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DDX3Y (NM_004660) Human Tagged ORF Clone
CAT＃: RG205164
- TrueORF®
DDX3Y (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked (DDX3Y), transcript variant 2
ORF Plasmid: DDK
"NM_004660" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DBY |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205164 representing NM_004660
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCATGTGGTGGTGAAAAATGACCCTGAACTGGACCAGCAGCTTGCTAATCTGGACCTGAACTCTG AAAAACAGAGTGGAGGAGCAAGTACAGCGAGCAAAGGGCGCTATATACCTCCTCACTTAAGGAACAGAGA AGCATCTAAAGGATTCCATGATAAAGACAGTTCAGGTTGGAGTTGCAGCAAAGATAAGGATGCATATAGC AGTTTTGGGTCTCGAGATTCTAGAGGAAAGCCTGGTTATTTCAGTGAACGTGGAAGTGGATCAAGGGGAA GATTTGATGATCGTGGACGGAGTGACTATGATGGTATTGGCAATCGTGAAAGACCTGGCTTTGGCAGATT TGAACGGAGTGGACATAGTCGTTGGTGTGACAAGTCAGTTGAAGATGATTGGTCAAAACCACTTCCACCA AGTGAACGCTTGGAGCAAGAACTGTTTTCTGGAGGAAACACGGGGATTAACTTTGAGAAATATGATGATA TACCAGTAGAGGCAACCGGCAGTAACTGTCCTCCACATATTGAGAATTTTAGCGATATTGACATGGGAGA AATTATCATGGGGAACATTGAACTTACTCGCTATACTCGTCCTACTCCAGTGCAAAAACATGCCATTCCT ATTATTAAGGGAAAAAGAGACTTAATGGCTTGTGCCCAAACAGGATCTGGGAAAACTGCAGCATTTCTTT TACCCATACTGAGTCAGATATATACAGATGGTCCAGGAGAAGCTTTGAAGGCTGTGAAGGAAAATGGAAG GTATGGGCGCCGCAAACAATATCCAATATCCTTGGTTTTAGCCCCAACAAGAGAATTGGCTGTACAGATC TATGAGGAAGCCAGAAAATTTTCCTACCGATCTAGAGTTCGTCCTTGTGTAGTTTATGGTGGTGCTGATA TTGGTCAGCAGATTCGGGACTTAGAACGTGGATGCCACTTGTTAGTAGCCACTCCAGGACGTCTAGTGGA TATGATGGAAAGAGGAAAGATTGGATTAGACTTCTGCAAGTACTTAGTGTTGGATGAAGCTGATAGGATG CTGGATATGGGATTTGAACCTCAGATACGTCGTATAGTTGAACAAGATACTATGCCACCAAAGGGCGTTC GTCACACCATGATGTTTAGTGCTACTTTTCCTAAGGAAATACAGATGCTTGCTCGTGACTTTTTGGATGA ATATATCTTTTTGGCTGTAGGCAGAGTAGGCTCTACCTCTGAGAACATCACACAGAAAGTAGTTTGGGTG GAAGACTTAGATAAACGGTCATTTCTACTGGACATTTTAGGTGCAACAGGGAGTGATTCACTTACTTTAG TGTTTGTGGAGACCAAAAAGGGAGCAGATTCCCTGGAGGATTTCTTATACCATGAAGGATATGCTTGTAC TAGTATTCATGGAGACCGGTCACAGAGAGATCGAGAGGAGGCCCTTCACCAGTTTCGCTCAGGAAAAAGC CCAATTCTAGTGGCTACAGCTGTGGCAGCACGAGGACTAGACATTTCAAATGTGAGACATGTTATCAATT TTGATTTGCCAAGTGATATTGAAGAATATGTGCATCGTATTGGCCGTACAGGACGTGTAGGAAACCTGGG CCTTGCCACCTCATTCTTTAATGAAAAAAATATGAATATTACAAAGGATTTGTTGGATCTTCTTGTAGAA GCTAAACAAGAAGTGCCTTCTTGGTTGGAAAATATGGCTTATGAACACCACTACAAGGGTGGCAGTCGTG GACGATCTAAAAGTAATAGATTCAGTGGAGGATTTGGTGCCAGAGACTATCGACAAAGTAGTGGTTCCAG CAGTTCTGGCTTTGGTGCTAGTCGCGGAAGCAGCAGCCGCAGTGGTGGAGGTGGTTACGGCAACAGCAGA GGATTTGGTGGAGGTGGCTATGGAGGCTTCTACAATAGTGATGGATATGGAGGAAATTATAACTCCCAGG GGGTTGACTGGTGGGGCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205164 representing NM_004660
Red=Cloning site Green=Tags(s) MSHVVVKNDPELDQQLANLDLNSEKQSGGASTASKGRYIPPHLRNREASKGFHDKDSSGWSCSKDKDAYS SFGSRDSRGKPGYFSERGSGSRGRFDDRGRSDYDGIGNRERPGFGRFERSGHSRWCDKSVEDDWSKPLPP SERLEQELFSGGNTGINFEKYDDIPVEATGSNCPPHIENFSDIDMGEIIMGNIELTRYTRPTPVQKHAIP IIKGKRDLMACAQTGSGKTAAFLLPILSQIYTDGPGEALKAVKENGRYGRRKQYPISLVLAPTRELAVQI YEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRM LDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWV EDLDKRSFLLDILGATGSDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKS PILVATAVAARGLDISNVRHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNEKNMNITKDLLDLLVE AKQEVPSWLENMAYEHHYKGGSRGRSKSNRFSGGFGARDYRQSSGSSSSGFGASRGSSSRSGGGGYGNSR GFGGGGYGGFYNSDGYGGNYNSQGVDWWGN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004660 |
| ORF Size | 1980 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004660.5 |
| RefSeq Size | 4488 bp |
| RefSeq ORF | 1983 bp |
| Locus ID | 8653 |
| UniProt ID | O15523 |
| Domains | DEAD, helicase_C |
| Protein Pathways | RIG-I-like receptor signaling pathway |
| Gene Summary | The protein encoded by this gene is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of this gene is not complemented by DDX3X. Mutations in this gene result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both this gene and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205164 | DDX3Y (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked (DDX3Y), transcript variant 2 |
CNY 3,990.00 |
|
| RC205164L3 | Lenti-ORF clone of DDX3Y (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked (DDX3Y), transcript variant 2 |
CNY 5,890.00 |
|
| RC205164L4 | Lenti-ORF clone of DDX3Y (mGFP-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked (DDX3Y), transcript variant 2 |
CNY 5,890.00 |
|
| SC117258 | DDX3Y (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked (DDX3Y), transcript variant 2 |
CNY 4,920.00 |
