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C 4 Methylsterol Oxidase (MSMO1) (NM_006745) Human Tagged ORF Clone
CAT＃: RG210473
- TrueORF®
MSMO1 (tGFP-tagged) - Human sterol-C4-methyl oxidase-like (SC4MOL), transcript variant 1
ORF Plasmid: DDK
"NM_006745" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DESP4; ERG25; MCCPD; SC4MOL |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG210473 representing NM_006745
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAACAAATGAAAGTGTCAGCATCTTTAGTTCAGCATCCTTGGCTGTGGAATATGTAGATTCACTTT TACCTGAGAATCCTCTGCAAGAACCATTTAAAAATGCTTGGAACTATATGTTGAATAATTATACAAAGTT CCAGATTGCAACATGGGGATCCCTTATAGTTCATGAAGCCCTTTATTTCTTATTCTGTTTACCTGGATTT TTATTTCAATTTATACCTTATATGAAAAAATACAAAATTCAAAAGGATAAGCCAGAGACATGGGAAAACC AATGGAAGTGTTTCAAAGTTCTTCTCTTTAATCACTTCTGTATCCAGCTGCCTTTGATTTGTGGAACCTA TTATTTTACAGAGTATTTCAATATTCCTTATGATTGGGAAAGAATGCCAAGATGGTATTTTCTTTTGGCA AGATGCTTTGGTTGTGCAGTCATTGAAGATACTTGGCACTATTTTCTGCATAGACTCTTACACCACAAAA GAATATACAAGTATATTCATAAAGTTCATCATGAGTTTCAGGCTCCATTTGGAATGGAAGCTGAATATGC ACATCCTTTGGAGACTCTAATTCTTGGAACTGGATTTTTCATTGGAATCGTGCTTTTGTGTGATCATGTA ATTCTTCTTTGGGCATGGGTGACCATTCGTTTATTAGAAACTATTGATGTCCATAGTGGTTATGATATTC CTCTCAACCCTTTAAATCTGATCCCTTTCTATGCTGGTTCTCGGCATCATGATTTCCACCACATGAACTT CATTGGAAACTATGCTTCAACATTTACATGGTGGGATCGAATTTTTGGAACAGACTCTCAGTATAATGCC TATAATGAAAAGAGGAAGAAGTTTGAGAAAAAGACTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210473 representing NM_006745
Red=Cloning site Green=Tags(s) MATNESVSIFSSASLAVEYVDSLLPENPLQEPFKNAWNYMLNNYTKFQIATWGSLIVHEALYFLFCLPGF LFQFIPYMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWYFLLA RCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHV ILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFIGNYASTFTWWDRIFGTDSQYNA YNEKRKKFEKKTE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006745 |
| ORF Size | 879 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006745.5 |
| RefSeq Size | 2214 bp |
| RefSeq ORF | 882 bp |
| Locus ID | 6307 |
| UniProt ID | Q15800 |
| Domains | Sterol_desat |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, Steroid biosynthesis |
| Gene Summary | Sterol-C4-mehtyl oxidase-like protein was isolated based on its similarity to the yeast ERG25 protein. It contains a set of putative metal binding motifs with similarity to that seen in a family of membrane desaturases-hydroxylases. The protein is localized to the endoplasmic reticulum membrane and is believed to function in cholesterol biosynthesis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210473 | MSMO1 (Myc-DDK-tagged)-Human sterol-C4-methyl oxidase-like (SC4MOL), transcript variant 1 |
CNY 2,400.00 |
|
| RC210473L1 | Lenti ORF clone of Human sterol-C4-methyl oxidase-like (SC4MOL), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC210473L2 | Lenti ORF clone of Human sterol-C4-methyl oxidase-like (SC4MOL), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC210473L3 | Lenti ORF clone of Human sterol-C4-methyl oxidase-like (SC4MOL), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210473L4 | Lenti ORF clone of Human sterol-C4-methyl oxidase-like (SC4MOL), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC319845 | MSMO1 (untagged)-Human sterol-C4-methyl oxidase-like (SC4MOL), transcript variant 1 |
CNY 2,400.00 |
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