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GRF2 (RAPGEF1) (NM_198679) Human Tagged ORF Clone
CAT＃: RG208092
- TrueORF®
RAPGEF1 (tGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2
ORF Plasmid: DDK
"NM_198679" in other vectors (4)
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CNY 9,224.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C3G; GRF2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208092 representing NM_198679
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAATGCTATTGAAAAACAGAAGCCCTTGAAACGAAGTCATCTGTACCCCTGGAAACAAGACTCTC AGCGTTCTCATCTCTCTTCCTTCACCATGAAGCTGATGGACAAATTCCACTCACCCAAAATCAAGAGAAC GCCATCAAAGAAGGGAAAACCAGCTGAGGTGTCCGTAAAGATTCCAGAGAAGCCTGTGAACAAAGAGGCA ACAGACAGATTTCTACCAGAGGGCTACCCTCTCCCCTTGGATCTGGAGCAGCAGGCAGTAGAATTTATGT CCACCAGTGCTGTGGCTTCCAGGTCTCAAAGGCAGAAGAACCTGAGCTGGCTGGAGGAGAAAGAGAAGGA AGTTGTCAGTGCCCTGCGCTACTTTAAGACCATTGTGGACAAAATGGCAATTGATAAGAAGGTACTGGAG ATGCTTCCAGGGTCAGCCAGCAAGGTGCTGGAGGCCATCTTACCCCTGGTGCAGAACGATCCTCGAATTC AGCACAGCTCAGCCCTCTCTTCCTGCTATAGCCGAGTGTACCAAAGCCTCGCCAACCTCATTCGCTGGTC TGACCAAGTGATGCTGGAAGGCGTGAACTCAGAAGACAAGGAGATGGTGACGACTGTGAAGGGGGTCATC AAGGCTGTGCTGGATGGAGTGAAGGAGCTGGTCAGGCTCACCATCGAGAAGCAGGGACGTCCGTCTCCGA CGAGCCCCGTGAAGCCCAGTTCCCCTGCCAGCAAGCCTGATGGCCCAGCAGAGCTCCCCCTGACAGACCG CGAGGTAGAGATCCTAAACAAGACGACTGGGATGTCACAGTCAACTGAGCTCCTCCCAGATGCCACGGAT GAAGAGGTCGCGCCCCCCAAGCCTCCTCTGCCTGGCATTCGGGTGGTTGATAATAGTCCTCCACCAGCAT TGCCACCCAAGAAAAGACAGTCGGCGCCGTCCCCTACCCGAGTGGCTGTGGTGGCCCCCATGAGCCGAGC CACCAGTGGCTCCAGTTTGCCTGTTGGAATCAATAGGCAGGATTTTGATGTTGACTGTTACGCACAGAGG CGACTGTCAGGAGGCAGCCACTCATATGGTGGAGAGTCGCCCCGCCTCTCCCCCTGCAGCAGCATAGGCA AGCTCAGCAAGTCAGACGAGCAGCTGTCCTCTCTGGACAGGGACAGTGGGCAGTGCTCCCGGAACACAAG CTGTGAAACACTAGACCACTATGATCCCGACTATGAATTCCTCCAGCAAGACCTCTCTAACGCAGACCAG ATACCTCAGCAGACGGCCTGGAACCTTAGCCCGTTGCCAGAGTCTTTGGGGGAGTCTGGGTCTCCATTTC TTGGCCCTCCTTTCCAGCTGCCTCTTGGCGGCCATCCCCAGCCAGACGGACCTCTGGCCCCAGGGCAGCA GACAGATACGCCACCTGCTCTCCCCGAGAAGAAGCGCAGGAGCGCAGCCTCCCAGACGGCGGACGGCTCT GGCTGCAGGGTGTCCTACGAGCGGCATCCCTCGCAGTATGACAACATCTCTGGGGAGGACCTGCAGAGCA CAGCCCCGATCCCATCCGTCCCCTACGCGCCCTTTGCTGCTATTCTGCCCTTTCAGCATGGAGGTTCCTC AGCCCCTGTCGAATTTGTGGGTGATTTTACTGCTCCTGAGTCAACCGGTGACCCAGAAAAACCACCTCCT CTACCAGAGAAGAAAAACAAACACATGCTGGCCTACATGCAGTTGCTGGAGGACTACTCGGAGCCGCAGC CCTCTATGTTCTACCAGACGCCACAGAACGAGCACATCTACCAGCAGAAGAACAAGCTCCTCATGGAGGT ATACGGCTTCAGCGACTCCTTCAGTGGGGTGGACTCCGTGCAGGAGCTGGCCCCGCCGCCCGCCCTACCC CCCAAGCAGCGGCAGCTGGAGCCACCGGCTGGGAAAGACGGACATCCCAGAGATCCCTCAGCGGTCAGCG GCGTCCCTGGGAAGGACAGCAGAGACGGCAGTGAGAGGGCCCCAAAGTCACCAGATGCTCTGGAGTCGGC TCAGTCGGAGGAGGAAGTGGACGAGCTGTCCCTCATTGACCACAACGAAATTATGTCCAGGCTGACGCTC AAGCAGGAGGGTGATGACGGGCCGGACGTCCGCGGAGGATCTGGGGACATCTTACTGGTCCATGCTACTG AGACTGACAGGAAAGATTTGGTGTTGTACTGCGAGGCATTCCTGACCACCTACAGGACCTTCATCTCCCC AGAGGAGCTCATCAAGAAGCTGCAGTACAGATATGAGAAATTCTCTCCCTTTGCCGACACATTCAAGAAG