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PNK (PNKP) (NM_007254) Human Tagged ORF Clone
CAT＃: RG207551
- TrueORF®
PNKP (tGFP-tagged) - Human polynucleotide kinase 3'-phosphatase (PNKP)
ORF Plasmid: DDK
"NM_007254" in other vectors (7)
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CNY 5,040.00
| Cited in 2 publications. |
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AOA4; CMT2B2; EIEE10; MCSZ; PNK |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG207551 representing NM_007254
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGAGGTGGAGGCCCCGGGCCGCTTGTGGCTCGAGAGCCCCCCTGGGGGAGCGCCCCCCATCTTCC TGCCCTCGGACGGGCAAGCCCTGGTCCTGGGCAGGGGACCCCTGACCCAGGTTACGGACCGGAAGTGCTC CAGAACTCAAGTGGAGCTGGTCGCAGATCCTGAGACCCGGACAGTGGCAGTGAAACAGCTGGGAGTTAAC CCCTCAACTACCGGGACCCAGGAGTTGAAGCCGGGGTTGGAGGGCTCTCTGGGGGTGGGGGACACACTGT ATTTGGTCAATGGCCTCCACCCACTGACCCTGCGCTGGGAAGAGACCCGCACACCAGAATCCCAGCCAGA TACTCCGCCTGGCACCCCTCTGGTGTCCCAAGATGAGAAGAGAGATGCTGAGCTGCCGAAGAAGCGTATG CGGAAGTCAAACCCCGGCTGGGAGAACTTGGAGAAGTTGCTAGTGTTCACCGCAGCTGGGGTGAAACCCC AGGGCAAGGTGGCTGGCTTTGATCTGGACGGGACGCTCATCACCACACGCTCTGGGAAGGTCTTTCCCAC TGGCCCCAGTGACTGGAGGATCTTGTACCCAGAGATTCCCCGTAAGCTCCGAGAGCTGGAAGCCGAGGGC TACAAGCTGGTGATCTTCACCAACCAGATGAGCATCGGGCGCGGGAAGCTGCCAGCCGAGGAGTTCAAGG CCAAGGTGGAGGCTGTGGTGGAGAAGCTGGGGGTCCCCTTCCAGGTGCTGGTGGCCACGCACGCAGGCTT GTACCGGAAGCCGGTGACGGGCATGTGGGACCATCTGCAGGAGCAGGCCAACGACGGCACGCCCATATCC ATCGGGGACAGCATCTTTGTGGGAGACGCAGCCGGACGCCCGGCCAACTGGGCCCCGGGGCGGAAGAAGA AAGACTTCTCCTGCGCCGATCGCCTGTTTGCCCTCAACCTTGGCCTGCCCTTCGCCACGCCTGAGGAGTT CTTTCTCAAGTGGCCAGCAGCCGGCTTCGAGCTCCCAGCCTTTGATCCGAGGACTGTCTCCCGCTCAGGG CCTCTCTGCCTCCCCGAGTCCAGGGCCCTCCTGAGCGCCAGCCCGGAGGTGGTTGTCGCAGTGGGATTCC CTGGGGCCGGGAAGTCCACCTTTCTCAAGAAGCACCTCGTGTCGGCCGGATATGTCCACGTGAACAGGGA CACGCTAGGCTCCTGGCAGCGCTGTGTGACCACGTGTGAGACAGCCCTGAAGCAAGGGAAACGGGTCGCC ATCGACAACACAAACCCAGACGCCGCGAGCCGCGCCAGGTACGTCCAGTGTGCCCGAGCCGCGGGCGTCC CCTGCCGCTGCTTCCTCTTCACCGCCACTCTGGAGCAGGCGCGCCACAACAACCGGTTTCGAGAGATGAC GGACTCCTCTCATATCCCCGTGTCAGACATGGTCATGTATGGCTACAGGAAGCAGTTCGAGGCCCCAACG CTGGCTGAAGGCTTCTCTGCCATCCTGGAGATCCCGTTCCGGCTATGGGTGGAGCCGAGGCTGGGGCGGC TGTACTGCCAGTTCTCCGAGGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207551 representing NM_007254
Red=Cloning site Green=Tags(s) MGEVEAPGRLWLESPPGGAPPIFLPSDGQALVLGRGPLTQVTDRKCSRTQVELVADPETRTVAVKQLGVN PSTTGTQELKPGLEGSLGVGDTLYLVNGLHPLTLRWEETRTPESQPDTPPGTPLVSQDEKRDAELPKKRM RKSNPGWENLEKLLVFTAAGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEG YKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPIS IGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSG PLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVA IDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPT LAEGFSAILEIPFRLWVEPRLGRLYCQFSEG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007254 |
| ORF Size | 1563 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007254.4 |
| RefSeq Size | 1745 bp |
| RefSeq ORF | 1566 bp |
| Locus ID | 11284 |
| UniProt ID | Q96T60 |
| Protein Families | Druggable Genome, Phosphatase |
| Gene Summary | This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
XRCC1 phosphorylation affects aprataxin recruitment and DNA deadenylation activity
,null,
DNA repair
,PubMed ID 29477978
[PNKP]
|
|
XRCC1-mediated repair of strand breaks independent of PNKP binding
,null,
DNA repair
,PubMed ID 29100039
[PNKP]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207551 | PNKP (Myc-DDK-tagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 3,880.00 |
|
| RC207551L1 | Lenti-ORF clone of PNKP (Myc-DDK-tagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 6,280.00 |
|
| RC207551L2 | Lenti-ORF clone of PNKP (mGFP-tagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 6,460.00 |
|
| RC207551L3 | Lenti-ORF clone of PNKP (Myc-DDK-tagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 6,280.00 |
|
| RC207551L4 | Lenti-ORF clone of PNKP (mGFP-tagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 6,460.00 |
|
| SC128040 | PNKP (untagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 2,950.00 |
|
| SC323976 | PNKP (untagged)-Human polynucleotide kinase 3'-phosphatase (PNKP) |
CNY 2,950.00 |
