TRIP12 (NM_001284214) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MRD49; TRIP-12; TRIPC; ULF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240228 representing NM_001284214 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAACCGGCCTAATAACAATCCAGGGGGGTCACTGCGACGTTCACAGAGGAACACTGCCGGGGCCC AACCACAAGACGACTCAATAGGAGGAAGGTCACATTTAGGGCAGGCAAAACATAAGGGATATAGCCCTCC TGAGAGTAGAAAATCTAATTCTAAGGCACCCAAAGTGCAGTCTAATACTACTTCTGAACTGTCAAGGGGA CACCTTTCTAAAAGAAGCTGCAGTTCATCATCTGCTGTGATAGTTCCACAACCAGAGGATCCAGACAGAG CCAATACTTCAGAAAGACAAAAAACGGGGCAGGTGCCTAAGAAAGACAATTCTCGAGGAGTGAAGCGCAG TGCTAGTCCAGACTACAACAGGACCAATTCTCCTAGCTCTGCAAAAAAACCAAAAGCACTTCAGCATACT GAATCTCCCTCAGAAACAAATAAGCCACATAGTAAGTCAAAGAAGAGACATTTAGACCAGGAGCAACAAC TGAAATCTGCACAATCACCATCAACAAGCAAGGCTCATACCAGGAAGAGTGGGGCCACTGGCGGTTCACG GAGTCAGAAAAGAAAAAGGACAGAGAGTTCTTGTGTAAAGAGTGGCTCCGGGTCTGAATCAACTGGTGCA GAAGAGAGATCTGCGAAACCTACCAAGCTGGCTTCAAAATCAGCCACCTCAGCCAAAGCTGGGTGTAGCA CCATCACTGATTCTTCTTCTGCTGCCTCTACTTCCTCCTCGTCTTCTGCTGTAGCCTCGGCCTCCTCCAC TGTACCACCAGGTGCCAGAGTGAAACAAGGAAAAGATCAGAACAAGGCCAGGCGTTCCCGTTCAGCGTCC AGTCCCAGCCCCAGAAGAAGTAGCAGGGAAAAGGAACAGAGTAAAACTGGTGGCTCTTCAAAATTTGATT GGGCTGCTCGTTTCAGCCCTAAAGTTAGCCTTCCTAAAACAAAACTGTCTCTTCCAGGGTCTTCTAAGTC AGAGACATCAAAACCTGGACCTTCTGGATTACAGGCCAAATTAGCAAGTTTAAGAAAATCTACGAAGAAA CGCAGTGAGTCTCCACCTGCTGAGCTCCCCAGTTTGAGGCGGAGCACACGCCAAAAGACCACGGGCTCCT GTGCTAGTACCAGTCGGCGAGGCTCTGGCCTGGGCAAAAGAGGAGCAGCTGAAGCTCGTCGACAGGAGAA AATGGCAGACCCTGAAAGCAACCAGGAGGCAGTAAATTCTTCAGCTGCTCGGACAGATGAAGCTCCCCAA GGAGCTGCAGCCTCTAGTTCTGTTGCAGGGGCTGTTGGCATGACCACCTCTGGGGAGAGTGAATCAGATG ATTCCGAGATGGGACGTTTGCAAGCTTTGTTAGAGGCAAGGGGTCTTCCCCCTCACCTATTTGGTCCTCT TGGTCCTCGGATGTCACAGCTTTTCCATAGAACAATTGGAAGTGGAGCTAGTTCTAAGGCCCAGCAGCTA CTACAAGGATTGCAAGCCAGTGATGAAAGTCAACAGCTTCAGGCAGTTATTGAGATGTGTCAGTTACTGG TCATGGGAAATGAGGAGACACTGGGAGGGTTTCCTGTCAAGAGTGTTGTTCCAGCTTTGATTACGTTACT TCAGATGGAGCACAATTTTGATATTATGAACCATGCTTGTCGAGCCTTAACATACATGATGGAAGCACTT CCTCGATCTTCTGCTGTTGTAGTAGATGCTATTCCTGTCTTTTTAGAAAAGCTGCAAGTTATTCAGTGTA TTGATGTGGCAGAGCAGGCCTTGACTGCCTTGGAGATGTTGTCACGGAGACATAGTAAAGCCATTCTACA GGCGGGTGGTTTGGCAGACTGCTTGCTGTACCTAGAATTCTTCAGCATAAATGCCCAAAGAAATGCATTA GCAATTGCAGCTAATTGCTGCCAGAGTATCACGCCAGATGAATTTCATTTTGTGGCAGATTCACTCCCAT TGCTAACCCAAAGGCTAACACATCAGGATAAAAAGTCAGTAGAAAGCACTTGCCTTTGTTTTGCACGCCT AGTGGACAACTTCCAGCATGAGGAGAATTTACTCCAGCAGGTTGCTTCCAAAGATCTGCTTACAAATGTT CAACAGCTGTTGGTAGTGACTCCACCCATTTTAAGTTCTGGGATGTTTATAATGGTGGTTCGCATGTTTT CTCTGATGTGTTCCAACTGTCCAACTTTAGCTGTTCAACTTATGAAACAAAACATTGCAGAAACGCTTCA CTTTCTCCTGTGTGGTGCCTCCAATGGAAGTTGTCAGGAACAGATTGATCTTGTTCCACGAAGCCCTCAA GAGTTGTATGAACTGACATCTCTGATTTGTGAACTTATGCCATGTTTACCAAAAGAAGGCATTTTTGCAG TTGATACCATGTTGAAGAAGGGAAATGCACAGAACACAGATGGTGCGATATGGCAGTGGCGTGATGATCG GGGCCTCTGGCATCCATATAACAGGATTGACAGCCGGATCATTGAGCAAATCAATGAGGACACGGGAACA GCACGTGCCATTCAGAGAAAACCTAACCCGTTAGCCAATAGTAACACTAGTGGATATTCAGAGTCAAAGA AGGATGATGCTCGAGCACAGCTTATGAAAGAGGATCCGGAACTGGCTAAGTCTTTTATTAAGACATTATT TGGTGTTCTTTATGAAGTGTATAGTTCCTCAGCAGGACCTGCGGTCAGACATAAGTGCCTTAGAGCAATT