ROS1 (NM_002944) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	DDK-His
Synonyms	c-ros-1; MCF3; ROS
Vector	pCMV6-XL5-DDK-His
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	ORF Nucleotide Sequence (showhide) >RC600071 representing leader sequence plus the extracellular domain region of NM_002944 Red=Cloning site Blue=ORF Green=Tags(s) GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG CGATCGCC ATGAAGAACATTTACTGTCTTATTCCGAAGCTTGTCAATTTTGCAACTCTTGGCTGCCTATGGATTTCTG TGGTGCAGTGTACAGTTTTAAATAGCTGCCTAAAGTCGTGTGTAACTAATCTGGGCCAGCAGCTTGACCT TGGCACACCACATAATCTGAGTGAACCGTGTATCCAAGGATGTCACTTTTGGAACTCTGTAGATCAGAAA AACTGTGCTTTAAAGTGTCGGGAGTCGTGTGAGGTTGGCTGTAGCAGCGCGGAAGGTGCATATGAAGAGG AAGTACTGGAAAATGCAGACCTACCAACTGCTCCCTTTGCTTCTTCCATTGGAAGCCACAATATGACATT ACGATGGAAATCTGCAAACTTCTCTGGAGTAAAATACATCATTCAGTGGAAATATGCACAACTTCTGGGA AGCTGGACTTATACTAAGACTGTGTCCAGACCGTCCTATGTGGTCAAGCCCCTGCACCCCTTCACTGAGT ACATTTTCCGAGTGGTTTGGATCTTCACAGCGCAGCTGCAGCTCTACTCCCCTCCAAGTCCCAGTTACAG GACTCATCCTCATGGAGTTCCTGAAACTGCACCTTTGATTAGGAATATTGAGAGCTCAAGTCCCGACACT GTGGAAGTCAGCTGGGATCCACCTCAATTCCCAGGTGGACCTATTTTGGGTTATAACTTAAGGCTGATCA GCAAAAATCAAAAATTAGATGCAGGGACACAGAGAACCAGTTTCCAGTTTTACTCCACTTTACCAAATAC TATCTACAGGTTTTCTATTGCAGCAGTAAATGAAGTTGGTGAGGGTCCAGAAGCAGAATCTAGTATTACC ACTTCATCTTCAGCAGTTCAACAAGAGGAACAGTGGCTCTTTTTATCCAGAAAAACTTCTCTAAGAAAGA GATCTTTAAAACATTTAGTAGATGAAGCACATTGCCTTCGGTTGGATGCTATATACCATAATATTACAGG AATATCTGTTGATGTCCACCAGCAAATTGTTTATTTCTCTGAAGGAACTCTCATATGGGCGAAGAAGGCT GCCAACATGTCTGATGTATCTGACCTGAGAATTTTTTACAGAGGTTCAGGATTAATTTCTTCTATCTCCA TAGATTGGCTTTATCAAAGAATGTATTTCATCATGGATGAACTGGTATGTGTCTGTGATTTAGAGAACTG CTCAAACATCGAGGAAATTACTCCACCCTCTATTAGTGCACCTCAAAAAATTGTGGCTGATTCATACAAT GGGTATGTCTTTTACCTCCTGAGAGATGGCATTTATAGAGCAGACCTTCCTGTACCATCTGGCCGGTGTG CAGAAGCTGTGCGTATTGTGGAGAGTTGCACGTTAAAGGACTTTGCAATCAAGCCACAAGCCAAGCGAAT CATTTACTTCAATGACACTGCCCAAGTCTTCATGTCAACATTTCTGGATGGCTCTGCTTCCCATCTCATC CTACCTCGCATCCCCTTTGCTGATGTGAAAAGTTTTGCTTGTGAAAACAATGACTTTCTTGTCACAGATG GCAAGGTCATTTTCCAACAGGATGCTTTGTCTTTTAATGAATTCATCGTGGGATGTGACCTGAGTCACAT AGAAGAATTTGGGTTTGGTAACTTGGTCATCTTTGGCTCATCCTCCCAGCTGCACCCTCTGCCAGGCCGC CCGCAGGAGCTTTCGGTGCTGTTTGGCTCTCACCAGGCTCTTGTTCAATGGAAGCCTCCTGCCCTTGCCA