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PIWIL4 (NM_152431) Human Tagged ORF Clone
CAT＃: RG206117
- TrueORF®
PIWIL4 (tGFP-tagged) - Human piwi-like 4 (Drosophila) (PIWIL4)
ORF Plasmid: DDK
"NM_152431" in other vectors (6)
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CNY 7,840.00
CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HIWI2; MIWI2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG206117 representing NM_152431
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGAAGAGCCCGAGTGAAGGCCAGAGGCATCGCCCGCAGCCCCAGTGCCACAGAAGTGGGGCGCA TCCAAGCCTCGCCATTGCCTAGATCTGTTGATCTTAGTAACAATGAAGCATCCTCTAGCAATGGCTTCTT GGGAACAAGCAGGATCTCAACCAACGATAAATATGGGATATCTTCTGGTGATGCTGGAAGTACCTTCATG GAAAGAGGTGTGAAAAACAAACAGGACTTTATGGATTTGAGTATCTGTACCAGAGAAAAATTGGCACATG TGAGAAATTGTAAAACAGGTTCCAGTGGAATACCTGTGAAACTGGTTACAAACCTCTTTAACTTAGATTT TCCCCAAGACTGGCAGCTATACCAGTACCATGTGACATATATTCCAGATTTAGCATCTAGAAGGCTGAGA ATTGCTTTACTTTATAGTCATAGTGAACTTTCCAACAAAGCAAAAGCATTCGACGGTGCCATCCTTTTTC TGTCACAAAAGCTAGAAGAAAAGGTCACAGAGTTGTCAAGTGAAACTCAAAGAGGTGAGACTATAAAGAT GACTATCACCCTGAAGAGGGAGCTGCCATCAAGTTCTCCCGTGTGCATCCAGGTCTTCAATATCATCTTC AGAAAGATCCTCAAAAAGTTGTCCATGTACCAAATTGGACGGAACTTCTATAATCCTTCAGAGCCAATGG AAATTCCCCAGCACAAATTATCCCTTTGGCCTGGGTTTGCCATTTCTGTGTCATATTTTGAAAGGAAGCT CCTGTTTAGTGCTGATGTGAGTTACAAAGTCCTCCGGAATGAGACGGTTCTGGAATTCATGACTGCTCTC TGTCAAAGAACTGGCTTGTCCTGTTTCACCCAGACGTGTGAGAAGCAGCTAATAGGGCTCATTGTCCTTA CAAGATACAATAACAGAACCTACTCCATTGATGACATTGACTGGTCAGTGAAGCCCACACACACCTTTCA GAAGCGGGATGGCACCGAGATCACCTATGTGGATTACTACAAGCAGCAGTATGATATTACTGTATCGGAC CTGAATCAGCCCATGCTTGTTAGTCTGTTAAAGAAGAAGAGAAATGACAACAGTGAGGCTCAGCTCGCCC ACCTGATACCTGAGCTCTGCTTTCTAACAGGGCTGACTGACCAGGCAACATCTGATTTCCAGCTGATGAA GGCTGTGGCTGAAAAGACACGTCTCAGTCCTTCAGGCCGGCAGCAGCGCCTGGCCAGGCTTGTGGACAAC ATCCAGAGGAATACCAATGCTCGCTTTGAACTAGAGACCTGGGGACTGCATTTTGGAAGCCAGATATCTC TGACTGGCCGGATTGTGCCTTCAGAAAAAATATTAATGCAAGACCACATATGTCAACCTGTGTCTGCTGC TGACTGGTCCAAGGATATTCGAACTTGCAAGATTTTAAATGCACAGTCTTTGAATACCTGGTTGATTTTA TGTAGCGACAGAACTGAATATGTTGCCGAGAGCTTTCTGAACTGCTTGAGAAGAGTTGCAGGTTCCATGG GATTTAATGTGGACTACCCCAAAATCATAAAAGTACAAGAAAATCCAGCTGCATTTGTTAGAGCTATACA GCAATATGTTGATCCTGATGTTCAGCTGGTAATGTGCATTCTGCCTTCTAATCAGAAGACCTATTATGAT TCCATTAAAAAATATTTGAGCTCAGACTGCCCAGTCCCAAGCCAATGTGTGCTTGCTCGGACCTTGAATA AACAGGGCATGATGATGAGTATCGCCACCAAGATCGCTATGCAGATGACTTGCAAGCTCGGAGGCGAGCT GTGGGCTGTGGAAATACCTTTAAAGTCCCTGATGGTGGTCGGTATTGATGTCTGTAAAGATGCACTCAGC AAGGACGTGATGGTTGTTGGATGCGTGGCAAGTGTTAACCCCAGAATCACCAGGTGGTTTTCCCGCTGTA TCCTTCAGAGAACAATGACTGATGTTGCAGATTGCTTGAAAGTTTTCATGACTGGAGCACTCAACAAATG