CTTAGGATAATTTATTTTGCGGATGCTGAACTTCTGAAGGATGTTCTGAAAAATCATGCTGTTTCAAGTC ACATTGCTTCCATGCTGTCAAGCCAAGACCTGAAGATAGTAGTGGGAGCACTTCAGATGGCAGAAATTTT AATGCAGAAGTTACCTGATATTTTTAGTGTTTACTTCAGAAGAGAAGGTGTAATGCATCAAGTAAAACAC TTAGCAGAATCAGAGTCTTTGTTGACAAGTCCACCAAAGGCATGTACGAATGGATCGGGATCCATGGGAT CCACAACTTCAGTCAGCAGTGGGACAGCCACAGCTGCCACTCATGCTGCAGCTGACTTGGGATCACCCAG CTTGCAGCACAGCAGGGATGATTCTTTAGATCTCAGCCCTCAAGGTCGATTAAGTGATGTTCTAAAGAGA AAACGACTGCCAAAACGAGGGCCAAGAAGGCCAAAGTACTCACCTCCAAGAGATGATGACAAAGTAGACA ATCAAGCTAAAAGCCCCACCACTACTCAGTCACCTAAATCTTCTTTCCTGGCAAGCTTGAATCCAAAAAC ATGGGGAAGGTTAAGTACACAGTCCAACAGCAACAACATTGAGCCAGCACGGACTGCGGGAGGTAGTGGC CTTGCCAGGGCTGCCTCAAAGGATACCATCTCCAATAATAGAGAAAAAATTAAAGGTTGGATTAAGGAGC AGGCACATAAATTTGTAGAACGTTATTTCAGTTCTGAGAATATGGATGGAAGCAACCCTGCATTGAATGT CCTTCAGAGACTTTGTGCTGCAACCGAACAACTCAACCTCCAGGTGGATGGTGGAGCTGAGTGCCTTGTA GAAATCCGTAGCATAGTCTCAGAGTCAGATGTTTCATCATTTGAAATCCAACATAGTGGATTTGTGAAGC AGCTGTTGCTTTATTTGACATCTAAAAGTGAAAAGGATGCTGTGAGCAGAGAGATCAGATTAAAGCGATT TCTTCATGTATTTTTTTCTTCTCCACTTCCTGGAGAAGAGCCCATTGGAAGAGTGGAACCAGTGGGTAAT GCACCTTTGTTGGCATTAGTTCACAAGATGAACAACTGCCTCAGCCAGATGGAACAATTTCCAGTCAAAG TACATGATTTCCCTAGTGGAAATGGGACAGGAGGCAGCTTTTCTCTCAACAGAGGATCACAGGCTTTAAA ATTTTTCAACACACATCAATTAAAATGCCAGTTACAAAGGCATCCAGACTGTGCAAATGTGAAGCAGTGG AAGGGTGGACCTGTCAAGATTGACCCTCTGGCTTTGGTACAAGCCATCGAGAGATACCTTGTAGTTAGAG GGTATGGAAGAGTAAGAGAAGATGATGAAGACAGCGATGACGATGGATCAGATGAGGAAATAGATGAGTC TCTGGCTGCTCAGTTCCTAAATTCAGGAAATGTAAGACACAGGCTGCAGTTTTATATTGGAGAACATTTG CTGCCGTATAACATGACTGTGTATCAGGCAGTACGGCAGTTTAGTATACAGGCTGAAGATGAAAGAGAAT CCACAGATGATGAGAGCAATCCTCTAGGCAGAGCTGGTATTTGGACAAAGACTCATACAATATGGTATAA ACCTGTGAGAGAGGATGAAGAAAGTAATAAAGATTGTGTTGGTGGTAAAAGAGGAAGAGCCCAAACAGCT CCAACGAAAACTTCCCCTAGAAATGCAAAAAAGCATGATGAGTTATGGCACGATGGAGTGTGCCCATCAG TATCAAATCCTTTAGAAGTTTACCTCATTCCCACACCACCTGAAAATATAACATTTGAAGACCCGTCATT AGATGTGATCCTTCTTTTAAGAGTTTTACATGCTATCAGTCGATACTGGTATTACTTGTATGATAATGCA ATGTGCAAGGAAATTATTCCAACTAGTGAATTTATTAACAGTAAGTTAACAGCAAAAGCAAATAGGCAAC TTCAAGATCCTTTAGTAATCATGACAGGAAACATCCCAACATGGCTTACTGAGCTAGGAAAAACCTGCCC ATTTTTCTTTCCTTTTGATACCCGGCAAATGCTTTTTTATGTAACTGCATTTGATCGGGACCGAGCAATG CAAAGATTACTTGATACCAACCCAGAAATCAACCAGTCTGATTCTCAAGATAGCAGAGTTGCACCTAGAT TGGATAGAAAAAAACGTACTGTGAACCGAGAGGAGCTGCTGAAACAGGCGGAGTCTGTGATGCAGGACCT CGGCAGCTCACGGGCCATGTTAGAAATCCAGTATGAAAATGAGGTTGGTACAGGTCTTGGGCCTACACTG GAGTTTTATGCGCTTGTATCTCAGGAACTACAGAGAGCTGACTTGGGTCTTTGGAGAGGTGAAGAAGTAA CTCTTAGCAATCCAAAAGGGAGCCAAGAAGGGACCAAGTATATTCAAAACCTCCAGGGCCTGTTTGCGCT TCCCTTTGGTAGGACAGCAAAGCCAGCTCATATCGCAAAGGTTAAGATGAAGTTTCGCTTCTTAGGAAAA TTAATGGCCAAGGCTATCATGGATTTCAGATTGGTGGACCTTCCCCTTGGCTTACCCTTTTATAAATGGA TGCTACGGCAAGAAACTTCACTGACATCACACGATTTGTTTGACATCGACCCAGTTGTAGCCAGATCAGT TTATCACCTAGAAGACATTGTCAGACAGAAGAAAAGACTTGAACAAGATAAATCCCAGACCAAAGAGAGT CTACAGTATGCATTAGAAACCTTGACTATGAATGGCTGCTCAGTTGAAGATCTAGGACTGGATTTCACTC TGCCAGGGTTTCCCAATATCGAACTGAAGAAAGGAGGGAAGGATATACCAGTCACTATCCACAATTTAGA