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BHMT (NM_001713) Human Tagged ORF Clone
CAT＃: RG203148
- TrueORF®
BHMT (tGFP-tagged) - Human betaine--homocysteine S-methyltransferase (BHMT)
ORF Plasmid: DDK
"NM_001713" in other vectors (6)
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CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BHMT1; HEL-S-61p |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG203148 representing NM_001713
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACCCGTTGGGGGCAAAAAGGCCAAGAAGGGCATCCTAGAACGTTTAAATGCTGGAGAGATTGTGA TTGGAGATGGAGGGTTTGTCTTTGCACTGGAGAAGAGGGGCTACGTAAAGGCAGGACCCTGGACTCCTGA AGCTGCTGTGGAGCACCCAGAAGCAGTTCGCCAGCTTCATCGAGAGTTCCTCAGAGCTGGCTCAAACGTC ATGCAGACCTTCACCTTCTATGCGAGTGAAGACAAGCTGGAGAACAGGGGCAACTATGTCTTAGAGAAGA TATCTGGGCAGGAAGTCAATGAAGCTGCTTGCGACATCGCCCGACAAGTGGCTGATGAAGGAGATGCTTT GGTAGCAGGAGGAGTGAGTCAGACACCTTCATACCTTAGCTGCAAGAGTGAAACTGAAGTCAAAAAAGTA TTTCTGCAACAGTTAGAGGTCTTTATGAAGAAGAACGTGGACTTCTTGATTGCAGAGTATTTTGAACACG TTGAAGAAGCTGTGTGGGCAGTTGAAACCTTGATAGCATCCGGTAAACCTGTGGCAGCAACCATGTGCAT TGGCCCAGAAGGAGATTTGCATGGCGTGCCCCCCGGCGAGTGTGCAGTGCGCCTGGTGAAAGCAGGAGCA TCCATCATTGGTGTGAACTGCCACTTTGACCCCACCATTAGTTTAAAAACAGTGAAGCTCATGAAGGAGG GCTTGGAGGCTGCCCGACTGAAAGCTCACCTGATGAGCCAGCCCTTGGCTTACCACACTCCTGACTGCAA CAAGCAGGGATTCATCGATCTCCCAGAATTCCCATTTGGACTGGAACCCAGAGTTGCCACCAGATGGGAT ATTCAAAAATACGCCAGAGAGGCCTACAACCTGGGGGTCAGGTACATTGGCGGGTGCTGTGGATTTGAGC CCTACCACATCAGGGCAATTGCAGAGGAGCTGGCCCCAGAAAGGGGCTTTTTGCCACCAGCTTCAGAAAA ACATGGCAGCTGGGGAAGTGGTTTGGACATGCACACCAAACCCTGGGTTAGAGCAAGGGCCAGGAAGGAA TACTGGGAGAATCTTCGGATAGCCTCAGGCCGGCCATACAACCCTTCAATGTCAAAGCCAGATGGCTGGG GAGTGACCAAAGGAACAGCCGAGCTGATGCAGCAGAAAGAAGCCACAACTGAGCAGCAGCTGAAAGAGCT CTTTGAAAAACAAAAATTCAAATCACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203148 representing NM_001713
Red=Cloning site Green=Tags(s) MPPVGGKKAKKGILERLNAGEIVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNV MQTFTFYASEDKLENRGNYVLEKISGQEVNEAACDIARQVADEGDALVAGGVSQTPSYLSCKSETEVKKV FLQQLEVFMKKNVDFLIAEYFEHVEEAVWAVETLIASGKPVAATMCIGPEGDLHGVPPGECAVRLVKAGA SIIGVNCHFDPTISLKTVKLMKEGLEAARLKAHLMSQPLAYHTPDCNKQGFIDLPEFPFGLEPRVATRWD IQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWVRARARKE YWENLRIASGRPYNPSMSKPDGWGVTKGTAELMQQKEATTEQQLKELFEKQKFKSQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001713 |
| ORF Size | 1218 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001713.3 |
| RefSeq Size | 2436 bp |
| RefSeq ORF | 1221 bp |
| Locus ID | 635 |
| UniProt ID | Q93088 |
| Domains | S-methyl_trans |
| Protein Pathways | Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways |
| Gene Summary | This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203148 | BHMT (Myc-DDK-tagged)-Human betaine--homocysteine S-methyltransferase (BHMT) |
CNY 3,656.00 |
|
| RC203148L1 | Lenti ORF clone of Human betaine--homocysteine S-methyltransferase (BHMT), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203148L2 | Lenti ORF clone of Human betaine--homocysteine S-methyltransferase (BHMT), mGFP tagged |
CNY 5,890.00 |
|
| RC203148L3 | Lenti ORF clone of Human betaine--homocysteine S-methyltransferase (BHMT), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203148L4 | Lenti ORF clone of Human betaine--homocysteine S-methyltransferase (BHMT), mGFP tagged |
CNY 5,890.00 |
|
| SC110837 | BHMT (untagged)-Human betaine--homocysteine S-methyltransferase (BHMT) |
CNY 3,656.00 |
