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CDC34 (NM_004359) Human Tagged ORF Clone
CAT＃: RG203517
- TrueORF®
CDC34 (tGFP-tagged) - Human cell division cycle 34 homolog (S. cerevisiae) (CDC34)
ORF Plasmid: DDK
"NM_004359" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | E2-CDC34; UBC3; UBCH3; UBE2R1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203517 representing NM_004359
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCGGCCGCTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGAGCTCAAGGGGCTGCAGGAAGAGC CGGTCGAGGGATTCCGCGTGACACTGGTGGACGAGGGCGATCTATACAACTGGGAGGTGGCCATCTTCGG GCCCCCCAACACCTACTACGAGGGCGGCTACTTCAAGGCGCGCCTCAAGTTCCCCATCGACTACCCATAC TCTCCACCAGCCTTTCGGTTCCTGACCAAGATGTGGCACCCTAACATCTACGAGACGGGGGACGTGTGTA TCTCCATCCTCCACCCGCCGGTGGACGACCCCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCCCAC GCAGAACGTCAGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACGAGCCCAACACCTTCTCGCCCGCA AACGTGGACGCCTCCGTGATGTACAGGAAGTGGAAAGAGAGCAAGGGGAAGGATCGGGAGTACACAGACA TCATCCGGAAGCAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGCGTGAAGGTGCCCACCACGCT GGCCGAGTACTGCGTGAAGACCAAGGCGCCGGCGCCCGACGAGGGCTCAGACCTCTTCTACGACGACTAC TACGAGGACGGCGAGGTGGAGGAGGAGGCCGACAGCTGCTTCGGGGACGATGAGGATGACTCTGGCACGG AGGAGTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203517 representing NM_004359
Red=Cloning site Green=Tags(s) MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPY SPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPA NVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDY YEDGEVEEEADSCFGDDEDDSGTEES TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004359 |
| ORF Size | 708 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004359.2 |
| RefSeq Size | 1462 bp |
| RefSeq ORF | 711 bp |
| Locus ID | 997 |
| UniProt ID | P49427 |
| Domains | UBCc |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203517 | CDC34 (Myc-DDK-tagged)-Human cell division cycle 34 homolog (S. cerevisiae) (CDC34) |
CNY 2,400.00 |
|
| RC203517L1 | Lenti ORF clone of Human cell division cycle 34 homolog (S. cerevisiae) (CDC34), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC203517L2 | Lenti ORF clone of Human cell division cycle 34 homolog (S. cerevisiae) (CDC34), mGFP tagged |
CNY 5,890.00 |
|
| RC203517L3 | Lenti ORF clone of Human cell division cycle 34 homolog (S. cerevisiae) (CDC34), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203517L4 | Lenti ORF clone of Human cell division cycle 34 homolog (S. cerevisiae) (CDC34), mGFP tagged |
CNY 5,890.00 |
|
| SC103113 | CDC34 (untagged)-Human cell division cycle 34 homolog (S. cerevisiae) (CDC34) |
CNY 2,400.00 |