TAGGAGCCAATGTCATCCTGATCAGTGATATTATTGAACTCTTTGAATTAGGCCCTTCTGCCTGGCAGAA CTGGACCTATGAGGTGAAAGTATCCACCCAAGACCCTCCTGAAGTCACTCATATTTTCTTGAACATAAGT GGAACCATGCTGAATGTACCTGAGCTGCAGAGTGCTATGAAATACAAGGTTTCTGTGAGAGCAAGTTCTC CAAAGAGGCCAGGCCCCTGGTCAGAGCCCTCAGTGGGTACTACCCTGGTGCCAGCTAGTGAACCACCATT TATCATGGCTGTGAAAGAAGATGGGCTTTGGAGTAAACCATTAAATAGCTTTGGCCCAGGAGAGTTCTTA TCCTCTGATATAGGAAATGTGTCAGACATGGATTGGTATAACAACAGCCTCTACTACAGTGACACGAAAG GCGACGTTTTTGTGTGGCTGCTGAATGGGACGGATATCTCAGAGAATTATCACCTACCCAGCATTGCAGG AGCAGGGGCTTTAGCTTTTGAGTGGCTGGGTCACTTTCTCTACTGGGCTGGAAAGACATATGTGATACAA AGGCAGTCTGTGTTGACGGGACACACAGACATTGTTACCCACGTGAAGCTATTGGTGAATGACATGGTGG TGGATTCAGTTGGTGGATATCTCTACTGGACCACACTCTATTCAGTGGAAAGCACCAGACTAAATGGGGA AAGTTCCCTTGTACTACAGACACAGCCTTGGTTTTCTGGGAAAAAGGTAATTGCTCTAACTTTAGACCTC AGTGATGGGCTCCTGTATTGGTTGGTTCAAGACAGTCAATGTATTCACCTGTACACAGCTGTTCTTCGGG GACAGAGCACTGGGGATACCACCATCACAGAATTTGCAGCCTGGAGTACTTCTGAAATTTCCCAGAATGC ACTGATGTACTATAGTGGTCGGCTGTTCTGGATCAATGGCTTTAGGATTATCACAACTCAAGAAATAGGT CAGAAAACCAGTGTCTCTGTTTTGGAACCAGCCAGATTTAATCAGTTCACAATTATTCAGACATCCCTTA AGCCCCTGCCAGGGAACTTTTCCTTTACCCCTAAGGTTATTCCAGATTCTGTTCAAGAGTCTTCATTTAG GATTGAAGGAAATGCTTCAAGTTTTCAAATCCTGTGGAATGGTCCCCCTGCGGTAGACTGGGGTGTAGTT TTCTACAGTGTAGAATTTAGTGCTCATTCTAAGTTCTTGGCTAGTGAACAACACTCTTTACCTGTATTTA CTGTGGAAGGACTGGAACCTTATGCCTTATTTAATCTTTCTGTCACTCCTTATACCTACTGGGGAAAGGG CCCCAAAACATCTCTGTCACTTCGAGCACCTGAAACAGTTCCATCAGCACCAGAGAACCCCAGAATATTT ATATTACCAAGTGGAAAATGCTGCAACAAGAATGAAGTTGTGGTGGAATTTAGGTGGAACAAACCTAAGC ATGAAAATGGGGTGTTAACAAAATTTGAAATTTTCTACAATATATCCAATCAAAGTATTACAAACAAAAC ATGTGAAGACTGGATTGCTGTCAATGTCACTCCCTCAGTGATGTCTTTTCAACTTGAAGGCATGAGTCCC AGATGCTTTATTGCCTTCCAGGTTAGGGCCTTTACATCTAAGGGGCCAGGACCATATGCTGACGTTGTAA AGTCTACAACATCAGAAATCAACCCATTTCCTCACCTCATAACTCTTCTTGGTAACAAGATAGTTTTTTT AGATATGGATCAAAATCAAGTTGTGTGGACGTTTTCAGCAGAAAGAGTTATCAGTGCCGTTTGCTACACA GCTGATAATGAGATGGGATATTATGCTGAAGGGGACTCACTCTTTCTTCTGCACTTGCACAATCGCTCTA GCTCTGAGCTTTTCCAAGATTCACTGGTTTTTGATATCACAGTTATTACAATTGACTGGATTTCAAGGCA CCTCTACTTTGCACTGAAAGAATCACAAAATGGAATGCAAGTATTTGATGTTGATCTTGAACACAAGGTG AAATATCCCAGAGAGGTGAAGATTCACAATAGGAATTCAACAATAATTTCTTTTTCTGTATATCCTCTTT