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Helicase SKI2W (SKIV2L) (NM_006929) Human Tagged ORF Clone
CAT＃: RG204893
- TrueORF®
SKIV2L (tGFP-tagged) - Human superkiller viralicidic activity 2-like (S. cerevisiae) (SKIV2L)
ORF Plasmid: DDK
"NM_006929" in other vectors (4)
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CNY 10,550.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 170A; DDX13; HLP; SKI2; SKI2W; SKIV2; SKIV2L1; THES2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG204893 representing NM_006929
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGAGACAGAGCGACTTGTGCTACCCCCTCCAGATCCCCTGGACCTACCCCTTCGGGCCGTGGAGC TCGGATGCACGGGGCACTGGGAGCTGCTGAACTTGCCTGGAGCTCCAGAGAGTAGCCTTCCCCATGGCCT CCCTCCTTGTGCCCCAGATCTGCAGCAAGAAGCAGAACAGTTGTTTCTGTCATCCCCAGCCTGGCTGCCT CTGCATGGTGTGGAGCACTCAGCCCGAAAATGGCAGAGGAAGACGGATCCCTGGTCTCTTTTGGCTGTGC TGGGAGCCCCAGTCCCATCCGACCTACAGGCCCAAAGACACCCAACCACAGGCCAGATACTGGGTTACAA AGAGGTCTTGCTGGAGAACACAAATCTCTCGGCTACAACCTCCTTGTCTCTTCGCCGGCCTCCAGGGCCA GCCTCCCAGTCCTTATGGGGAAATCCAACTCGGTATCCCTTCTGGCCAGGGGGGATGGATGAACCCACCA TAACAGATCTGAACACACGGGAGGAGGCTGAGGAGGAGATAGACTTTGAGAAAGATCTTCTTACTATTCC ACCTGGTTTCAAGAAAGGCATGGACTTTGCACCAAAAGATTGTCCAACTCCAGCTCCTGGACTACTAAGC CTTAGCTGTCTGTTGGAGCCTCTGGATTTGGGTGGGGGTGACGAGGATGAGAATGAGGCAGTGGGACAGC CAGGAGGTCCCAGAGGGGACACTGTTTCAGCCTCTCCCTGCAGTGCTCCCCTGGCCCGAGCAAGCAGCTT GGAAGACCTAGTGTTGAAGGAAGCGTCCACAGCTGTATCCACCCCAGAGGCCCCAGAGCCTCCATCTCAG GAGCAGTGGGCCATCCCTGTGGACGCCACCTCCCCTGTTGGTGATTTCTATCGCCTCATTCCCCAGCCAG CCTTCCAGTGGGCATTTGAGCCAGATGTGTTTCAGAAACAGGCCATCCTGCACTTGGAACGGCATGACTC TGTCTTTGTCGCAGCTCACACATCTGCAGGAAAAACAGTTGTGGCTGAATATGCCATTGCCCTGGCCCAG AAACACATGACACGCACCATCTACACTTCGCCCATCAAGGCCCTGAGCAACCAGAAGTTCCGGGACTTCC GAAACACATTCGGGGATGTGGGGCTGCTCACCGGGGATGTACAGCTGCATCCGGAGGCCTCCTGCCTCAT CATGACCACAGAGATCCTTCGCTCCATGCTGTACAGTGGCTCAGATGTTATTCGGGACCTGGAGTGGGTC ATCTTTGATGAGGTTCACTATATCAACGATGTCGAGCGTGGGGTCGTGTGGGAGGAGGTGCTTATCATGC TACCTGACCACGTTTCTATCATCCTTCTGAGTGCCACCGTCCCCAACGCCCTTGAGTTTGCTGACTGGAT TGGGCGGCTGAAGCGTCGTCAGATCTATGTGATTAGCACTGTAACCCGCCCCGTGCCCCTGGAGCACTAT CTTTTCACAGGGAACAGCTCCAAGACCCAGGGGGAGCTCTTTTTGTTGCTGGACTCCCGAGGAGCCTTCC ATACAAAAGGGTACTATGCAGCTGTGGAGGCCAAGAAGGAGAGAATGAGCAAACACGCCCAGACCTTTGG GGCCAAGCAGCCCACACATCAGGGGGGCCCTGCACAGGACCGCGGAGTGTACCTGTCCCTCCTGGCCTCC