GGAGTATCTAAGACTGGTTATATTCTGGGCACTAAATGAAGGCGTTTCTAGGCAATTTGATTCGTTCAGA GATGGATTTGAATCAGTCTTCCCACTCAGTCATCTTCAGTACTTCTACCCGGAGGAACTGGATCAGCTCC TTTGTGGCAGTAAAGCAGACACTTGGGATGCAAAGACACTGATGGAATGCTGTAGGCCTGATCATGGTTA TACTCATGACAGTCGGGCTGTGAAGTTTTTGTTTGAGATTCTCAGTAGTTTTGATAATGAGCAGCAGAGG TTATTTCTCCAGTTTGTGACTGGTAGCCCAAGATTGCCTGTTGGAGGATTCCGGAGTTTGAATCCACCTT TGACAATTGTCCGAAAGACGTTTGAATCAACAGAAAACCCAGATGACTTCTTGCCCTCTGTAATGACTTG TGTGAACTATCTTAAGTTGCCGGACTATTCAAGCATTGAGATAATGCGTGAAAAACTGTTGATAGCAGCA AGAGAAGGGCAGCAGTCGTTCCATCTTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240228 representing NM_001284214 Red=Cloning site Green=Tags(s) MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRG HLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHT ESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGA EERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSAS SPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKK RSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQ GAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQL LQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEAL PRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNAL AIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNV QQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQ ELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGT ARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAI LRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKH LAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKR KRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSG LARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLV EIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGN APLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQW KGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHL LPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTA PTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNA MCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAM QRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTL EFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGK LMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKES LQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFR DGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQR LFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAA REGQQSFHLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284214
ORF Size	6120 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284214.2
RefSeq Size	10036 bp
RefSeq ORF	6123 bp
Locus ID	9320
UniProt ID	Q14669
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
MW	226 kDa
Gene Summary	The protein encoded by this gene is an E3 ubiquitin-protein ligase involved in the degradation of the p19ARF/ARF isoform of CDKN2A, a tumor suppressor. The encoded protein also plays a role in the DNA damage response by regulating the stability of USP7, which regulates tumor suppressor p53. [provided by RefSeq, Jan 2017]

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