CGCGTCAGCAAGAACACGTTCTTCGTGCTGGTACGGGTGGTGGATGAGCTCTGCCTGGTGGAGTTGACAG AAGAGATCCTGAAGCTGCTGATGGAACTGGTCTTCCGCCTGGTGTGCAATGGGGAGCTGAGCCTGGCCCG TGTGCTCCGGAAGAACATCCTGGACAAGGTGGACCAGAAGAAGCTACTCAGGTGTGCCACCTCCAGCCAG CCCCTGGCAGCCCGGGGGGTAGCAGCCAGGCCGGGGACCTTGCACGACTTTCACAGCCATGAGATAGCGG AGCAGCTAACGCTGCTGGATGCTGAGCTCTTCTATAAAATAGAGATTCCTGAGGTTTTGCTTTGGGCAAA AGAGCAGAATGAGGAGAAGAGCCCCAACTTGACCCAGTTCACGGAGCACTTCAACAACATGTCCTACTGG GTCCGGTCCATAATCATGTTACAGGAAAAGGCCCAGGACAGGGAACGGCTGCTCTTGAAGTTCATCAAGA TCATGAAGCACTTGCGGAAGCTGAATAACTTCAACTCCTACTTGGCCATCCTCTCTGCCCTGGACTCGGC GCCCATCCGCAGGCTGGAGTGGCAGAAGCAGACTTCAGAGGGCCTGGCCGAGTACTGCACACTGATCGAC AGCTCGTCCTCCTTCCGAGCCTACCGGGCCGCCCTCTCGGAGGTGGAACCGCCGTGCATCCCGTACCTGG GGCTGATCCTGCAGGACCTGACCTTCGTTCACCTGGGAAACCCAGACTACATCGACGGGAAAGTGAACTT CTCCAAGCGGTGGCAGCAGTTCAACATCCTCGACAGCATGCGCTGCTTCCAGCAGGCGCACTATGACATG CGGAGGAACGACGACATTATAAACTTCTTCAATGACTTCAGTGACCACCTGGCTGAGGAGGCCCTATGGG AACTGTCTCTGAAAATTAAACCCAGGAACATAACAAGGAGAAAAACAGACCGGGAAGAGAAGACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208092 representing NM_198679
Red=Cloning site Green=Tags(s) MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEA TDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLE MLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVI KAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATD EEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQR RLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQ IPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGS GCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPP LPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALP PKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTL KQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKK RVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQ PLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYW VRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLID SSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDM RRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198679 |
| ORF Size | 3285 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198679.2 |
| RefSeq Size | 6204 bp |
| RefSeq ORF | 3288 bp |
| Locus ID | 2889 |
| UniProt ID | Q13905 |
| Protein Families | Druggable Genome |
| Protein Pathways | Focal adhesion, Insulin signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma |
| Gene Summary | This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208092 | RAPGEF1 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2 |
CNY 7,624.00 |
|
| RC208092L3 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2, Myc-DDK-tagged |
CNY 12,830.00 |
|
| RC208092L4 | Lenti ORF clone of Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2, mGFP tagged |
CNY 12,830.00 |
|
| SC307763 | RAPGEF1 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2 |
CNY 7,632.00 |
