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PIGM (NM_145167) Human Tagged ORF Clone
CAT＃: RG205766
- TrueORF®
PIGM (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class M (PIGM)
ORF Plasmid: DDK
"NM_145167" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GPI-MT-I |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205766 representing NM_145167
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCCACCAAGCACTGGGGCGAATGGCTCCTGAACTTGAAGGTGGCTCCAGCCGGCGTCTTTGGTG TGGCCTTTCTAGCCAGAGTCGCCCTGGTTTTCTATGGCGTCTTCCAGGACCGGACCCTGCACGTGAGGTA TACGGACATCGACTACCAGGTCTTCACCGACGCCGCGCGCTTCGTCACGGAGGGGCGCTCGCCTTACCTG AGAGCCACGTACCGTTACACCCCGCTGCTGGGTTGGCTCCTCACTCCCAACATCTACCTCAGCGAGCTCT TTGGAAAGTTTCTCTTCATCAGCTGCGACCTCCTCACCGCTTTCCTCTTATACCGCCTGCTGCTGCTGAA GGGGCTGGGGCGCCGCCAGGCTTGTGGCTACTGTGTCTTTTGGCTTCTTAACCCCCTGCCTATGGCAGTA TCCAGCCGCGGTAATGCGGACTCTATTGTCGCCTCCCTGGTCCTGATGGTCCTCTACTTGATAAAGAAAA GACTCGTCGCGTGTGCAGCTGTATTCTATGGTTTCGCGGTGCATATGAAGATATATCCAGTGACTTACAT CCTTCCCATAACCCTCCACCTGCTTCCAGATCGCGACAATGACAAAAGCCTCCGTCAATTCCGGTACACT TTCCAGGCTTGTTTGTACGAGCTCCTGAAAAGGCTGTGTAATCGGGCTGTGCTGCTGTTTGTAGCAGTTG CTGGACTCACGTTTTTTGCCCTGAGCTTTGGTTTTTACTATGAGTACGGCTGGGAATTTTTGGAACACAC CTACTTTTATCACCTGACTAGGCGGGATATCCGTCACAACTTTTCTCCGTACTTCTACATGCTGTATTTG ACTGCAGAGAGCAAGTGGAGTTTTTCCCTGGGAATTGCTGCATTCCTGCCACAGCTCATCTTGCTTTCAG CTGTGTCTTTCGCCTATTACAGAGACCTCGTTTTTTGTTGTTTTCTTCATACGTCCATTTTTGTGACTTT TAACAAAGTCTGCACCTCCCAGTACTTTCTTTGGTACCTCTGCTTACTGCCTCTTGTGATGCCACTAGTC AGAATGCCTTGGAAAAGAGCTGTAGTTCTCCTAATGTTATGGTTTATAGGGCAGGCCATGTGGCTGGCTC CTGCCTATGTTCTAGAGTTTCAAGGAAAGAACACCTTTCTGTTTATTTGGTTAGCTGGTTTGTTCTTTCT TCTTATCAATTGTTCCATCCTGATTCAAATTATTTCCCATTACAAAGAAGAACCCCTGACAGAGAGAATC AAATATGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205766 representing NM_145167
Red=Cloning site Green=Tags(s) MGSTKHWGEWLLNLKVAPAGVFGVAFLARVALVFYGVFQDRTLHVRYTDIDYQVFTDAARFVTEGRSPYL RATYRYTPLLGWLLTPNIYLSELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAV SSRGNADSIVASLVLMVLYLIKKRLVACAAVFYGFAVHMKIYPVTYILPITLHLLPDRDNDKSLRQFRYT FQACLYELLKRLCNRAVLLFVAVAGLTFFALSFGFYYEYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYL TAESKWSFSLGIAAFLPQLILLSAVSFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLV RMPWKRAVVLLMLWFIGQAMWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHYKEEPLTERI KYD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145167 |
| ORF Size | 1269 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145167.3 |
| RefSeq Size | 4322 bp |
| RefSeq ORF | 1272 bp |
| Locus ID | 93183 |
| UniProt ID | Q9H3S5 |
| Domains | Mannosyl_trans |
| Protein Families | Transmembrane |
| Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways |
| Gene Summary | This gene encodes a transmembrane protein that is located in the endoplasmic reticulum and is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI)-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes a mannosyltransferase, GPI-MT-I, that transfers the first mannose to GPI on the lumenal side of the endoplasmic reticulum. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205766 | PIGM (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class M (PIGM) |
CNY 3,656.00 |
|
| RC205766L3 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class M (PIGM), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205766L4 | Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class M (PIGM), mGFP tagged |
CNY 5,890.00 |
|
| SC321702 | PIGM (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class M (PIGM) |
CNY 3,656.00 |
