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TAF5 (NM_006951) Human Tagged ORF Clone
CAT＃: RG208552
- TrueORF®
TAF5 (tGFP-tagged) - Human TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa (TAF5)
ORF Plasmid: DDK
"NM_006951" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | TAF(II)100; TAF2D; TAFII-100; TAFII100 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208552 representing NM_006951
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCTGGCGGAGGAGCAGACGGAGGTGGCGGTCAAGCTAGAGCCTGAGGGACCGCCAACGCTGC TACCTCCGCAGGCGGGGGACGGCGCAGGCGAGGGTAGCGGCGGCACTACCAACAACGGCCCCAACGGCGG CGGCGGGAACGTTGCGGCGTCGTCGTCCACTGGCGGGGATGGCGGGACCCCCAAGCCCACGGTGGCTGTC TCCGCCGCTGCCCCGGCGGGGGCGGCCCCGGTGCCCGCCGCTGCTCCGGACGCCGGCGCTCCGCATGACC GACAGACTCTACTGGCCGTGCTGCAGTTCCTACGGCAGAGCAAACTCCGCGAGGCCGAAGAGGCGCTGCG CCGTGAGGCCGGGCTGCTGGAGGAGGCAGTGGCGGGCTCCGGAGCCCCGGGAGAGGTGGACAGCGCCGGC GCTGAGGTGACCAGCGCGCTTCTCAGCCGGGTGACCGCCTCGGCCCCTGGCCCTGCGGCCCCCGACCCTC CGGGCACTGGCGCTTCGGGGGCCACGGTCGTCTCAGGTTCAGCCTCAGGTCCTGCGGCTCCGGGTAAAGT TGGAAGTGTTGCTGTGGAAGACCAGCCAGATGTCAGTGCCGTGTTGTCAGCCTACAACCAACAAGGAGAT CCCACAATGTATGAAGAATACTATAGTGGACTGAAACACTTCATTGAATGTTCCCTGGACTGCCATCGGG CAGAGTTGTCCCAACTTTTTTATCCTCTGTTTGTGCACATGTACTTGGAGCTAGTCTACAATCAACATGA GAATGAAGCAAAGTCATTCTTTGAGAAGTTCCATGGAGATCAGGAATGTTATTACCAGGATGACCTACGA GTATTATCTAGTCTTACCAAAAAGGAACACATGAAAGGGAATGAGACCATGTTGGATTTTCGAACAAGTA AATTTGTTCTGCGTATTTCCCGTGACTCGTACCAACTCTTGAAGAGGCATCTTCAGGAGAAACAGAACAA TCAGATATGGAACATAGTTCAGGAGCACCTCTACATTGACATCTTTGATGGGATGCCGCGTAGTAAGCAA CAGATAGATGCGATGGTGGGAAGTTTGGCAGGAGAGGCTAAACGAGAGGCAAACAAATCAAAGGTATTTT TTGGTTTATTAAAAGAACCAGAAATTGAGGTACCTTTGGATGACGAGGATGAAGAGGGAGAAAATGAAGA AGGAAAACCTAAAAAGAAGAAGCCTAAAAAAGATAGTATTGGATCCAAAAGCAAAAAACAAGATCCCAAT GCTCCACCTCAGAACAGAATCCCTCTTCCTGAGTTGAAAGATTCAGATAAGTTGGATAAGATAATGAATA TGAAAGAAACCACCAAACGAGTGCGCCTTGGGCCGGACTGCTTACCCTCCATTTGTTTCTATACATTTCT CAATGCTTACCAGGGTCTCACTGCAGTGGATGTCACTGATGATTCTAGTCTGATTGCTGGAGGTTTTGCA GATTCAACTGTCAGAGTGTGGTCGGTAACACCCAAAAAGCTTCGTAGTGTCAAACAAGCATCAGATCTTA GTCTTATAGACAAAGAATCAGATGATGTCTTAGAAAGAATCATGGATGAGAAAACAGCAAGTGAGTTGAA GATTTTGTATGGTCACAGTGGGCCTGTCTACGGAGCCAGCTTCAGTCCGGATAGGAACTATCTGCTTTCC TCTTCAGAGGACGGAACTGTTAGATTGTGGAGCCTTCAAACATTTACTTGTTTGGTGGGATATAAAGGAC ACAACTATCCAGTATGGGACACACAATTTTCTCCATATGGATATTATTTTGTGTCAGGGGGCCATGACCG AGTAGCTCGGCTCTGGGCTACAGACCACTATCAGCCTTTAAGAATATTTGCCGGCCATCTTGCTGATGTG