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UGT8 (NM_003360) Human Tagged ORF Clone
CAT＃: RG210442
- TrueORF®
UGT8 (tGFP-tagged) - Human UDP glycosyltransferase 8 (UGT8), transcript variant 2
ORF Plasmid: DDK
"NM_003360" in other vectors (6)
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CNY 4,370.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CGT; UGT4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG210442 representing NM_003360
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTCTTACACTCCATATTTCATTCTCCTGTGGAGTGCTGTTGGGATAGCGAAGGCTGCCAAAATCA TCATCGTGCCGCCAATTATGTTTGAAAGCCATATGTACATTTTCAAGACGCTAGCCTCAGCCTTGCACGA GAGAGGCCACCATACAGTGTTCCTCCTCTCTGAAGGCAGAGACATCGCCCCATCTAATCATTACAGCCTC CAGCGCTACCCAGGGATCTTTAACAGTACCACCTCAGATGCTTTCCTACAGTCCAAGATGCGGAATATTT TCTCTGGGAGATTGACAGCAATCGAACTGTTTGACATACTGGATCACTATACTAAGAACTGTGACCTGAT GGTTGGCAACCATGCCCTGATCCAGGGTCTGAAGAAAGAAAAATTTGACCTGCTGCTGGTGGACCCTAAT GATATGTGTGGATTTGTGATAGCTCATCTTTTAGGGGTTAAATATGCTGTATTTTCAACTGGCCTTTGGT ATCCTGCTGAAGTGGGTGCTCCTGCTCCATTAGCATACGTCCCAGAGTTTAACTCACTCCTCACAGACCG CATGAACTTGCTGCAAAGGATGAAAAATACCGGTGTTTACCTCATTTCCAGATTAGGGGTCAGCTTTCTG GTTCTTCCCAAATATGAAAGGATAATGCAGAAGTACAACCTGCTGCCGGAGAAGTCCATGTATGATTTGG TTCATGGGTCCAGCCTGTGGATGCTGTGTACTGACGTAGCACTGGAATTCCCAAGACCCACTCTGCCTAA TGTTGTTTATGTAGGAGGAATCCTAACCAAACCAGCCAGCCCACTACCAGAAGATCTCCAAAGATGGGTA AATGGTGCTAATGAACATGGCTTTGTCTTGGTGTCTTTTGGAGCTGGTGTCAAGTATCTGTCAGAAGACA TTGCTAACAAACTGGCAGGAGCTCTGGGGAGATTGCCTCAAAAAGTGATTTGGAGGTTTTCTGGACCCAA ACCAAAGAATCTAGGAAACAACACTAAACTCATAGAATGGTTACCACAAAATGACCTGCTTGGGCATTCA AAGATTAAAGCCTTCCTGAGCCATGGTGGTTTGAACAGTATTTTTGAAACTATGTATCATGGTGTGCCTG TAGTGGGAATTCCACTCTTTGGAGACCATTATGATACTATGACCAGAGTACAGGCAAAAGGCATGGGGAT ATTGCTAGAATGGAAGACAGTTACTGAAAAAGAGCTCTATGAAGCACTAGTGAAGGTTATCAATAATCCC AGCTACCGTCAGAGGGCTCAGAAGCTTTCGGAAATTCACAAGGATCAACCTGGTCACCCTGTCAATCGAA CTATCTATTGGATAGATTATATTATTCGTCACAATGGAGCCCATCACCTACGTGCCGCTGTCCATCAGAT CTCCTTTTGTCAGTATTTTTTACTGGATATTGCCTTTGTGCTTTTGCTTGGTGCTGCCTTGTTATACTTT CTCTTGTCTTGGGTGACAAAATTTATCTACAGAAAAATCAAAAGTCTGTGGTCTAGAAATAAGCATAGCA CAGTTAATGGACATTACCACAATGGAATCCTCAATGGCAAGTACAAAAGAAATGGCCATATTAAACATGA AAAGAAAGTGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210442 representing NM_003360
Red=Cloning site Green=Tags(s) MKSYTPYFILLWSAVGIAKAAKIIIVPPIMFESHMYIFKTLASALHERGHHTVFLLSEGRDIAPSNHYSL QRYPGIFNSTTSDAFLQSKMRNIFSGRLTAIELFDILDHYTKNCDLMVGNHALIQGLKKEKFDLLLVDPN DMCGFVIAHLLGVKYAVFSTGLWYPAEVGAPAPLAYVPEFNSLLTDRMNLLQRMKNTGVYLISRLGVSFL VLPKYERIMQKYNLLPEKSMYDLVHGSSLWMLCTDVALEFPRPTLPNVVYVGGILTKPASPLPEDLQRWV NGANEHGFVLVSFGAGVKYLSEDIANKLAGALGRLPQKVIWRFSGPKPKNLGNNTKLIEWLPQNDLLGHS KIKAFLSHGGLNSIFETMYHGVPVVGIPLFGDHYDTMTRVQAKGMGILLEWKTVTEKELYEALVKVINNP SYRQRAQKLSEIHKDQPGHPVNRTIYWIDYIIRHNGAHHLRAAVHQISFCQYFLLDIAFVLLLGAALLYF LLSWVTKFIYRKIKSLWSRNKHSTVNGHYHNGILNGKYKRNGHIKHEKKVK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003360 |
| ORF Size | 1623 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003360.4 |
| RefSeq Size | 2448 bp |
| RefSeq ORF | 1626 bp |
| Locus ID | 7368 |
| UniProt ID | Q16880 |
| Domains | UDPGT |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
| Gene Summary | The protein encoded by this gene belongs to the UDP-glycosyltransferase family. It catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central and peripheral nervous systems. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Microbubble-based enhancement of radiation effect: Role of cell membrane ceramide metabolism
,Al-Mahrouki, A;Giles, A;Hashim, A;Kim, HC;El-Falou, A;Rowe-Magnus, D;Farhat, G;Czarnota, GJ;,
PLoS ONE
,PubMed ID 28746357
[UGT8]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210442 | UGT8 (Myc-DDK-tagged)-Human UDP glycosyltransferase 8 (UGT8), transcript variant 2 |
CNY 6,040.00 |
|
| RC210442L1 | Lenti-ORF clone of UGT8 (Myc-DDK-tagged)-Human UDP glycosyltransferase 8 (UGT8), transcript variant 2 |
CNY 8,440.00 |
|
| RC210442L2 | Lenti-ORF clone of UGT8 (mGFP-tagged)-Human UDP glycosyltransferase 8 (UGT8), transcript variant 2 |
CNY 5,890.00 |
|
| RC210442L3 | Lenti-ORF clone of UGT8 (Myc-DDK-tagged)-Human UDP glycosyltransferase 8 (UGT8), transcript variant 2 |
CNY 5,890.00 |
|
| RC210442L4 | Lenti-ORF clone of UGT8 (mGFP-tagged)-Human UDP glycosyltransferase 8 (UGT8), transcript variant 2 |
CNY 5,890.00 |
|
| SC118055 | UGT8 (untagged)-Human UDP glycosyltransferase 8 (UGT8), transcript variant 2 |
CNY 2,950.00 |
