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XPB (ERCC3) (NM_000122) Human Tagged ORF Clone
CAT＃: RG202425
- TrueORF®
ERCC3 (tGFP-tagged) - Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3)
ORF Plasmid: DDK
"NM_000122" in other vectors (7)
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CNY 6,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BTF2; GTF2H; RAD25; Ssl2; TFIIH; TTD2; XPB |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG202425 representing NM_000122
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAAAAGAGACCGAGCGGACCGCGACAAGAAGAAATCCAGGAAGCGGCACTATGAGGATGAAGAGG ATGATGAAGAGGACGCCCCGGGGAACGACCCTCAGGAAGCGGTTCCCTCGGCGGCGGGGAAGCAGGTGGA TGAGTCAGGCACCAAAGTGGATGAATATGGAGCCAAGGACTACAGGCTGCAAATGCCGCTGAAGGACGAC CACACCTCCAGGCCCCTCTGGGTGGCTCCCGATGGCCATATCTTCTTGGAAGCCTTCTCTCCAGTTTACA AATATGCCCAAGACTTCTTGGTGGCTATTGCAGAGCCAGTGTGCCGACCAACCCATGTGCATGAGTACAA ACTAACTGCCTACTCCTTGTATGCAGCTGTCAGCGTTGGGCTGCAAACCAGTGACATCACCGAGTACCTC AGGAAGCTCAGCAAGACTGGAGTCCCTGATGGAATTATGCAGTTTATTAAGTTGTGTACTGTCAGCTATG GAAAAGTCAAGCTGGTCTTGAAGCACAACAGATACTTCGTTGAAAGTTGCCACCCTGATGTAATCCAGCA TCTTCTCCAGGACCCCGTGATCCGAGAATGCCGCTTAAGAAACTCTGAAGGGGAGGCCACTGAGCTCATC ACAGAGACTTTCACAAGCAAATCTGCCATTTCTAAGACTGCTGAAAGCAGTGGTGGGCCCTCCACTTCCC GAGTGACAGATCCACAGGGTAAATCTGACATCCCCATGGACCTGTTTGACTTCTATGAGCAAATGGACAA GGATGAAGAAGAAGAAGAAGAGACACAGACAGTGTCTTTTGAAGTCAAGCAGGAAATGATTGAGGAACTC CAGAAACGTTGCATCCACCTGGAGTACCCTCTGTTGGCAGAATATGACTTCCGGAATGATTCTGTCAACC CTGATATCAACATTGACCTAAAGCCCACAGCTGTCCTCAGACCCTATCAGGAGAAGAGCTTGCGAAAGAT GTTTGGAAACGGGCGTGCACGTTCGGGGGTCATTGTTCTTCCCTGCGGTGCTGGAAAGTCCCTGGTTGGT GTGACTGCTGCATGCACTGTCAGAAAACGCTGTCTGGTGCTGGGCAACTCAGCTGTTTCTGTGGAGCAGT GGAAAGCCCAGTTCAAGATGTGGTCCACCATTGACGACAGCCAGATCTGCCGGTTCACCTCCGATGCCAA GGACAAGCCCATCGGCTGCTCCGTTGCCATTAGCACCTACTCCATGCTGGGCCACACCACCAAAAGGTCC TGGGAGGCCGAGCGAGTCATGGAGTGGCTCAAGACCCAGGAGTGGGGCCTCATGATCCTGGATGAAGTGC ACACCATACCAGCCAAGATGTTCCGAAGGGTGCTCACCATCGTGCAGGCCCACTGTAAGCTGGGTTTGAC TGCGACCCTCGTCCGCGAAGATGACAAAATTGTGGATTTAAATTTTCTGATTGGGCCTAAGCTCTACGAA GCCAACTGGATGGAGCTGCAGAATAATGGCTACATCGCCAAAGTCCAGTGTGCTGAGGTCTGGTGCCCTA TGTCTCCTGAATTTTACCGGGAATATGTGGCAATCAAAACCAAGAAACGAATCTTGCTGTACACCATGAA CCCCAACAAATTTAGAGCTTGCCAGTTTCTGATCAAGTTTCATGAAAGGAGGAATGACAAGATTATTGTC TTTGCTGACAATGTGTTTGCCCTAAAGGAATATGCCATTCGACTGAACAAACCCTATATCTACGGACCTA CGTCTCAGGGGGAAAGGATGCAAATTCTCCAGAATTTCAAGCACAACCCCAAAATTAACACCATCTTCAT ATCCAAGGTAGGTGACACTTCGTTTGATCTGCCGGAAGCAAATGTCCTCATTCAGATCTCATCCCATGGT GGCTCCAGGCGTCAGGAAGCCCAAAGGCTAGGGCGGGTGCTTCGAGCTAAAAAAGGGATGGTTGCAGAAG AGTACAATGCCTTTTTCTACTCACTGGTATCCCAGGACACACAGGAAATGGCTTACTCAACCAAGCGGCA GAGATTCTTGGTAGATCAAGGTTATAGCTTCAAGGTGATCACGAAACTCGCTGGCATGGAGGAGGAAGAC TTGGCGTTTTCGACAAAAGAAGAGCAACAGCAGCTCTTACAGAAAGTCCTGGCAGCCACTGACCTGGATG CCGAGGAGGAGGTGGTGGCTGGGGAATTTGGCTCCAGATCCAGCCAGGCATCTCGGCGCTTTGGCACCAT GAGTTCTATGTCTGGGGCCGACGACACTGTGTACATGGAGTACCACTCATCGCGGAGCAAGGCGCCCAGC AAACATGTACACCCGCTCTTCAAGCGCTTTAGGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202425 representing NM_000122
Red=Cloning site Green=Tags(s) MGKRDRADRDKKKSRKRHYEDEEDDEEDAPGNDPQEAVPSAAGKQVDESGTKVDEYGAKDYRLQMPLKDD HTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYL RKLSKTGVPDGIMQFIKLCTVSYGKVKLVLKHNRYFVESCHPDVIQHLLQDPVIRECRLRNSEGEATELI TETFTSKSAISKTAESSGGPSTSRVTDPQGKSDIPMDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEEL QKRCIHLEYPLLAEYDFRNDSVNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVG VTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRS WEAERVMEWLKTQEWGLMILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLYE ANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIV FADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHG GSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEED LAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSSQASRRFGTMSSMSGADDTVYMEYHSSRSKAPS KHVHPLFKRFRK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000122 |
| ORF Size | 2346 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000122.2 |
| RefSeq Size | 2751 bp |
| RefSeq ORF | 2349 bp |
| Locus ID | 2071 |
| UniProt ID | P19447 |
| Domains | DEAD, helicase_C |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Nucleotide excision repair |
| Gene Summary | This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202425 | ERCC3 (Myc-DDK-tagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3) |
CNY 5,728.00 |
|
| RC202425L1 | Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC202425L2 | Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3), mGFP tagged |
CNY 7,700.00 |
|
| RC202425L3 | Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC202425L4 | Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3), mGFP tagged |
CNY 7,700.00 |
|
| SC120091 | ERCC3 (untagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3) |
CNY 2,950.00 |
|
| SC320745 | ERCC3 (untagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3) |
CNY 2,950.00 |
