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UBC (NM_021009) Human Tagged ORF Clone
CAT＃: RG203292
- TrueORF®
UBC (tGFP-tagged) - Human ubiquitin C (UBC). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_021009" in other vectors (4)
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CNY 9,128.00
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热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HMG20 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203292 ORF sequence, codon optimized.
Due to the complexity of NM_021009, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAATCTTTGTGAAGACTCTGACGGGGAAAACCATAACACTGGAAGTCGAACCTTCTGATACTATAG AAAATGTCAAGGCAAAGATTCAGGATAAAGAAGGTATCCCACCGGATCAGCAGCGACTGATCTTCGCAGG TAAGCAACTGGAGGACGGACGCACTTTGTCAGATTATAACATTCAAAAGGAAAGCACGCTCCACCTGGTC TTGCGGCTGCGGGGAGGCATGCAGATCTTTGTGAAAACATTGACCGGCAAGACAATTACCCTCGAGGTGG AACCCAGCGACACCATTGAGAATGTCAAGGCCAAGATTCAGGACAAGGAGGGAATTCCTCCAGATCAGCA GAGACTCATCTTCGCCGGGAAGCAGCTGGAAGACGGACGCACTCTGTCTGACTACAATATCCAGAAGGAA TCAACTCTCCACCTCGTCCTGAGGCTGAGAGGAGGAATGCAGATATTTGTAAAAACCTTGACGGGCAAAA CAATAACCCTTGAAGTCGAACCCTCTGATACAATAGAGAACGTGAAAGCCAAAATTCAGGACAAGGAGGG GATTCCGAGCGATCAGCAGCGCTTGATCTTTGCTGGGAAGCAGCTCGAGGACGGTCGAACTCTTAGTGAC TATAATATCCAGAAGGAGAGCACCCTTCACCTGGTCCTGAGGCTGCGAGGGGGAATGCAGATTTTTGTGA AGACCCTGACCGGCAAAACAATTACACTTGAGGTGGAACCTAGCGACACTATCGAGAATGTAAAAGCCAA AATACAGGACAAGGAAGGAATACCTCCTGACCAGCAGAGGCTTATTTTTGCAGGGAAGCAACTGGAGGAT GGACGGACTCTGAGTGACTATAATATACAGAAAGAATCCACCCTCCACCTGGTACTCAGACTCCGCGGCG GGATGCAGATTTTTGTAAAGACCCTCACCGGGAAGACTATCACTCTGGAGGTCGAACCTTCCGACACTAT CGAGAACGTAAAAGCCAAGATCCAGGATAAGGAGGGAATTCCCCCAGACCAGCAGCGGTTGATTTTTGCA GGAAAACAACTTGAGGACGGACGCACCTTGTCTGACTACAATATCCAAAAGGAGTCAACCTTGCATCTTG TCCTCAGACTGCGCGGGGGAATGCAGATTTTCGTGAAGACCCTCACTGGCAAAACAATCACGCTCGAGGT GGAGCCTTCCGATACTATAGAGAATGTCAAGGCAAAGATACAGGATAAAGAAGGCATCCCCCCTGACCAG CAGCGGCTGATCTTTGCGGGAAAGCAGCTCGAGGATGGCCGAACTCTGTCAGATTACAATATTCAGAAAG AATCCACCCTTCATCTGGTGCTTCGGCTTAGAGGAGGAATGCAAATCTTTGTGAAGACCCTTACGGGAAA GACTATTACGCTGGAGGTGGAACCCAGCGATACGATAGAAAACGTGAAGGCAAAAATTCAAGATAAAGAA GGAATCCCTCCCGACCAGCAGCGGCTTATTTTCGCTGGCAAGCAGCTTGAGGACGGGCGCACACTGTCTG ATTACAATATTCAAAAGGAAAGCACCCTGCATCTGGTACTGCGGCTGAGGGGCGGTATGCAGATTTTCGT CAAAACGCTCACCGGCAAAACGATAACACTGGAAGTTGAACCCTCAGACACGATTGAGAACGTAAAGGCA AAAATTCAAGACAAGGAGGGAATTCCACCGGATCAGCAGCGCCTGATTTTTGCCGGCAAGCAGCTGGAGG ATGGTCGGACCCTCAGTGATTACAATATTCAGAAGGAGTCCACACTCCATCTGGTGCTGAGGTTGAGGGG TGGAATGCAGATTTTTGTCAAGACTCTCACCGGCAAGACTATTACCCTCGAAGTGGAGCCCTCCGATACC ATCGAGAATGTGAAGGCTAAAATCCAGGACAAGGAGGGTATCCCACCTGATCAACAGAGGCTTATCTTCG CTGGCAAACAGCTGGAGGACGGCCGCACCCTGAGCGACTATAATATCCAAAAGGAGTCAACTCTGCACCT TGTACTGCGCCTCAGAGGGGGAGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203292 representing NM_021009
Red=Cloning site Green=Tags(s) MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV LRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKE STLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPSDQQRLIFAGKQLEDGRTLSD YNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLED GRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA GKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKE GIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKA KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDT IENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021009 |
| ORF Size | 2055 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021009.5, NP_066289.2 |
| RefSeq Size | 2193 bp |
| RefSeq ORF | 2058 bp |
| Locus ID | 7316 |
| UniProt ID | P0CG48 |
| Domains | UBQ |
| Protein Families | Druggable Genome |
| Protein Pathways | PPAR signaling pathway |
| Gene Summary | This gene represents a ubiquitin gene, ubiquitin C. The encoded protein is a polyubiquitin precursor. Conjugation of ubiquitin monomers or polymers can lead to various effects within a cell, depending on the residues to which ubiquitin is conjugated. Ubiquitination has been associated with protein degradation, DNA repair, cell cycle regulation, kinase modification, endocytosis, and regulation of other cell signaling pathways. [provided by RefSeq, Aug 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203292 | UBC (Myc-DDK-tagged)-Human ubiquitin C (UBC). Note: ORF is codon optimized |
CNY 7,528.00 |
|
| RC203292L3 | Lenti-ORF clone of UBC (Myc-DDK-tagged)-Human ubiquitin C (UBC). Note: ORF is codon optimized |
CNY 7,700.00 |
|
| RC203292L4 | Lenti-ORF clone of UBC (mGFP-tagged)-Human ubiquitin C (UBC). Note: ORF is codon optimized |
CNY 7,700.00 |
|
| SC108441 | UBC (untagged)-Human ubiquitin C (UBC) |
CNY 7,536.00 |