CTCCGCACACGTGCCCAGTTGCCCGTGGTGGTGTTCACCTTCTCCCGGGGCCGCTGTGATGAGCAGGCCT CAGGCCTCACCTCCCTTGACCTCACCACCAGTTCGGAGAAGAGCGAGATCCACCTCTTCCTGCAGCGCTG CCTTGCTCGCCTCCGTGGCTCTGACCGCCAGCTGCCCCAGGTCCTGCACATGTCAGAGCTCCTGAATCGC GGCCTGGGTGTGCACCATAGCGGCATCCTGCCCATCCTCAAGGAGATCGTGGAGATGCTCTTCAGCCGTG GCCTGGTCAAGGTCTTGTTTGCCACAGAGACCTTTGCCATGGGAGTAAACATGCCTGCTCGTACAGTAGT GTTTGACTCCATGCGCAAACACGATGGCTCCACCTTCCGGGACCTGCTCCCTGGGGAGTATGTGCAGATG GCAGGCCGGGCAGGGCGGAGGGGCCTGGACCCCACAGGCACCGTTATCCTGCTCTGCAAGGGCCGAGTGC CCGAGATGGCAGACCTGCACCGCATGATGATGGGGAAGCCGTCCCAGCTGCAGTCCCAGTTCCGCCTCAC GTACACTATGATCCTCAACTTGCTGCGAGTGGATGCCCTCAGGGTGGAGGACATGATGAAGAGGAGCTTC TCTGAGTTTCCCTCCCGCAAAGACAGCAAGGCCCATGAACAGGCCCTGGCTGAACTGACCAAGAGGCTGG GAGCTTTGGAGGAGCCTGACATGACTGGCCAACTGGTCGACCTGCCTGAATATTACAGCTGGGGGGAGGA ACTGACAGAGACCCAGCACATGATCCAGCGACGCATCATGGAGTCTGTGAACGGGCTGAAGTCTCTCTCA GCAGGAAGGGTGGTGGTTGTGAAGAATCAGGAGCATCACAACGCATTGGGAGTGATCCTACAGGTCTCCT CGAACTCCACCAGCAGAGTATTCACAACCCTGGTCTTGTGTGATAAGCCCTTGTCCCAGGACCCACAGGA CAGGGGGCCAGCCACTGCAGAGGTGCCCTATCCAGATGACCTCGTGGGATTCAAGCTGTTCCTGCCTGAA GGGCCTTGTGACCACACCGTGGTCAAGCTCCAGCCAGGAGATATGGCTGCCATCACCACCAAGGTGCTCC GGGTGAATGGGGAGAAGATCTTGGAGGACTTCAGCAAGAGGCAGCAGCCAAAATTCAAGAAGGATCCTCC CCTTGCAGCCGTGACCACTGCTGTCCAGGAACTGCTGCGTCTGGCTCAGGCCCACCCAGCCGGACCTCCC ACCCTCGACCCTGTCAATGACCTGCAGCTCAAAGATATGTCAGTTGTAGAGGGTGGGCTCCGGGCCCGGA AGCTGGAGGAGCTGATCCAGGGGGCTCAGTGTGTACACAGCCCCCGTTTTCCTGCCCAGTACCTGAAGCT GCGGGAGCGAATGCAGATACAGAAGGAGATGGAGCGGCTGCGCTTCCTACTGTCGGATCAGTCATTGCTG CTGCTTCCTGAGTACCATCAGCGAGTAGAGGTGCTCCGAACCCTGGGTTACGTGGACGAGGTGGGCACTG TGAAGCTGGCAGGGCGGGTGGCTTGTGCCATGAGCAGCCATGAGTTGCTCCTCACTGAGCTCATGTTTGA CAATGCACTGAGCACCCTGCGGCCTGAGGAGATTGCTGCCTTGCTCTCTGGCCTGGTCTGCCAGAGCCCT GGGGACGCTGGGGATCAGCTCCCAAACACCCTCAAGCAGGGAATAGAACGTGTCCGGGCTGTGGCCAAGC GGATTGGTGAGGTCCAGGTGGCTTGTGGCCTGAACCAGACGGTGGAGGAATTTGTGGGGGAGCTGAATTT TGGGCTGGTTGAGGTTGTATATGAGTGGGCCCGGGGCATGCCCTTCTCCGAGTTGGCAGGGCTCTCAGGG ACCCCTGAGGGCCTGGTGGTCCGCTGCATTCAGCGCCTGGCTGAGATGTGTCGCTCACTGCGGGGGGCAG CCCGCCTGGTAGGAGAGCCTGTGCTGGGTGCCAAGATGGAGACAGCGGCTACCTTGCTACGGCGGGACAT CGTATTTGCGGCCAGCCTCTACACCCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204893 representing NM_006929
