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GPCR MRGX2 (MRGPRX2) (NM_054030) Human Tagged ORF Clone
CAT＃: RG209033
- TrueORF®
MRGPRX2 (tGFP-tagged) - Human MAS-related GPR, member X2 (MRGPRX2)
ORF Plasmid: DDK
"NM_054030" in other vectors (4)
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CNY 4,000.00
CNY 4,370.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGRG3; MRGX2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG209033 representing NM_054030
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCAACCACCCCGGCCTGGGGAACAGAAAGTACAACAGTGAATGGAAATGACCAAGCCCTTCTTC TGCTTTGTGGCAAGGAGACCCTGATCCCGGTCTTCCTGATCCTTTTCATTGCCCTGGTCGGGCTGGTAGG AAACGGGTTTGTGCTCTGGCTCCTGGGCTTCCGCATGCGCAGGAACGCCTTCTCTGTCTACGTCCTCAGC CTGGCCGGGGCCGACTTCCTCTTCCTCTGCTTCCAGATTATAAATTGCCTGGTGTACCTCAGTAACTTCT TCTGTTCCATCTCCATCAATTTCCCTAGCTTCTTCACCACTGTGATGACCTGTGCCTACCTTGCAGGCCT GAGCATGCTGAGCACCGTCAGCACCGAGCGCTGCCTGTCCGTCCTGTGGCCCATCTGGTATCGCTGCCGC CGCCCCAGACACCTGTCAGCGGTCGTGTGTGTCCTGCTCTGGGCCCTGTCCCTACTGCTGAGCATCTTGG AAGGGAAGTTCTGTGGCTTCTTATTTAGTGATGGTGACTCTGGTTGGTGTCAGACATTTGATTTCATCAC TGCAGCGTGGCTGATTTTTTTATTCATGGTTCTCTGTGGGTCCAGTCTGGCCCTGCTGGTCAGGATCCTC TGTGGCTCCAGGGGTCTGCCACTGACCAGGCTGTACCTGACCATCCTGCTCACAGTGCTGGTGTTCCTCC TCTGCGGCCTGCCCTTTGGCATTCAGTGGTTCCTAATATTATGGATCTGGAAGGATTCTGATGTCTTATT TTGTCATATTCATCCAGTTTCAGTTGTCCTGTCATCTCTTAACAGCAGTGCCAACCCCATCATTTACTTC TTCGTGGGCTCTTTTAGGAAGCAGTGGCGGCTGCAGCAGCCGATCCTCAAGCTGGCTCTCCAGAGGGCTC TGCAGGACATTGCTGAGGTGGATCACAGTGAAGGATGCTTCCGTCAGGGCACCCCGGAGATGTCGAGAAG CAGTCTGGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209033 representing NM_054030
Red=Cloning site Green=Tags(s) MDPTTPAWGTESTTVNGNDQALLLLCGKETLIPVFLILFIALVGLVGNGFVLWLLGFRMRRNAFSVYVLS LAGADFLFLCFQIINCLVYLSNFFCSISINFPSFFTTVMTCAYLAGLSMLSTVSTERCLSVLWPIWYRCR RPRHLSAVVCVLLWALSLLLSILEGKFCGFLFSDGDSGWCQTFDFITAAWLIFLFMVLCGSSLALLVRIL CGSRGLPLTRLYLTILLTVLVFLLCGLPFGIQWFLILWIWKDSDVLFCHIHPVSVVLSSLNSSANPIIYF FVGSFRKQWRLQQPILKLALQRALQDIAEVDHSEGCFRQGTPEMSRSSLV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_054030 |
| ORF Size | 990 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_054030.4 |
| RefSeq Size | 2130 bp |
| RefSeq ORF | 993 bp |
| Locus ID | 117194 |
| UniProt ID | Q96LB1 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Gene Summary | Mast cell-specific receptor for basic secretagogues, i.e. cationic amphiphilic drugs, as well as endo- or exogenous peptides, consisting of a basic head group and a hydrophobic core (PubMed:25517090). Recognizes and binds small molecules containing a cyclized tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular blocking drugs (NMBDs), including tubocurarine and atracurium. In response to these compounds, mediates pseudo-allergic reactions characterized by histamine release, inflammation and airway contraction (By similarity). Acts as a receptor for a number of other ligands, including peptides and alkaloids, such as cortistatin-14, proadrenomedullin N-terminal peptides PAMP-12 and, at lower extent, PAMP-20, antibacterial protein LL-37, PMX-53 peptide, beta-defensins, and complanadine A.[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A novel MRGPRX2-targeting antagonistic DNA aptamer inhibits histamine release and prevents mast cell-mediated anaphylaxis
,Suzuki, Y;Liu, S;Ogasawara, T;Sawasaki, T;Takasaki, Y;Yorozuya, T;Mogi, M;,
Eur. J. Pharmacol.
,PubMed ID 32320700
[MRGPRX2]
|
|
Tachykinin-1 receptor antagonism suppresses substance-P- and compound 48/80-induced mast cell activation from rat mast cells expressing functional mas-related GPCR B3
,Sahid, MNA;Liu, S;Mogi, M;Maeyama, K;,
Inflamm. Res.
,PubMed ID 31993675
[MRGPRX2]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209033 | MRGPRX2 (Myc-DDK-tagged)-Human MAS-related GPR, member X2 (MRGPRX2) |
CNY 2,400.00 |
|
| RC209033L3 | Lenti ORF clone of Human MAS-related GPR, member X2 (MRGPRX2), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC209033L4 | Lenti ORF clone of Human MAS-related GPR, member X2 (MRGPRX2), mGFP tagged |
CNY 4,800.00 |
|
| SC120221 | MRGPRX2 (untagged)-Human MAS-related GPR, member X2 (MRGPRX2) |
CNY 2,400.00 |
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