Cathepsin O (CTSO) (NM_001334) Human Tagged ORF Clone

CAT#: RG208268

  • TrueORF®

CTSO (tGFP-tagged) - Human cathepsin O (CTSO)

ORF Plasmid: DDK tGFP



  "NM_001334" in other vectors (6)

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CNY 4,000.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms CTSO1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG208268 representing NM_001334
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGTGCGGGCGCTGCCGTGGCTGCCGTGGCTGCTGTGGCTGCTGTGCCGGGGCGGCGGCGATGCGG
ACTCCCGCGCCCCCTTCACCCCGACCTGGCCGCGGAGCCGCGAGCGTGAAGCCGCCGCCTTCCGGGAAAG
TCTTAATAGACATCGATACTTGAATTCTTTATTTCCCAGTGAAAACTCCACCGCCTTCTATGGAATAAAT
CAGTTTTCCTATTTGTTTCCTGAAGAGTTTAAAGCCATTTATTTAAGAAGCAAACCTTCCAAGTTTCCCA
GATACTCAGCAGAAGTACATATGTCCATCCCCAATGTGTCTTTGCCGTTAAGATTTGACTGGAGGGACAA
GCAGGTTGTGACACAAGTGAGAAACCAGCAGATGTGTGGAGGATGCTGGGCCTTCAGCGTGGTGGGGGCA
GTGGAATCTGCTTATGCAATAAAGGGGAAGCCCCTGGAAGACCTAAGTGTCCAGCAGGTCATTGACTGTT
CGTATAATAATTATGGCTGCAATGGAGGCTCTACTCTCAATGCTTTGAACTGGTTAAACAAGATGCAAGT
AAAACTGGTGAAAGATTCAGAATATCCTTTTAAAGCACAAAATGGTCTGTGCCATTACTTTTCTGGTTCA
CATTCTGGATTTTCAATCAAAGGTTATTCTGCATATGACTTCAGTGACCAAGAAGATGAAATGGCAAAAG
CACTTCTTACCTTTGGCCCTTTGGTAGTCATAGTAGATGCAGTGAGCTGGCAAGATTATCTGGGAGGCAT
TATACAGCATCACTGCTCTAGTGGAGAAGCAAATCATGCAGTTCTCATAACTGGGTTTGATAAAACAGGA
AGCACTCCATATTGGATTGTGCGGAATTCCTGGGGAAGTTCTTGGGGAGTAGATGGTTATGCCCATGTCA
AAATGGGAAGTAATGTTTGTGGTATTGCAGATTCCGTTTCTTCTATATTTGTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG208268 representing NM_001334
Red=Cloning site Green=Tags(s)

MDVRALPWLPWLLWLLCRGGGDADSRAPFTPTWPRSREREAAAFRESLNRHRYLNSLFPSENSTAFYGIN
QFSYLFPEEFKAIYLRSKPSKFPRYSAEVHMSIPNVSLPLRFDWRDKQVVTQVRNQQMCGGCWAFSVVGA
VESAYAIKGKPLEDLSVQQVIDCSYNNYGCNGGSTLNALNWLNKMQVKLVKDSEYPFKAQNGLCHYFSGS
HSGFSIKGYSAYDFSDQEDEMAKALLTFGPLVVIVDAVSWQDYLGGIIQHHCSSGEANHAVLITGFDKTG
STPYWIVRNSWGSSWGVDGYAHVKMGSNVCGIADSVSSIFV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001334
ORF Size 963 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001334.3
RefSeq Size 2943 bp
RefSeq ORF 966 bp
Locus ID 1519
UniProt ID P43234
Domains Pept_C1
Protein Families Druggable Genome, Protease
Protein Pathways Lysosome
Gene Summary The protein encoded by the gene is a cysteine proteinase and a member of the papain superfamily. This proteolytic enzyme is involved in cellular protein degradation and turnover. The recombinant form of this enzyme was shown to degrade synthetic peptides typically used as substrates for cysteine proteinases and its proteolytic activity was abolished by an inhibitor of cyteine proteinase. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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