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Glycerol kinase (GK) (NM_203391) Human Tagged ORF Clone
CAT＃: RG207264
- TrueORF®
GK (tGFP-tagged) - Human glycerol kinase (GK), transcript variant 1
ORF Plasmid: DDK
"NM_203391" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GK1; GKD |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG207264 representing NM_203391
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCTCAAAGAAGGCAGTTTTGGGGCCATTGGTGGGGGCGGTGGACCAGGGCACCAGTTCGACGC GCTTTTTGGTTTTCAATTCAAAAACAGCTGAACTACTTAGTCATCATCAAGTAGAAATAAAACAAGAGTT CCCAAGAGAAGGATGGGTGGAACAGGACCCTAAGGAAATTCTACATTCTGTCTATGAGTGTATAGAGAAA ACATGTGAGAAACTTGGACAGCTCAAAATTGATATTTCCAACATAAAAGCTATTGGTGTCAGCAACCAGA GGGAAACCACTGTAGTCTGGGACAAGATAACTGGAGAGCCTCTCTACAATGCTGTGGTGTGGCTTGATCT AAGAACCCAGTCTACCGTTGAGAGTCTTAGTAAAAGAATTACAGGAAATAATAACTTTGTCAAGTCCAAG ACAGGCCTTCCACTTAGCACTTACTTCAGTGCAGTGAAACTTCGTTGGCTCCTTGACAATGTGAGAAAAG TTCAAAAGGCAGTTGAAGAAAAACGAGCTCTTTTTGGGACTATTGATTCATGGCTTATTTGGAGTTTGAC AGGAGGAGTCAATGGAGGTGTCCACTGTACAGATGTAACAAATGCAAGTAGGACTATGCTTTTCAACATT CATTCTTTGGAATGGGATAAACAACTCTGCGAATTTTTTGGAATTCCAATGGAAATTCTTCCAAATGTCC GGAGTTCTTCTGAGATCTATGGCCTAATGAAAATCTCTCATAGCGTGAAAGCTGGGGCCTTGGAAGGTGT GCCAATATCTGGGTGTTTAGGGGACCAGTCTGCTGCATTGGTGGGACAAATGTGCTTCCAGATTGGACAA GCCAAAAATACGTATGGAACAGGATGTTTCTTACTATGTAATACAGGCCATAAGTGTGTATTTTCTGATC ATGGCCTTCTCACCACAGTGGCTTACAAACTTGGCAGAGACAAACCAGTATATTATGCTTTGGAAGGTTC TGTAGCTATAGCTGGTGCTGTTATTCGCTGGCTAAGAGACAATCTTGGAATTATAAAGACCTCAGAAGAA ATTGAAAAACTTGCTAAAGAAGTAGGTACTTCTTATGGCTGCTACTTCGTCCCAGCATTTTCGGGGTTAT ATGCACCTTATTGGGAGCCCAGCGCAAGAGGGATAATCTGTGGACTCACTCAGTTCACCAATAAATGCCA TATTGCTTTTGCTGCATTAGAAGCTGTTTGTTTCCAAACTCGAGAGATTTTGGATGCCATGAATCGAGAC TGTGGAATTCCACTCAGTCATTTGCAGGTAGATGGAGGAATGACCAGCAACAAAATTCTTATGCAGCTAC AAGCAGACATTCTGTATATACCAGTAGTGAAGCCCTCAATGCCCGAAACCACTGCACTGGGTGCGGCTAT GGCGGCAGGGGCTGCAGAAGGAGTCGGCGTATGGAGTCTCGAACCCGAGGATTTGTCTGCCGTCACGATG GAGCGGTTTGAACCTCAGATTAATGCGGAGGAAAGTGAAATTCGTTATTCTACATGGAAGAAAGCTGTGA TGAAGTCAATGGGTTGGGTTACAACTCAATCTCCAGAAAGTGGTATTCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207264 representing NM_203391
Red=Cloning site Green=Tags(s) MAASKKAVLGPLVGAVDQGTSSTRFLVFNSKTAELLSHHQVEIKQEFPREGWVEQDPKEILHSVYECIEK TCEKLGQLKIDISNIKAIGVSNQRETTVVWDKITGEPLYNAVVWLDLRTQSTVESLSKRITGNNNFVKSK TGLPLSTYFSAVKLRWLLDNVRKVQKAVEEKRALFGTIDSWLIWSLTGGVNGGVHCTDVTNASRTMLFNI HSLEWDKQLCEFFGIPMEILPNVRSSSEIYGLMKISHSVKAGALEGVPISGCLGDQSAALVGQMCFQIGQ AKNTYGTGCFLLCNTGHKCVFSDHGLLTTVAYKLGRDKPVYYALEGSVAIAGAVIRWLRDNLGIIKTSEE IEKLAKEVGTSYGCYFVPAFSGLYAPYWEPSARGIICGLTQFTNKCHIAFAALEAVCFQTREILDAMNRD CGIPLSHLQVDGGMTSNKILMQLQADILYIPVVKPSMPETTALGAAMAAGAAEGVGVWSLEPEDLSAVTM ERFEPQINAEESEIRYSTWKKAVMKSMGWVTTQSPESGIP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_203391 |
| ORF Size | 1590 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_203391.1, NP_976325.1 |
| RefSeq Size | 3591 bp |
| RefSeq ORF | 1593 bp |
| Locus ID | 2710 |
| UniProt ID | P32189 |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerolipid metabolism, Metabolic pathways, PPAR signaling pathway |
| Gene Summary | The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207264 | GK (Myc-DDK-tagged)-Human glycerol kinase (GK), transcript variant 1 |
CNY 3,944.00 |
|
| RC207264L3 | Lenti ORF clone of Human glycerol kinase (GK), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207264L4 | Lenti ORF clone of Human glycerol kinase (GK), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC308150 | GK (untagged)-Human glycerol kinase (GK), transcript variant 1 |
CNY 7,220.00 |
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