United States
Germany
Japan
United Kingdom
China
MLC1 (NM_015166) Human Tagged ORF Clone
CAT＃: RG209731
- TrueORF®
MLC1 (tGFP-tagged) - Human megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 1
ORF Plasmid: DDK
"NM_015166" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | LVM; MLC; VL |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG209731 representing NM_015166
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCAGGAGCCATTCAGAGAGGAGCTAGCCTATGACCGGATGCCCACGCTGGAGCGGGGCCGGCAAG ACCCCGCCAGCTATGCCCCAGACGCGAAGCCGAGCGACCTGCAGCTGTCGAAGAGACTGCCCCCCTGCTT CAGCCACAAGACGTGGGTCTTCTCTGTGCTGATGGGGAGCTGCCTCCTGGTGACCTCGGGGTTTTCGCTG TACCTGGGGAACGTGTTCCCGGCTGAGATGGATTACTTGCGCTGTGCTGCAGGCTCTTGCATCCCCTCGG CAATTGTGAGCTTCACCGTCTCCAGGAGGAACGCCAATGTGATTCCCAACTTTCAGATATTGTTTGTTTC CACGTTTGCTGTGACCACTACGTGTTTAATTTGGTTTGGATGCAAACTAGTCCTGAACCCATCAGCAATA AACATCAACTTCAACCTCATCCTGCTGCTCCTGCTGGAGCTGCTCATGGCGGCCACGGTGATCATCGCTG CACGGTCCAGCGAGGAGGACTGCAAGAAAAAGAAGGGCTCCATGTCTGACAGCGCCAACATTCTGGACGA AGTGCCATTTCCTGCTCGGGTCCTGAAATCTTACTCAGTCGTCGAGGTAATCGCAGGCATCTCTGCCGTC CTCGGGGGGATCATTGCCCTGAACGTGGATGACTCAGTTTCAGGCCCACACCTCTCAGTGACGTTCTTTT GGATCCTAGTGGCCTGCTTTCCAAGTGCCATTGCCAGTCATGTGGCAGCAGAGTGTCACAGCAAGTGTCT GGTGGAGGTCCTGATTGCCATAAGCAGCCTCACGTCTCCGCTGCTGTTCACAGCCTCTGGATATCTGTCA TTCAGCATCATGAGAATCGTGGAGATGTTTAAGGATTACCCGCCAGCCATAAAACCATCCTACGATGTGC TGCTGCTGCTGCTGCTGCTAGTGCTCCTGCTGCAGGCCGGCCTCAACACGGGCACCGCCATCCAGTGCGT GCGCTTCAAGGTCAGTGCAAGGCTGCAGGGTGCATCCTGGGACACCCAGAACGGCCCGCAGGAGCGCCTG GCTGGGGAGGTGGCCAGGAGCCCCCTGAAGGAGTTCGACAAGGAGAAAGCCTGGAGAGCCGTCGTGGTGC AAATGGCCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209731 representing NM_015166
Red=Cloning site Green=Tags(s) MTQEPFREELAYDRMPTLERGRQDPASYAPDAKPSDLQLSKRLPPCFSHKTWVFSVLMGSCLLVTSGFSL YLGNVFPAEMDYLRCAAGSCIPSAIVSFTVSRRNANVIPNFQILFVSTFAVTTTCLIWFGCKLVLNPSAI NINFNLILLLLLELLMAATVIIAARSSEEDCKKKKGSMSDSANILDEVPFPARVLKSYSVVEVIAGISAV LGGIIALNVDDSVSGPHLSVTFFWILVACFPSAIASHVAAECHSKCLVEVLIAISSLTSPLLFTASGYLS FSIMRIVEMFKDYPPAIKPSYDVLLLLLLLVLLLQAGLNTGTAIQCVRFKVSARLQGASWDTQNGPQERL AGEVARSPLKEFDKEKAWRAVVVQMAQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015166 |
| ORF Size | 1131 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015166.3, NP_055981.1 |
| RefSeq Size | 3960 bp |
| RefSeq ORF | 1134 bp |
| Locus ID | 23209 |
| UniProt ID | Q15049 |
| Protein Families | Ion Channels: Other, Transmembrane |
| Gene Summary | The function of this gene product is unknown; however, homology to other proteins suggests that it may be an integral membrane transporter. Mutations in this gene have been associated with megalencephalic leukoencephalopathy with subcortical cysts, an autosomal recessive neurological disorder. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209731 | MLC1 (Myc-DDK-tagged)-Human megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 1 |
CNY 3,656.00 |
|
| RC209731L3 | Lenti ORF clone of Human megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209731L4 | Lenti ORF clone of Human megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC102570 | MLC1 (untagged)-Human megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1), transcript variant 1 |
CNY 2,950.00 |