TAAGTCGCTTGTATTGGACAGAAGTTTCCAATTTTGGCTACCAGATGTTCTACTACAGTATTATCAGTCA CACCTTGCACCGAATTCTGCAACCCACAGCTACAAACCAACAAAACAAAAGGAATCAATGTTCTTGTAAT GTGACTGAATTTGAGTTAAGTGGAGCAATGGCTATTGATACCTCTAACCTAGAGAAACCATTGATATACT TTGCCAAAGCACAAGAGATCTGGGCAATGGATCTGGAAGGCTGTCAGTGTTGGAGAGTTATCACAGTACC TGCTATGCTCGCAGGAAAAACCCTTGTTAGCTTAACTGTGGATGGAGATCTTATATACTGGATCATCACA GCAAAGGACAGCACACAGATTTATCAGGCAAAGAAAGGAAATGGGGCCATCGTTTCCCAGGTGAAGGCCC TAAGGAGTAGGCATATCTTGGCTTACAGTTCAGTTATGCAGCCTTTTCCAGATAAAGCGTTTCTGTCTCT AGCTTCAGACACTGTGGAACCAACTATACTTAATGCCACTAACACTAGCCTCACAATCAGATTACCTCTG GCCAAGACAAACCTCACATGGTATGGCATCACCAGCCCTACTCCAACATACCTGGTTTATTATGCAGAAG TTAATGACAGGAAAAACAGCTCTGACTTGAAATATAGAATTCTGGAATTTCAGGACAGTATAGCTCTTAT TGAAGATTTACAACCATTTTCAACATACATGATACAGATAGCTGTAAAAAATTATTATTCAGATCCTTTG GAACATTTACCACCAGGAAAAGAGATTTGGGGAAAAACTAAAAATGGAGTACCAGAGGCAGTGCAGCTCA TTAATACAACTGTGCGGTCAGACACCAGCCTCATTATATCTTGGAGAGAATCTCACAAGCCAAATGGACC TAAAGAATCAGTCCGTTATCAGTTGGCAATCTCACACCTGGCCCTAATTCCTGAAACTCCTCTAAGACAA AGTGAATTTCCAAATGGAAGGCTCACTCTCCTTGTTACTAGACTGTCTGGTGGAAATATTTATGTGTTAA AGGTTCTTGCCTGCCACTCTGAGGAAATGTGGTGTACAGAGAGTCATCCTGTCACTGTGGAAATGTTTAA CACACCAGAGAAACCTTATTCCTTGGTTCCAGAGAACACTAGTTTGCAATTTAATTGGAAGGCTCCATTG AATGTTAACCTCATCAGATTTTGGGTTGAGCTACAGAAGTGGAAATACAATGAGTTTTACCATGTTAAAA CTTCATGCAGCCAAGGTCCTGCTTATGTCTGTAATATCACAAATCTACAACCTTATACTTCATATAATGT CAGAGTAGTGGTGGTTTATAAGACGGGAGAAAATAGCACCTCACTTCCAGAAAGCTTTAAGACAAAAGCT GGAGTCCCAAATAAACCAGGCATTCCCAAATTACTAGAAGGGAGTAAAAATTCAATACAGTGGGAGAAAG CTGAAGATAATGGATGTAGAATTACATACTATATCCTTGAGATAAGAAAGAGCACTTCAAATAATTTACA GAACCAGAATTTAAGGTGGAAGATGACATTTAATGGATCCTGCAGTAGTGTTTGCACATGGAAGTCCAAA AACCTGAAAGGAATATTTCAGTTCAGAGTAGTAGCTGCAAATAATCTAGGGTTTGGTGAATATAGTGGAA TCAGTGAGAATATTATATTAGTTGGAGATGATTTTTGGATACCAGAA ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC TGGTACCGATATCAAGCTTGTCGACTCTAGA Protein Sequence (showhide) >RC600071 representing signal peptide plus the extracellular domain region of NM_002944 Red=Cloning sites Green= DDK and 6XHIS Tags MKNIYCLIPKLVNFATLGCLWISVVQCTVLNSCLKSCVTNLGQQLDLGTPHNLSEPCIQGCHFWNSVDQK NCALKCRESCEVGCSSAEGAYEEEVLENADLPTAPFASSIGSHNMTLRWKSANFSGVKYIIQWKYAQLLG SWTYTKTVSRPSYVVKPLHPFTEYIFRVVWIFTAQLQLYSPPSPSYRTHPHGVPETAPLIRNIESSSPDT