United States
Germany
Japan
United Kingdom
China
POLDIP1 (KCTD13) (NM_178863) Human Tagged ORF Clone
CAT＃: RG206944
- TrueORF®
KCTD13 (tGFP-tagged) - Human potassium channel tetramerisation domain containing 13 (KCTD13)
ORF Plasmid: DDK
"NM_178863" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 5,200.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BACURD1; FKSG86; hBACURD1; PDIP1; POLDIP1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG206944 representing NM_178863
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCGGAGGCCTCGGGCCCGGCTGCCGCCGCGGCCCCGTCCCTGGAAGCCCCCAAGCCCTCGGGTC TCGAGCCTGGCCCCGCCGCCTACGGTCTCAAGCCGCTGACCCCGAACAGCAAATACGTGAAGCTGAACGT GGGCGGCTCGTTGCACTACACCACGCTGCGCACCCTCACGGGACAGGACACCATGCTCAAAGCCATGTTC AGCGGCCGCGTGGAGGTGCTGACCGATGCCGGAGGTTGGGTGCTGATTGACCGGAGCGGCCGTCACTTTG GTACAATCCTCAATTACCTGCGGGATGGGTCTGTGCCACTGCCGGAGAGTACGAGAGAACTGGGGGAGCT GCTGGGCGAAGCACGCTACTACCTGGTGCAGGGCCTGATTGAGGACTGCCAGCTGGCGCTGCAGCAAAAA AGGGAGACGCTGTCCCCGCTGTGCCTCATCCCCATGGTGACATCTCCCCGGGAGGAGCAGCAGCTCCTGG CCAGCACCTCCAAGCCCGTGGTGAAGCTCCTGCACAACCGCAGTAACAACAAGTACTCCTACACCAGCAC TTCAGATGACAACCTACTTAAGAACATCGAGCTGTTCGACAAGCTGGCCCTGCGCTTCCACGGGCGGCTA CTCTTCCTCAAGGATGTCCTGGGGGACGAGATCTGTTGCTGGTCTTTCTACGGGCAGGGCCGCAAAATCG CCGAGGTGTGCTGCACCTCCATTGTCTATGCTACGGAGAAGAAGCAGACCAAGGTGGAATTTCCAGAGGC CCGGATCTTCGAGGAGACCCTGAACATCCTCATCTACGAGACTCCCCGGGGCCCAGACCCAGCCCTCCTG GAGGCCACAGGGGGAGCAGCTGGAGCTGGTGGGGCTGGCCGCGGGGAGGATGAAGAGAACCGAGAGCACC GTGTCCGCAGGATCCATGTCCGGCGCCATATCACCCACGACGAGCGTCCTCATGGCCAACAAATTGTCTT CAAGGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG206944 representing NM_178863
Red=Cloning site Green=Tags(s) MSAEASGPAAAAAPSLEAPKPSGLEPGPAAYGLKPLTPNSKYVKLNVGGSLHYTTLRTLTGQDTMLKAMF SGRVEVLTDAGGWVLIDRSGRHFGTILNYLRDGSVPLPESTRELGELLGEARYYLVQGLIEDCQLALQQK RETLSPLCLIPMVTSPREEQQLLASTSKPVVKLLHNRSNNKYSYTSTSDDNLLKNIELFDKLALRFHGRL LFLKDVLGDEICCWSFYGQGRKIAEVCCTSIVYATEKKQTKVEFPEARIFEETLNILIYETPRGPDPALL EATGGAAGAGGAGRGEDEENREHRVRRIHVRRHITHDERPHGQQIVFKD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178863 |
| ORF Size | 987 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178863.2, NP_849194.1 |
| RefSeq Size | 1750 bp |
| RefSeq ORF | 990 bp |
| Locus ID | 253980 |
| UniProt ID | Q8WZ19 |
| Protein Families | Ion Channels: Other, Transcription Factors |
| Gene Summary | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for synaptic transmission (PubMed:19782033). The BCR(KCTD13) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome (PubMed:19782033) Degradation of RHOA regulates the actin cytoskeleton and promotes synaptic transmission (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206944 | KCTD13 (Myc-DDK-tagged)-Human potassium channel tetramerisation domain containing 13 (KCTD13) |
CNY 3,600.00 |
|
| RC206944L1 | Lenti ORF clone of Human potassium channel tetramerisation domain containing 13 (KCTD13), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC206944L2 | Lenti ORF clone of Human potassium channel tetramerisation domain containing 13 (KCTD13), mGFP tagged |
CNY 5,890.00 |
|
| RC206944L3 | Lenti ORF clone of Human potassium channel tetramerisation domain containing 13 (KCTD13), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206944L4 | Lenti ORF clone of Human potassium channel tetramerisation domain containing 13 (KCTD13), mGFP tagged |
CNY 6,000.00 |
|
| SC107237 | KCTD13 (untagged)-Human potassium channel tetramerisation domain containing 13 (KCTD13) |
CNY 3,600.00 |
其它POLDIP1产品
