLTSAYQHKLAEKLTILNDRGQGVLIRMYNIKKTCSDPKSKPPFLLEKSMEPSLKYINKKFPNIDVRNS TQHLGPVHREKAEIIRFLTNYYQSFVDVMEFRDHVYELLNTIDACQCHFDINLNFDFTRSYLDLIVTYTS VILLLSRIEDRRILIGMYNCAHEMLHGHGDPSFARLGQMVLEYDHPLKKLTEEFGPHTKAVSGALLSLHF LFVRRNQGAEQWRSAQLLSLISNPPAMINPANSDTMACEYLSVEVMERWIIIGFLLCHGCLNSNSQCQKL WKLCLQGSLYITLIREDVLQVHKVTEDLFSSLKGYGKRVADIKESKEHVIANSGQFHCQRRQFLRMAVKE LETVLADEPGLLGPKALFAFMALSFIRDEVTWLVRHTENVTKTKTPEDYADSSIAELLFLLEGIRSLVRR HIKVIQQYHLQYLARFDALVLSDIIQNLSVCPEEESIIMSSFVSILSSLNLKQVDNGEKFEFSGLRLDWF RLQAYTSVAKAPLHLHENPDLAKVMNLIVFHSRMLDSVEKLLVETSDLSTFCFHLRIFEKMFAMTLEESA MLRYAIAFPLICAHFVHCTHEMCPEEYPHLKNHGLHHCNSFLEELAKQTSNCVLEICAEQRNLSEQLLPK HCATTISKAKNKKTRKQRQTPRKGEPERDKPGAESHRKNRSIVTNMDKLHLNLTELALTMNHVYSFSVFE HTIFPSEYLSSHLEARLNRAIVWLAGYNATTQEIVRPSELLAGVKAYIGFIQSLAQFLGADASRVIRNAL LQQTQPLDSCGEQTITTLYTNWYLESLLRQASSGTIILSPAMQAFVSLPREGEQNFSAEEFSDISEMRAL AELLGPYGMKFLSENLMWHVTSQIVELKKLVVENMDILVQIRSNFSKPDLMASLLPQLTGAENVLKRMTI IGVILSFRAMAQEGLREVFSSHCPFLMGPIECLKEFVTPDTDIKVTLSIFELASAAGVGCDIDPALVAAI ANLKADTSSPEEEYKVACLLLIFLAVSLPLLATDPSSFYSIEKDGYNNNIHCLTKAIIQVSAALFTLYNK NIETHLKEFLVVASVSLLQLGQETDKLKTRNRESISLLMRLVVEESSFLTLDMLESCFPYVLLRNAYREV SRAFHLN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005337 |
| ORF Size | 3381 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005337.3, NP_005328.2 |
| RefSeq Size | 3869 bp |
| RefSeq ORF | 3384 bp |
| Locus ID | 3071 |
| UniProt ID | P55160 |
| Protein Pathways | Regulation of actin cytoskeleton |
| Gene Summary | This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210832 | NCKAP1L (Myc-DDK-tagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1 |
CNY 11,210.00 |
|
| RC210832L3 | Lenti-ORF clone of NCKAP1L (Myc-DDK-tagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1 |
CNY 13,110.00 |
|
| RC210832L4 | Lenti-ORF clone of NCKAP1L (mGFP-tagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1 |
CNY 13,110.00 |
|
| SC116794 | NCKAP1L (untagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1 |
CNY 9,072.00 |
|
| SC318508 | NCKAP1L (untagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1 |
CNY 7,856.00 |
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