VEVSWDPPQFPGGPILGYNLRLISKNQKLDAGTQRTSFQFYSTLPNTIYRFSIAAVNEVGEGPEAESSIT TSSSAVQQEEQWLFLSRKTSLRKRSLKHLVDEAHCLRLDAIYHNITGISVDVHQQIVYFSEGTLIWAKKA ANMSDVSDLRIFYRGSGLISSISIDWLYQRMYFIMDELVCVCDLENCSNIEEITPPSISAPQKIVADSYN GYVFYLLRDGIYRADLPVPSGRCAEAVRIVESCTLKDFAIKPQAKRIIYFNDTAQVFMSTFLDGSASHLI LPRIPFADVKSFACENNDFLVTDGKVIFQQDALSFNEFIVGCDLSHIEEFGFGNLVIFGSSSQLHPLPGR PQELSVLFGSHQALVQWKPPALAIGANVILISDIIELFELGPSAWQNWTYEVKVSTQDPPEVTHIFLNIS GTMLNVPELQSAMKYKVSVRASSPKRPGPWSEPSVGTTLVPASEPPFIMAVKEDGLWSKPLNSFGPGEFL SSDIGNVSDMDWYNNSLYYSDTKGDVFVWLLNGTDISENYHLPSIAGAGALAFEWLGHFLYWAGKTYVIQ RQSVLTGHTDIVTHVKLLVNDMVVDSVGGYLYWTTLYSVESTRLNGESSLVLQTQPWFSGKKVIALTLDL SDGLLYWLVQDSQCIHLYTAVLRGQSTGDTTITEFAAWSTSEISQNALMYYSGRLFWINGFRIITTQEIG QKTSVSVLEPARFNQFTIIQTSLKPLPGNFSFTPKVIPDSVQESSFRIEGNASSFQILWNGPPAVDWGVV FYSVEFSAHSKFLASEQHSLPVFTVEGLEPYALFNLSVTPYTYWGKGPKTSLSLRAPETVPSAPENPRIF ILPSGKCCNKNEVVVEFRWNKPKHENGVLTKFEIFYNISNQSITNKTCEDWIAVNVTPSVMSFQLEGMSP RCFIAFQVRAFTSKGPGPYADVVKSTTSEINPFPHLITLLGNKIVFLDMDQNQVVWTFSAERVISAVCYT ADNEMGYYAEGDSLFLLHLHNRSSSELFQDSLVFDITVITIDWISRHLYFALKESQNGMQVFDVDLEHKV KYPREVKIHNRNSTIISFSVYPLLSRLYWTEVSNFGYQMFYYSIISHTLHRILQPTATNQQNKRNQCSCN VTEFELSGAMAIDTSNLEKPLIYFAKAQEIWAMDLEGCQCWRVITVPAMLAGKTLVSLTVDGDLIYWIIT AKDSTQIYQAKKGNGAIVSQVKALRSRHILAYSSVMQPFPDKAFLSLASDTVEPTILNATNTSLTIRLPL AKTNLTWYGITSPTPTYLVYYAEVNDRKNSSDLKYRILEFQDSIALIEDLQPFSTYMIQIAVKNYYSDPL EHLPPGKEIWGKTKNGVPEAVQLINTTVRSDTSLIISWRESHKPNGPKESVRYQLAISHLALIPETPLRQ SEFPNGRLTLLVTRLSGGNIYVLKVLACHSEEMWCTESHPVTVEMFNTPEKPYSLVPENTSLQFNWKAPL NVNLIRFWVELQKWKYNEFYHVKTSCSQGPAYVCNITNLQPYTSYNVRVVVVYKTGENSTSLPESFKTKA GVPNKPGIPKLLEGSKNSIQWEKAEDNGCRITYYILEIRKSTSNNLQNQNLRWKMTFNGSCSSVCTWKSK NLKGIFQFRVVAANNLGFGEYSGISENIILVGDDFWIPE TRSGTRSGDYKDDDDKGSHHHHHH
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NM_002944
ORF Size	5577 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_002944.2, NP_002935.2
RefSeq Size	7368 bp
RefSeq ORF	7044 bp
Locus ID	6098
UniProt ID	P08922
Protein Families	Druggable Genome, Protein Kinase, Transmembrane
MW	209.2 kDa
Gene Summary	This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq, Jul 2008]

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