ROR1 (NM_005012) Human Tagged ORF Clone
CAT#: RC600058
- TrueORF®
ROR1 (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens receptor tyrosine kinase-like orphan receptor 1, transcript variant 1, Signal peptide (1-29) plus EC domain (30-406)
"NM_005012" in other vectors (7)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 4,560.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | DDK-His |
Synonyms | dJ537F10.1; NTRKR1 |
Vector | pCMV6-XL5-DDK-His |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>RC600058 representing leader sequence plus the extracellular domain region of NM_005012
Red=Cloning site Blue=ORF Green=Tags(s) GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG CGATCGCC ATGCACCGGCCGCGCCGCCGCGGGACGCGCCCGCCGCTCCTGGCGCTGCTGGCCGCGCTGCTGCTGGCCG CACGCGGGGCTGCTGCCCAAGAAACAGAGCTGTCAGTCAGTGCTGAATTAGTGCCTACCTCATCATGGAA CATCTCAAGTGAACTCAACAAAGATTCTTACCTGACCCTCGATGAACCAATGAATAACATCACCACGTCT CTGGGCCAGACAGCAGAACTGCACTGCAAAGTCTCTGGGAATCCACCTCCCACCATCCGCTGGTTCAAAA ATGATGCTCCTGTGGTCCAGGAGCCCCGGAGGCTCTCCTTTCGGTCCACCATCTATGGCTCTCGGCTGCG GATTAGAAACCTCGACACCACAGACACAGGCTACTTCCAGTGCGTGGCAACAAACGGCAAGGAGGTGGTT TCTTCCACTGGAGTCTTGTTTGTCAAGTTTGGCCCCCCTCCCACTGCAAGTCCAGGATACTCAGATGAGT ATGAAGAAGATGGATTCTGTCAGCCATACAGAGGGATTGCATGTGCAAGATTTATTGGCAACCGCACCGT CTATATGGAGTCTTTGCACATGCAAGGGGAAATAGAAAATCAGATCACAGCTGCCTTCACTATGATTGGC ACTTCCAGTCACTTATCTGATAAGTGTTCTCAGTTCGCCATTCCTTCCCTGTGCCACTATGCCTTCCCGT ACTGCGATGAAACTTCATCCGTCCCAAAGCCCCGTGACTTGTGTCGCGATGAATGTGAAATCCTGGAGAA TGTCCTGTGTCAAACAGAGTACATTTTTGCAAGATCAAATCCCATGATTCTGATGAGGCTGAAACTGCCA AACTGTGAAGATCTCCCCCAGCCAGAGAGCCCAGAAGCTGCGAACTGTATCCGGATTGGAATTCCCATGG CAGATCCTATAAATAAAAATCACAAGTGTTATAACAGCACAGGTGTGGACTACCGGGGGACCGTCAGTGT GACCAAATCAGGGCGCCAGTGCCAGCCATGGAATTCCCAGTATCCCCACACACACACTTTCACCGCCCTT CGTTTCCCAGAGCTGAATGGAGGCCATTCCTACTGCCGCAACCCAGGGAATCAAAAGGAAGCTCCCTGGT GCTTCACCTTGGATGAAAACTTTAAGTCTGATCTGTGTGACATCCCAGCGTGCGATTCAAAGGATTCCAA GGAGAAGAATAAAATGGAAATCCTGTAC ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC TGGTACCGATATCAAGCTTGTCGACTCTAGA >RC600058 representing signal peptide plus the extracellular domain region of NM_005012
Red=Cloning sites Green= DDK and 6XHIS Tags MHRPRRRGTRPPLLALLAALLLAARGAAAQETELSVSAELVPTSSWNISSELNKDSYLTLDEPMNNITTS LGQTAELHCKVSGNPPPTIRWFKNDAPVVQEPRRLSFRSTIYGSRLRIRNLDTTDTGYFQCVATNGKEVV SSTGVLFVKFGPPPTASPGYSDEYEEDGFCQPYRGIACARFIGNRTVYMESLHMQGEIENQITAAFTMIG TSSHLSDKCSQFAIPSLCHYAFPYCDETSSVPKPRDLCRDECEILENVLCQTEYIFARSNPMILMRLKLP NCEDLPQPESPEAANCIRIGIPMADPINKNHKCYNSTGVDYRGTVSVTKSGRQCQPWNSQYPHTHTFTAL RFPELNGGHSYCRNPGNQKEAPWCFTLDENFKSDLCDIPACDSKDSKEKNKMEILY TRSGTRSGDYKDDDDKGSHHHHHH |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_005012 |
ORF Size | 1218 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005012.3, NP_005003.2 |
RefSeq Size | 5871 bp |
RefSeq ORF | 2814 bp |
Locus ID | 4919 |
UniProt ID | Q01973 |
Domains | KR, FRI, pkinase, TyrKc, S_TKc, ig, IGc2, IG |
Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
MW | 45.5 kDa |
Gene Summary | This gene encodes a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with B-cell chronic lymphocytic leukaemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214967 | ROR1 (Myc-DDK-tagged)-Human receptor tyrosine kinase-like orphan receptor 1 (ROR1), transcript variant 1 |
CNY 10,296.00 |
|
RC214967L1 | Lenti ORF clone of Human receptor tyrosine kinase-like orphan receptor 1 (ROR1), transcript variant 1, Myc-DDK-tagged |
CNY 12,696.00 |
|
RC214967L2 | Lenti ORF clone of Human receptor tyrosine kinase-like orphan receptor 1 (ROR1), transcript variant 1, mGFP tagged |
CNY 12,696.00 |
|
RC214967L3 | Lenti ORF clone of Human receptor tyrosine kinase-like orphan receptor 1 (ROR1), transcript variant 1, Myc-DDK-tagged |
CNY 12,696.00 |
|
RC214967L4 | Lenti ORF clone of Human receptor tyrosine kinase-like orphan receptor 1 (ROR1), transcript variant 1, mGFP tagged |
CNY 12,696.00 |
|
RG214967 | ROR1 (tGFP-tagged) - Human receptor tyrosine kinase-like orphan receptor 1 (ROR1), transcript variant 1 |
CNY 11,896.00 |
|
SC117005 | ROR1 (untagged)-Human receptor tyrosine kinase-like orphan receptor 1 (ROR1), transcript variant 1 |
CNY 10,304.00 |