AATTGTACCAGATTCCATCCAAATTCTAATTATGTTGCTACGGGCTCTGCAGACAGAACTGTGCGGCTCT GGGACGTCCTGAATGGTAACTGTGTAAGGATCTTCACTGGACACAAGGGACCAATTCATTCCTTGACATT TTCTCCCAATGGGAGATTCCTGGCTACAGGAGCAACAGATGGCAGAGTGCTTCTTTGGGATATTGGACAT GGTTTGATGGTTGGAGAATTAAAAGGCCACACTGATACAGTCTGTTCACTTAGGTTTAGTAGAGATGGTG AAATTTTGGCATCAGGTTCAATGGATAATACAGTTCGATTATGGGATGCTATCAAAGCCTTTGAAGATTT AGAGACCGATGACTTTACTACAGCCACTGGGCATATAAATTTACCTGAGAATTCACAGGAGTTATTGTTG GGAACATATATGACCAAATCAACACCAGTTGTACACCTTCATTTTACTCTAAGAAACCTGGTTCTAGCTG CAGGAGCTTATAGTCCACAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208552 representing NM_006951
Red=Cloning site Green=Tags(s) MAALAEEQTEVAVKLEPEGPPTLLPPQAGDGAGEGSGGTTNNGPNGGGGNVAASSSTGGDGGTPKPTVAV SAAAPAGAAPVPAAAPDAGAPHDRQTLLAVLQFLRQSKLREAEEALRREAGLLEEAVAGSGAPGEVDSAG AEVTSALLSRVTASAPGPAAPDPPGTGASGATVVSGSASGPAAPGKVGSVAVEDQPDVSAVLSAYNQQGD PTMYEEYYSGLKHFIECSLDCHRAELSQLFYPLFVHMYLELVYNQHENEAKSFFEKFHGDQECYYQDDLR VLSSLTKKEHMKGNETMLDFRTSKFVLRISRDSYQLLKRHLQEKQNNQIWNIVQEHLYIDIFDGMPRSKQ QIDAMVGSLAGEAKREANKSKVFFGLLKEPEIEVPLDDEDEEGENEEGKPKKKKPKKDSIGSKSKKQDPN APPQNRIPLPELKDSDKLDKIMNMKETTKRVRLGPDCLPSICFYTFLNAYQGLTAVDVTDDSSLIAGGFA DSTVRVWSVTPKKLRSVKQASDLSLIDKESDDVLERIMDEKTASELKILYGHSGPVYGASFSPDRNYLLS SSEDGTVRLWSLQTFTCLVGYKGHNYPVWDTQFSPYGYYFVSGGHDRVARLWATDHYQPLRIFAGHLADV NCTRFHPNSNYVATGSADRTVRLWDVLNGNCVRIFTGHKGPIHSLTFSPNGRFLATGATDGRVLLWDIGH GLMVGELKGHTDTVCSLRFSRDGEILASGSMDNTVRLWDAIKAFEDLETDDFTTATGHINLPENSQELLL GTYMTKSTPVVHLHFTLRNLVLAAGAYSPQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006951 |
| ORF Size | 2400 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006951.3, NP_008882.2 |
| RefSeq Size | 3283 bp |
| RefSeq ORF | 2403 bp |
| Locus ID | 6877 |
| UniProt ID | Q15542 |
| Protein Families | Transcription Factors |
| Protein Pathways | Basal transcription factors |
| Gene Summary | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208552 | TAF5 (Myc-DDK-tagged)-Human TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa (TAF5) |
CNY 8,784.00 |
|
| RC208552L3 | Lenti-ORF clone of TAF5 (Myc-DDK-tagged)-Human TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa (TAF5) |
CNY 5,890.00 |
|
| RC208552L4 | Lenti-ORF clone of TAF5 (mGFP-tagged)-Human TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa (TAF5) |
CNY 5,890.00 |
|
| SC303858 | TAF5 (untagged)-Human TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa (TAF5) |
CNY 8,800.00 |
