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RFXAP (NM_000538) Human Tagged ORF Clone
CAT＃: RG205569
- TrueORF®
RFXAP (tGFP-tagged) - Human regulatory factor X-associated protein (RFXAP)
ORF Plasmid: DDK
"NM_000538" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205569 representing NM_000538
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCGCAGAGTGTAGCGGAGGGCGCGGGGCCGGGCGCCGCCAGCGGCGTGCCCCACCCCGCGGCCC TAGCCCCGGCTGCGGCTCCCACCTTGGCGCCAGCCTCGGTGGCGGCCGCGGCCTCTCAATTCACCCTGCT AGTGATGCAACCCTGTGCTGGGCAGGACGAGGCTGCGGCCCCCGGGGGCAGCGTTGGGGCGGGCAAGCCC GTTAGGTACCTGTGCGAAGGGGCCGGGGATGGCGAAGAGGAGGCTGGGGAGGACGAGGCGGACCTGTTAG ACACTTCGGACCCTCCGGGGGGAGGCGAGAGCGCGGCTAGTTTGGAGGATCTAGAGGACGAGGAGACTCA CTCGGGGGGCGAGGGCAGCAGCGGGGGCGCCCGGAGGCGGGGCAGCGGTGGGGGCAGCATGAGCAAGACC TGCACCTACGAAGGCTGCAGCGAGACCACGAGCCAGGTGGCCAAGCAGCGCAAACCGTGGATGTGCAAGA AACACCGCAACAAGATGTACAAGGACAAGTATAAAAAGAAGAAGAGCGACCAGGCCCTGAACTGCGGTGG GACTGCCTCGACTGGCAGCGCGGGAAACGTCAAACTCGAGGAAAGTGCAGATAACATACTCTCCATTGTT AAACAAAGAACAGGATCTTTTGGGGATCGTCCTGCAAGACCTACTCTTTTAGAACAAGTGTTAAATCAAA AAAGACTGTCGTTACTAAGAAGTCCAGAAGTAGTGCAATTTTTACAGAAACAGCAACAGCTATTAAATCA GCAAGTTTTGGAGCAAAGACAACAGCAGTTTCCAGGAACATCAATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205569 representing NM_000538
Red=Cloning site Green=Tags(s) MEAQSVAEGAGPGAASGVPHPAALAPAAAPTLAPASVAAAASQFTLLVMQPCAGQDEAAAPGGSVGAGKP VRYLCEGAGDGEEEAGEDEADLLDTSDPPGGGESAASLEDLEDEETHSGGEGSSGGARRRGSGGGSMSKT CTYEGCSETTSQVAKQRKPWMCKKHRNKMYKDKYKKKKSDQALNCGGTASTGSAGNVKLEESADNILSIV KQRTGSFGDRPARPTLLEQVLNQKRLSLLRSPEVVQFLQKQQQLLNQQVLEQRQQQFPGTSM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000538 |
| ORF Size | 816 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000538.2, NP_000529.1 |
| RefSeq Size | 2825 bp |
| RefSeq ORF | 819 bp |
| Locus ID | 5994 |
| UniProt ID | O00287 |
| Protein Families | Transcription Factors |
| Protein Pathways | Antigen processing and presentation, Primary immunodeficiency |
| Gene Summary | Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated ankyrin-containing protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group D. Transcript variants utilizing different polyA signals have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205569 | RFXAP (Myc-DDK-tagged)-Human regulatory factor X-associated protein (RFXAP) |
CNY 2,400.00 |
|
| RC205569L3 | Lenti-ORF clone of RFXAP (Myc-DDK-tagged)-Human regulatory factor X-associated protein (RFXAP) |
CNY 5,890.00 |
|
| RC205569L4 | Lenti-ORF clone of RFXAP (mGFP-tagged)-Human regulatory factor X-associated protein (RFXAP) |
CNY 5,890.00 |
|
| SC310582 | RFXAP (untagged)-Human regulatory factor X-associated protein (RFXAP) |
CNY 6,270.00 |