Red=Cloning site Green=Tags(s) MMETERLVLPPPDPLDLPLRAVELGCTGHWELLNLPGAPESSLPHGLPPCAPDLQQEAEQLFLSSPAWLP LHGVEHSARKWQRKTDPWSLLAVLGAPVPSDLQAQRHPTTGQILGYKEVLLENTNLSATTSLSLRRPPGP ASQSLWGNPTRYPFWPGGMDEPTITDLNTREEAEEEIDFEKDLLTIPPGFKKGMDFAPKDCPTPAPGLLS LSCLLEPLDLGGGDEDENEAVGQPGGPRGDTVSASPCSAPLARASSLEDLVLKEASTAVSTPEAPEPPSQ EQWAIPVDATSPVGDFYRLIPQPAFQWAFEPDVFQKQAILHLERHDSVFVAAHTSAGKTVVAEYAIALAQ KHMTRTIYTSPIKALSNQKFRDFRNTFGDVGLLTGDVQLHPEASCLIMTTEILRSMLYSGSDVIRDLEWV IFDEVHYINDVERGVVWEEVLIMLPDHVSIILLSATVPNALEFADWIGRLKRRQIYVISTVTRPVPLEHY LFTGNSSKTQGELFLLLDSRGAFHTKGYYAAVEAKKERMSKHAQTFGAKQPTHQGGPAQDRGVYLSLLAS LRTRAQLPVVVFTFSRGRCDEQASGLTSLDLTTSSEKSEIHLFLQRCLARLRGSDRQLPQVLHMSELLNR GLGVHHSGILPILKEIVEMLFSRGLVKVLFATETFAMGVNMPARTVVFDSMRKHDGSTFRDLLPGEYVQM AGRAGRRGLDPTGTVILLCKGRVPEMADLHRMMMGKPSQLQSQFRLTYTMILNLLRVDALRVEDMMKRSF SEFPSRKDSKAHEQALAELTKRLGALEEPDMTGQLVDLPEYYSWGEELTETQHMIQRRIMESVNGLKSLS AGRVVVVKNQEHHNALGVILQVSSNSTSRVFTTLVLCDKPLSQDPQDRGPATAEVPYPDDLVGFKLFLPE GPCDHTVVKLQPGDMAAITTKVLRVNGEKILEDFSKRQQPKFKKDPPLAAVTTAVQELLRLAQAHPAGPP TLDPVNDLQLKDMSVVEGGLRARKLEELIQGAQCVHSPRFPAQYLKLRERMQIQKEMERLRFLLSDQSLL LLPEYHQRVEVLRTLGYVDEVGTVKLAGRVACAMSSHELLLTELMFDNALSTLRPEEIAALLSGLVCQSP GDAGDQLPNTLKQGIERVRAVAKRIGEVQVACGLNQTVEEFVGELNFGLVEVVYEWARGMPFSELAGLSG TPEGLVVRCIQRLAEMCRSLRGAARLVGEPVLGAKMETAATLLRRDIVFAASLYTQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006929 |
| ORF Size | 3738 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006929.4, NP_008860.4 |
| RefSeq Size | 4187 bp |
| RefSeq ORF | 3741 bp |
| Locus ID | 6499 |
| UniProt ID | Q15477 |
| Domains | DEAD, helicase_C |
| Protein Pathways | RNA degradation |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a human homologue of yeast SKI2 and may be involved in antiviral activity by blocking translation of poly(A) deficient mRNAs. This gene is located in the class III region of the major histocompatibility complex. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204893 | SKIV2L (Myc-DDK-tagged)-Human superkiller viralicidic activity 2-like (S. cerevisiae) (SKIV2L) |
CNY 8,680.00 |
|
| RC204893L3 | Lenti ORF clone of Human superkiller viralicidic activity 2-like (S. cerevisiae) (SKIV2L), Myc-DDK-tagged |
CNY 11,500.00 |
|
| RC204893L4 | Lenti ORF clone of Human superkiller viralicidic activity 2-like (S. cerevisiae) (SKIV2L), mGFP tagged |
CNY 11,500.00 |
|
| SC319532 | SKIV2L (untagged)-Human superkiller viralicidic activity 2-like (S. cerevisiae) (SKIV2L) |
CNY 8,680.00 |
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