KCNJ14 (NM_013348) Human Tagged ORF Clone

CAT#: RG207756

  • TrueORF®

KCNJ14 (tGFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 14 (KCNJ14), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_013348" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

CNY 4,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms IRK4; KIR2.4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG207756 representing NM_013348
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCTGGCCAGGGCCCTACGCCGCCTCAGCGGCGCCCTGGATTCGGGAGACAGCCGGGCGGGCGATG
AAGAGGAGGCCGGGCCCGGGTTGTGCCGCAACGGGTGGGCGCCGGCACCGGTGCAGTCACCCGTGGGCCG
GCGCCGCGGTCGCTTCGTCAAGAAAGACGGGCACTGCAACGTGCGTTTCGTAAACCTGGGTGGCCAGGGC
GCGCGCTACCTGAGCGACCTGTTCACCACATGCGTGGACGTGCGCTGGCGCTGGATGTGCCTGCTCTTCT
CCTGCTCCTTCCTCGCCTCCTGGCTGCTCTTCGGCCTGGCCTTCTGGCTCATTGCCTCGCTGCACGGCGA
CCTGGCCGCCCCGCCACCGCCCGCGCCCTGCTTCTCACACGTGGCCAGCTTCCTGGCCGCCTTCCTCTTC
GCGCTGGAGACGCAGACGTCCATCGGCTACGGCGTGCGCAGCGTCACCGAGGAGTGCCCGGCCGCTGTGG
CCGCCGTGGTGCTGCAGTGCATTGCCGGCTGCGTGCTCGACGCCTTCGTCGTGGGTGCTGTCATGGCCAA
GATGGCCAAACCCAAGAAGCGCAACGAGACGCTGGTCTTCAGCGAGAACGCCGTCGTGGCGCTGCGCGAC
CACCGCCTCTGCCTCATGTGGCGCGTCGGCAACCTGCGCCGCAGCCACCTGGTCGAGGCCCACGTGCGTG
CCCAGCTGCTGCAGCCCCGTGTGACCCCAGAGGGTGAGTACATCCCGCTGGACCACCAGGATGTGGATGT
GGGCTTTGATGGAGGCACCGATCGTATCTTCCTCGTGTCCCCCATCACCATCGTCCATGAGATCGACTCT
GCCAGTCCTCTGTATGAGCTAGGACGTGCCGAGCTGGCCAGGGCTGACTTTGAGCTGGTGGTCATTCTCG
AGGGGATGGTTGAGGCCACAGCCATGACCACACAGTGTCGCTCGTCCTACCTCCCTGGTGAACTGCTCTG
GGGCCATCGTTTTGAGCCAGTTCTCTTCCAGCGTGGCTCCCAGTATGAGGTCGACTATCGCCACTTCCAT
CGCACTTATGAGGTCCCAGGGACACCGGTCTGCAGTGCTAAGGAGCTGGATGAACGGGCAGAGCAGGCTT
CCCACAGCCTCAAGTCTAGTTTCCCCGGCTCTCTGACTGCATTTTGTTATGAGAATGAACTTGCTCTGAG
CTGCTGCCAGGAGGAAGATGAGGACGATGAGACTGAGGAAGGGAATGGGGTGGAAACAGAAGATGGGGCT
GCTAGCCCCCGAGTTCTCACACCAACCCTGGCGCTGACCCTGCCTCCA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG207756 representing NM_013348
Red=Cloning site Green=Tags(s)

MGLARALRRLSGALDSGDSRAGDEEEAGPGLCRNGWAPAPVQSPVGRRRGRFVKKDGHCNVRFVNLGGQG
ARYLSDLFTTCVDVRWRWMCLLFSCSFLASWLLFGLAFWLIASLHGDLAAPPPPAPCFSHVASFLAAFLF
ALETQTSIGYGVRSVTEECPAAVAAVVLQCIAGCVLDAFVVGAVMAKMAKPKKRNETLVFSENAVVALRD
HRLCLMWRVGNLRRSHLVEAHVRAQLLQPRVTPEGEYIPLDHQDVDVGFDGGTDRIFLVSPITIVHEIDS
ASPLYELGRAELARADFELVVILEGMVEATAMTTQCRSSYLPGELLWGHRFEPVLFQRGSQYEVDYRHFH
RTYEVPGTPVCSAKELDERAEQASHSLKSSFPGSLTAFCYENELALSCCQEEDEDDETEEGNGVETEDGA
ASPRVLTPTLALTLPP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_013348
ORF Size 1308 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_013348.4
RefSeq Size 3049 bp
RefSeq ORF 1311 bp
Locus ID 3770
UniProt ID Q9UNX9
Protein Families Druggable Genome, Ion Channels: Potassium, Transmembrane
Gene Summary Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel, and probably has a role in controlling the excitability of motor neurons. [provided by RefSeq, Feb 2013]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...