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NCE2 (UBE2F) (NM_080678) Human Tagged ORF Clone
CAT＃: RG203942
- TrueORF®
UBE2F (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 1
ORF Plasmid: DDK
"NM_080678" in other vectors (4)
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CNY 4,000.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | NCE2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203942 representing NM_080678
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAACGCTAGCAAGTAAACTGAAGCGTGACGATGGTCTCAAAGGGTCCCGGACGGCAGCCACAGCGT CCGACTCGACTCGGAGGGTTTCTGTGAGAGACAAATTGCTTGTTAAAGAGGTTGCAGAACTTGAAGCTAA TTTACCTTGTACATGTAAAGTGCATTTTCCTGATCCAAACAAGCTTCATTGTTTTCAGCTAACAGTAACC CCAGATGAGGGTTACTACCAGGGTGGAAAATTTCAGTTTGAAACTGAAGTTCCCGATGCGTACAACATGG TGCCTCCCAAAGTGAAATGCCTGACCAAGATCTGGCACCCCAACATCACAGAGACAGGGGAAATATGTCT GAGTTTATTGAGAGAACATTCAATTGATGGCACTGGCTGGGCTCCCACAAGAACATTAAAGGATGTCGTT TGGGGATTAAACTCTTTGTTTACTGATCTTTTGAATTTTGATGATCCACTGAATATTGAAGCTGCAGAAC ATCATTTGCGGGACAAGGAGGACTTCCGGAATAAAGTGGATGACTACATCAAACGTTATGCCAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203942 representing NM_080678
Red=Cloning site Green=Tags(s) MLTLASKLKRDDGLKGSRTAATASDSTRRVSVRDKLLVKEVAELEANLPCTCKVHFPDPNKLHCFQLTVT PDEGYYQGGKFQFETEVPDAYNMVPPKVKCLTKIWHPNITETGEICLSLLREHSIDGTGWAPTRTLKDVV WGLNSLFTDLLNFDDPLNIEAAEHHLRDKEDFRNKVDDYIKRYAR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080678 |
| ORF Size | 555 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_080678.3 |
| RefSeq Size | 1366 bp |
| RefSeq ORF | 558 bp |
| Locus ID | 140739 |
| UniProt ID | Q969M7 |
| Domains | UBCc |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203942 | UBE2F (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 1 |
CNY 2,400.00 |
|
| RC203942L3 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203942L4 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC120332 | UBE2F (untagged)-Human ubiquitin-conjugating enzyme E2F (putative) (UBE2F), transcript variant 1 |
CNY 2,400.00 |