GTACAAGTACAATCATGATTTGCCAGCACGGATAATTGTGTACCGTGCTGGTGTAGGGGATGGTCAGCTG AAAACACTTATTGAATATGAAGTCCCACAGCTGCTGAGCAGTGTGGCAGAATCCAGCTCAAATACCAGCT CAAGACTGTCGGTGATTGTGGTCAGGAAGAAGTGCATGCCACGATTCTTTACCGAAATGAACCGCACTGT ACAGAACCCCCCACTTGGCACTGTTGTGGATTCAGAAGCAACACGTAACGAATGGTATGACTTTTATCTG ATCAGCCAGGTGGCCTGCCGGGGAACTGTTAGTCCTACCTACTATAATGTCATCTATGATGACAACGGCT TGAAGCCCGACCATATGCAGAGACTTACATTCAAATTGTGCCACCTGTACTACAACTGGCCGGGCATAGT CAGTGTCCCAGCACCATGTCAGTATGCTCACAAGCTGACCTTTCTGGTGGCACAAAGCATTCATAAAGAA CCCAGTCTGGAATTAGCCAACCATCTCTTCTACCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG206117 representing NM_152431
Red=Cloning site Green=Tags(s) MSGRARVKARGIARSPSATEVGRIQASPLPRSVDLSNNEASSSNGFLGTSRISTNDKYGISSGDAGSTFM ERGVKNKQDFMDLSICTREKLAHVRNCKTGSSGIPVKLVTNLFNLDFPQDWQLYQYHVTYIPDLASRRLR IALLYSHSELSNKAKAFDGAILFLSQKLEEKVTELSSETQRGETIKMTITLKRELPSSSPVCIQVFNIIF RKILKKLSMYQIGRNFYNPSEPMEIPQHKLSLWPGFAISVSYFERKLLFSADVSYKVLRNETVLEFMTAL CQRTGLSCFTQTCEKQLIGLIVLTRYNNRTYSIDDIDWSVKPTHTFQKRDGTEITYVDYYKQQYDITVSD LNQPMLVSLLKKKRNDNSEAQLAHLIPELCFLTGLTDQATSDFQLMKAVAEKTRLSPSGRQQRLARLVDN IQRNTNARFELETWGLHFGSQISLTGRIVPSEKILMQDHICQPVSAADWSKDIRTCKILNAQSLNTWLIL CSDRTEYVAESFLNCLRRVAGSMGFNVDYPKIIKVQENPAAFVRAIQQYVDPDVQLVMCILPSNQKTYYD SIKKYLSSDCPVPSQCVLARTLNKQGMMMSIATKIAMQMTCKLGGELWAVEIPLKSLMVVGIDVCKDALS KDVMVVGCVASVNPRITRWFSRCILQRTMTDVADCLKVFMTGALNKWYKYNHDLPARIIVYRAGVGDGQL KTLIEYEVPQLLSSVAESSSNTSSRLSVIVVRKKCMPRFFTEMNRTVQNPPLGTVVDSEATRNEWYDFYL ISQVACRGTVSPTYYNVIYDDNGLKPDHMQRLTFKLCHLYYNWPGIVSVPAPCQYAHKLTFLVAQSIHKE PSLELANHLFYL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152431 |
| ORF Size | 2556 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_152431.1, NP_689644.1 |
| RefSeq Size | 3138 bp |
| RefSeq ORF | 2559 bp |
| Locus ID | 143689 |
| UniProt ID | Q7Z3Z4 |
| Protein Pathways | Dorso-ventral axis formation |
| Gene Summary | PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206117 | PIWIL4 (Myc-DDK-tagged)-Human piwi-like 4 (Drosophila) (PIWIL4) |
CNY 6,240.00 |
|
| RC206117L1 | Lenti ORF clone of Human piwi-like 4 (Drosophila) (PIWIL4), Myc-DDK-tagged |
CNY 8,640.00 |
|
| RC206117L2 | Lenti ORF clone of Human piwi-like 4 (Drosophila) (PIWIL4), mGFP tagged |
CNY 9,030.00 |
|
| RC206117L3 | Lenti ORF clone of Human piwi-like 4 (Drosophila) (PIWIL4), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC206117L4 | Lenti ORF clone of Human piwi-like 4 (Drosophila) (PIWIL4), mGFP tagged |
CNY 9,030.00 |
|
| SC306397 | PIWIL4 (untagged)-Human piwi-like 4 (Drosophila) (PIWIL4) |
CNY 6,248.00 |
