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PRP18 homolog (PRPF18) (NM_003675) Human Tagged ORF Clone
CAT＃: RG201556
- TrueORF®
PRPF18 (tGFP-tagged) - Human PRP18 pre-mRNA processing factor 18 homolog (S. cerevisiae) (PRPF18)
ORF Plasmid: DDK
"NM_003675" in other vectors (5)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | hPrp18; PRP18 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG201556 representing NM_003675
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACATTCTGAAATCAGAGATCCTTCGGAAGCGGCAGCTGGTGGAGGACAGGAACCTGCTGGTGGAAA ATAAAAAATATTTCAAGCGTAGTGAGCTCGCCAAAAAAGAAGAGGAAGCATATTTTGAAAGATGTGGCTA CAAGATACAGCCAAAAGAGGAGGACCAGAAACCATTAACTTCATCGAATCCAGTGTTAGAACTTGAACTG GCAGAGGAAAAATTACCTATGACGCTTTCTAGGCAAGAGGTCATCAGAAGATTGAGAGAAAGAGGAGAAC CAATCAGACTATTTGGAGAGACTGATTATGATGCTTTTCAACGTTTAAGGAAAATAGAGATCCTCACACC AGAAGTTAACAAGGGATTGAGGAATGATTTGAAAGCAGCCTTGGATAAGATTGATCAGCAGTACCTCAAT GAAATCGTCGGCGGTCAGGAGCCTGGAGAGGAAGACACACAGAATGATCTGAAAGTTCATGAGGAAAACA CCACAATTGAAGAGTTAGAGGCGCTTGGAGAGTCCTTAGGGAAAGGCGATGATCATAAGGACATGGACAT CATCACCAAATTCCTGAAGTTTCTTCTTGGCGTTTGGGCTAAAGAATTGAATGCCAGAGAAGATTATGTG AAACGCAGTGTGCAGGGTAAACTGAACAGTGCGACCCAGAAACAGACCGAGTCCTACCTAAGACCACTTT TTAGAAAGCTACGGAAAAGGAATCTTCCTGCTGATATTAAAGAATCAATAACGGATATTATTAAATTCAT GTTGCAGAGAGAATACGTGAAGGCAAATGATGCTTATCTTCAGATGGCCATTGGAAATGCGCCTTGGCCC ATCGGTGTCACTATGGTTGGTATCCATGCCAGAACTGGCAGAGAAAAGATTTTTTCCAAGCATGTTGCAC ATGTTTTAAATGACGAAACTCAGCGGAAATATATTCAGGGATTGAAGAGGTTAATGACCATTTGCCAGAA ACACTTTCCTACAGACCCATCCAAATGTGTGGAGTACAATGCACTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201556 representing NM_003675
Red=Cloning site Green=Tags(s) MDILKSEILRKRQLVEDRNLLVENKKYFKRSELAKKEEEAYFERCGYKIQPKEEDQKPLTSSNPVLELEL AEEKLPMTLSRQEVIRRLRERGEPIRLFGETDYDAFQRLRKIEILTPEVNKGLRNDLKAALDKIDQQYLN EIVGGQEPGEEDTQNDLKVHEENTTIEELEALGESLGKGDDHKDMDIITKFLKFLLGVWAKELNAREDYV KRSVQGKLNSATQKQTESYLRPLFRKLRKRNLPADIKESITDIIKFMLQREYVKANDAYLQMAIGNAPWP IGVTMVGIHARTGREKIFSKHVAHVLNDETQRKYIQGLKRLMTICQKHFPTDPSKCVEYNAL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003675 |
| ORF Size | 1026 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003675.3, NP_003666.1 |
| RefSeq Size | 1711 bp |
| RefSeq ORF | 1029 bp |
| Locus ID | 8559 |
| UniProt ID | Q99633 |
| Domains | SFM, Prp18 |
| Protein Pathways | Spliceosome |
| Gene Summary | Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201556 | PRPF18 (Myc-DDK-tagged)-Human PRP18 pre-mRNA processing factor 18 homolog (S. cerevisiae) (PRPF18) |
CNY 3,656.00 |
|
| RC201556L3 | Lenti ORF clone of Human PRP18 pre-mRNA processing factor 18 homolog (S. cerevisiae) (PRPF18), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201556L4 | Lenti ORF clone of Human PRP18 pre-mRNA processing factor 18 homolog (S. cerevisiae) (PRPF18), mGFP tagged |
CNY 5,890.00 |
|
| SC112701 | PRPF18 (untagged)-Human PRP18 pre-mRNA processing factor 18 homolog (S. cerevisiae) (PRPF18) |
CNY 2,950.00 |
|
| SC322203 | PRPF18 (untagged)-Human PRP18 pre-mRNA processing factor 18 homolog (S. cerevisiae) (PRPF18) |
CNY 3,656.00 |
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