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26S proteasome non ATPase regulatory subunit 12 (PSMD12) (NM_002816) Human Tagged ORF Clone
CAT＃: RG205680
- TrueORF®
PSMD12 (tGFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 (PSMD12), transcript variant 1
ORF Plasmid: DDK
"NM_002816" in other vectors (5)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | p55; Rpn5; STISS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205680 representing NM_002816
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGGCGGCTCGGAGCGGGCTGACGGGCGCATCGTCAAGATGGAGGTGGACTACAGCGCCACGG TGGATCAGCGCCTACCCGAGTGTGCGAAGCTAGCCAAGGAAGGAAGACTTCAAGAAGTCATTGAAACCCT TCTCTCTCTGGAAAAGCAGACTCGTACTGCTTCCGATATGGTATCGACATCCCGTATCTTAGTTGCAGTA GTGAAGATGTGCTATGAGGCTAAAGAATGGGATTTACTTAATGAAAATATTATGCTTTTGTCCAAAAGGC GGAGTCAGTTAAAACAAGCTGTTGCCAAAATGGTTCAACAGTGCTGTACTTATGTTGAGGAAATCACAGA CCTTCCTATCAAACTTCGATTAATTGATACTCTACGAATGGTTACCGAAGGCAAGATTTATGTTGAAATT GAGCGTGCGCGACTGACTAAAACATTAGCAACTATAAAAGAACAAAATGGTGATGTGAAAGAGGCAGCCT CCATTTTACAGGAGTTACAGGTGGAAACCTACGGGTCAATGGAAAAGAAAGAGCGAGTGGAATTTATTTT GGAGCAAATGAGGCTCTGCCTAGCTGTGAAGGATTACATTCGAACACAAATCATCAGCAAGAAAATTAAC ACCAAATTTTTCCAGGAAGAAAATACAGAGAAATTAAAGTTGAAGTACTATAATTTAATGATTCAGCTGG ATCAACATGAGGGATCCTATTTGTCTATTTGTAAGCACTACAGAGCAATATATGATACTCCCTGTATACA GGCAGAAAGTGAAAAATGGCAGCAGGCTCTGAAGAGTGTTGTACTCTATGTTATCCTGGCTCCTTTTGAC AATGAACAGTCAGATTTGGTTCACCGAATAAGTGGTGACAAGAAGTTAGAAGAAATTCCCAAATACAAGG ATCTTTTAAAGCTTTTTACCACAATGGAGTTGATGCGTTGGTCCACACTTGTTGAGGACTATGGAATGGA ATTAAGAAAAGGTTCCCTTGAGAGTCCTGCAACGGATGTTTTTGGTTCTACAGAGGAAGGTGAAAAAAGG TGGAAAGACTTGAAGAACAGAGTTGTTGAACATAATATTAGAATAATGGCCAAGTATTATACTCGGATAA CAATGAAAAGGATGGCACAGCTTCTGGATCTATCTGTTGATGAGTCCGAAGCCTTTCTCTCAAATCTAGT AGTTAACAAGACCATCTTTGCTAAAGTAGACAGATTAGCAGGAATTATCAACTTCCAGAGACCCAAGGAT CCAAATAATTTATTAAATGACTGGTCTCAGAAACTGAACTCATTAATGTCTCTGGTTAACAAAACTACGC ATCTCATAGCCAAAGAGGAGATGATACATAATCTACAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205680 representing NM_002816
Red=Cloning site Green=Tags(s) MADGGSERADGRIVKMEVDYSATVDQRLPECAKLAKEGRLQEVIETLLSLEKQTRTASDMVSTSRILVAV VKMCYEAKEWDLLNENIMLLSKRRSQLKQAVAKMVQQCCTYVEEITDLPIKLRLIDTLRMVTEGKIYVEI ERARLTKTLATIKEQNGDVKEAASILQELQVETYGSMEKKERVEFILEQMRLCLAVKDYIRTQIISKKIN TKFFQEENTEKLKLKYYNLMIQLDQHEGSYLSICKHYRAIYDTPCIQAESEKWQQALKSVVLYVILAPFD NEQSDLVHRISGDKKLEEIPKYKDLLKLFTTMELMRWSTLVEDYGMELRKGSLESPATDVFGSTEEGEKR WKDLKNRVVEHNIRIMAKYYTRITMKRMAQLLDLSVDESEAFLSNLVVNKTIFAKVDRLAGIINFQRPKD PNNLLNDWSQKLNSLMSLVNKTTHLIAKEEMIHNLQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002816 |
| ORF Size | 1368 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002816.5 |
| RefSeq Size | 1814 bp |
| RefSeq ORF | 1371 bp |
| Locus ID | 5718 |
| UniProt ID | O00232 |
| Domains | PCI |
| Protein Pathways | Proteasome |
| Gene Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205680 | PSMD12 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 (PSMD12), transcript variant 1 |
CNY 4,560.00 |
|
| RC205680L3 | Lenti-ORF clone of PSMD12 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 (PSMD12), transcript variant 1 |
CNY 6,460.00 |
|
| RC205680L4 | Lenti-ORF clone of PSMD12 (mGFP-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 (PSMD12), transcript variant 1 |
CNY 6,460.00 |
|
| SC118388 | PSMD12 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 (PSMD12), transcript variant 1 |
CNY 2,950.00 |
|
| SC320938 | PSMD12 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 (PSMD12), transcript variant 1 |
CNY 2,950.00 |
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