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MID1 (NM_000381) Human Tagged ORF Clone
CAT＃: RG208776
- TrueORF®
MID1 (tGFP-tagged) - Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1
ORF Plasmid: DDK
"NM_000381" in other vectors (7)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BBBG1; FXY; GBBB1; MIDIN; OGS1; OS; OSX; RNF59; TRIM18; XPRF; ZNFXY |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208776 representing NM_000381
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACACTGGAGTCAGAACTGACCTGCCCTATTTGTCTGGAGCTCTTTGAGGACCCTCTTCTACTGC CCTGCGCACACAGCCTCTGCTTCAACTGCGCCCACCGCATCCTAGTATCACACTGTGCCACCAACGAGTC TGTGGAGTCCATCACCGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACCCTCAGCCAGCGAGGTCTA GACGGGCTCAAGCGCAACGTCACCCTACAGAACATCATCGACAGGTTCCAGAAAGCATCAGTGAGCGGGC CCAACTCTCCCAGCGAGACCCGTCGGGAGCGGGCCTTTGACGCCAACACCATGACCTCCGCCGAGAAGGT CCTCTGCCAGTTTTGTGACCAGGATCCTGCCCAGGACGCTGTGAAGACCTGTGTCACTTGTGAAGTATCC TACTGTGACGAGTGCCTGAAAGCCACTCACCCGAATAAGAAGCCCTTTACAGGCCATCGTCTGATTGAGC CAATTCCGGACTCTCACATCCGGGGGCTGATGTGCTTGGAGCATGAGGATGAGAAGGTGAATATGTACTG TGTGACCGATGACCAGTTAATCTGTGCCTTGTGTAAACTGGTTGGGCGGCACCGCGATCATCAGGTGGCA GCTTTGAGTGAGCGCTATGACAAATTGAAGCAAAACTTAGAGAGTAACCTCACCAACCTTATTAAGAGGA ACACAGAACTGGAGACCCTTTTGGCTAAACTCATCCAAACCTGTCAACATGTTGAAGTCAATGCATCACG TCAAGAAGCCAAATTGACAGAGGAGTGTGATCTTCTCATTGAGATCATTCAGCAAAGACGACAGATTATT GGAACCAAGATCAAAGAAGGGAAGGTGATGAGGCTTCGCAAACTGGCTCAGCAGATTGCAAACTGCAAAC AGTGCATTGAGCGGTCAGCATCACTCATCTCCCAAGCGGAACACTCTCTGAAGGAGAATGATCATGCGCG TTTCCTACAGACTGCTAAGAATATCACCGAGAGAGTCTCCATGGCAACTGCATCCTCCCAGGTTCTAATT CCTGAAATCAACCTCAATGACACATTTGACACCTTTGCCTTAGATTTTTCCCGAGAGAAGAAACTGCTAG AATGTCTGGATTACCTTACAGCTCCCAACCCTCCCACAATTAGAGAAGAGCTCTGCACAGCTTCATATGA CACCATCACTGTGCATTGGACCTCCGATGATGAGTTCAGCGTGGTCTCCTACGAGCTCCAGTACACCATA TTCACCGGACAAGCCAACGTCGTTAGTCTGTGTAATTCGGCTGATAGCTGGATGATAGTACCCAACATCA AGCAGAACCACTACACGGTGCACGGTCTGCAGAGCGGCACCAAGTACATCTTCATGGTCAAGGCCATCAA CCAGGCGGGCAGCCGCAGCAGTGAGCCTGGGAAGTTGAAGACAAACAGCCAACCATTTAAACTGGATCCC AAATCTGCTCATCGAAAACTGAAGGTGTCCCATGATAACTTGACAGTAGAACGTGATGAGTCATCATCCA AGAAGAGTCACACACCTGAACGCTTCACCAGCCAGGGGAGCTATGGAGTAGCTGGAAATGTGTTTATTGA TAGTGGCCGGCATTATTGGGAAGTGGTCATAAGTGGAAGCACATGGTATGCCATTGGTCTTGCTTACAAA TCAGCCCCGAAGCATGAATGGATTGGGAAGAACTCTGCTTCCTGGGCGCTCTGCCGCTGCAACAATAACT GGGTGGTGAGACACAATAGCAAGGAAATCCCCATTGAGCCTGCCCCCCACCTCCGGCGCGTGGGCATCCT GCTGGACTATGATAACGGCTCTATCGCCTTTTATGATGCTTTGAACTCCATCCACCTCTACACCTTCGAC GTCGCATTTGCGCAGCCTGTTTGCCCCACCTTCACCGTGTGGAACAAGTGTCTGACGATTATCACTGGGC TCCCTATCCCAGACCATTTGGACTGCACAGAGCAGCTGCCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208776 representing NM_000381
Red=Cloning site Green=Tags(s) METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCATNESVESITAFQCPTCRHVITLSQRGL DGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAFDANTMTSAEKVLCQFCDQDPAQDAVKTCVTCEVS YCDECLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVA ALSERYDKLKQNLESNLTNLIKRNTELETLLAKLIQTCQHVEVNASRQEAKLTEECDLLIEIIQQRRQII GTKIKEGKVMRLRKLAQQIANCKQCIERSASLISQAEHSLKENDHARFLQTAKNITERVSMATASSQVLI PEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPTIREELCTASYDTITVHWTSDDEFSVVSYELQYTI FTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFMVKAINQAGSRSSEPGKLKTNSQPFKLDP KSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYK SAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFD VAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000381 |
| ORF Size | 2001 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000381.4 |
| RefSeq Size | 3452 bp |
| RefSeq ORF | 2004 bp |
| Locus ID | 4281 |
| UniProt ID | O15344 |
| Domains | zf-B_box, RING, BBC, SPRY, FN3 |
| Protein Families | Druggable Genome |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208776 | MID1 (Myc-DDK-tagged)-Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1 |
CNY 3,990.00 |
|
| RC208776L1 | Lenti ORF clone of Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208776L2 | Lenti ORF clone of Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC208776L3 | Lenti ORF clone of Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208776L4 | Lenti ORF clone of Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC109438 | MID1 (untagged)-Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1 |
CNY 2,950.00 |
|
| SC324026 | MID1 (untagged)-Human midline 1 (Opitz/BBB syndrome) (MID1), transcript variant 1 |
CNY 2,950.00 |
