TRIP12 (NM_001284215) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MRD49; TRIP-12; TRIPC; ULF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240227 representing NM_001284215 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAACCGGCCTAATAACAATCCAGGGGGGTCACTGCGACGTTCACAGAGGAACACTGCCGGGGCCC AACCACAAGACGACTCAATAGGAGGAAGAAGCTGCAGTTCATCATCTGCTGTGATAGTTCCACAACCAGA GGATCCAGACAGAGCCAATACTTCAGAAAGACAAAAAACGGGGCAGGTGCCTAAGAAAGACAATTCTCGA GGAGTGAAGCGCAGTGCTAGTCCAGACTACAACAGGACCAATTCTCCTAGCTCTGCAAAAAAACCAAAAG CACTTCAGCATACTGAATCTCCCTCAGAAACAAATAAGCCACATAGTAAGTCAAAGAAGAGACATTTAGA CCAGGAGCAACAACTGAAATCTGCACAATCACCATCAACAAGCAAGGCTCATACCAGGAAGAGTGGGGCC ACTGGCGGTTCACGGAGTCAGAAAAGAAAAAGGACAGAGAGTTCTTGTGTAAAGAGTGGCTCCGGGTCTG AATCAACTGGTGCAGAAGAGAGATCTGCGAAACCTACCAAGCTGGCTTCAAAATCAGCCACCTCAGCCAA AGCTGGGTGTAGCACCATCACTGATTCTTCTTCTGCTGCCTCTACTTCCTCCTCGTCTTCTGCTGTAGCC TCGGCCTCCTCCACTGTACCACCAGGTGCCAGAGTGAAACAAGGAAAAGATCAGAACAAGGCCAGGCGTT CCCGTTCAGCGTCCAGTCCCAGCCCCAGAAGAAGTAGCAGGGAAAAGGAACAGAGTAAAACTGGTGGCTC TTCAAAATTTGATTGGGCTGCTCGTTTCAGCCCTAAAGTTAGCCTTCCTAAAACAAAACTGTCTCTTCCA GGGTCTTCTAAGTCAGAGACATCAAAACCTGGACCTTCTGGATTACAGGCCAAATTAGCAAGTTTAAGAA AATCTACGAAGAAACGCAGTGAGTCTCCACCTGCTGAGCTCCCCAGTTTGAGGCGGAGCACACGCCAAAA GACCACGGGCTCCTGTGCTAGTACCAGTCGGCGAGGCTCTGGCCTGGGCAAAAGAGGAGCAGCTGAAGCT CGTCGACAGGAGAAAATGGCAGACCCTGAAAGCAACCAGGAGGCAGTAAATTCTTCAGCTGCTCGGACAG ATGAAGCTCCCCAAGGAGCTGCAGCCTCTAGTTCTGTTGCAGGGGCTGTTGGCATGACCACCTCTGGGGA GAGTGAATCAGATGATTCCGAGATGGGACGTTTGCAAGCTTTGTTAGAGGCAAGGGGTCTTCCCCCTCAC CTATTTGGTCCTCTTGGTCCTCGGATGTCACAGCTTTTCCATAGAACAATTGGAAGTGGAGCTAGTTCTA AGGCCCAGCAGCTACTACAAGGATTGCAAGCCAGTGATGAAAGTCAACAGCTTCAGGCAGTTATTGAGAT GTGTCAGTTACTGGTCATGGGAAATGAGGAGACACTGGGAGGGTTTCCTGTCAAGAGTGTTGTTCCAGCT TTGATTACGTTACTTCAGATGGAGCACAATTTTGATATTATGAACCATGCTTGTCGAGCCTTAACATACA TGATGGAAGCACTTCCTCGATCTTCTGCTGTTGTAGTAGATGCTATTCCTGTCTTTTTAGAAAAGCTGCA AGTTATTCAGTGTATTGATGTGGCAGAGCAGGCCTTGACTGCCTTGGAGATGTTGTCACGGAGACATAGT AAAGCCATTCTACAGGCGGGTGGTTTGGCAGACTGCTTGCTGTACCTAGAATTCTTCAGCATAAATGCCC AAAGAAATGCATTAGCAATTGCAGCTAATTGCTGCCAGAGTATCACGCCAGATGAATTTCATTTTGTGGC AGATTCACTCCCATTGCTAACCCAAAGGCTAACACATCAGGATAAAAAGTCAGTAGAAAGCACTTGCCTT TGTTTTGCACGCCTAGTGGACAACTTCCAGCATGAGGAGAATTTACTCCAGCAGGTTGCTTCCAAAGATC TGCTTACAAATGTTCAACAGCTGTTGGTAGTGACTCCACCCATTTTAAGTTCTGGGATGTTTATAATGGT GGTTCGCATGTTTTCTCTGATGTGTTCCAACTGTCCAACTTTAGCTGTTCAACTTATGAAACAAAACATT GCAGAAACGCTTCACTTTCTCCTGTGTGGTGCCTCCAATGGAAGTTGTCAGGAACAGATTGATCTTGTTC CACGAAGCCCTCAAGAGTTGTATGAACTGACATCTCTGATTTGTGAACTTATGCCATGTTTACCAAAAGA AGGCATTTTTGCAGTTGATACCATGTTGAAGAAGGGAAATGCACAGAACACAGATGGTGCGATATGGCAG TGGCGTGATGATCGGGGCCTCTGGCATCCATATAACAGGATTGACAGCCGGATCATTGAGGCAGCCCATC AGGTCGGTGAGGATGAGATAAGCTTGTCCACTCTGGGACGAGTTTATACTATTGATTTTAATTCTATGCA GCAAATCAATGAGGACACGGGAACAGCACGTGCCATTCAGAGAAAACCTAACCCGTTAGCCAATAGTAAC ACTAGTGGATATTCAGAGTCAAAGAAGGATGATGCTCGAGCACAGCTTATGAAAGAGGATCCGGAACTGG CTAAGTCTTTTATTAAGACATTATTTGGTGTTCTTTATGAAGTGTATAGTTCCTCAGCAGGACCTGCGGT CAGACATAAGTGCCTTAGAGCAATTCTTAGGATAATTTATTTTGCGGATGCTGAACTTCTGAAGGATGTT CTGAAAAATCATGCTGTTTCAAGTCACATTGCTTCCATGCTGTCAAGCCAAGACCTGAAGATAGTAGTGG GAGCACTTCAGATGGCAGAAATTTTAATGCAGAAGTTACCTGATATTTTTAGTGTTTACTTCAGAAGAGA AGGTGTAATGCATCAAGTAAAACACTTAGCAGAATCAGAGTCTTTGTTGACAAGTCCACCAAAGGCATGT ACGAATGGATCGGGATCCATGGGATCCACAACTTCAGTCAGCAGTGGGACAGCCACAGCTGCCACTCATG CTGCAGCTGACTTGGGATCACCCAGCTTGCAGCACAGCAGGGATGATTCTTTAGATCTCAGCCCTCAAGG TCGATTAAGTGATGTTCTAAAGAGAAAACGACTGCCAAAACGAGGGCCAAGAAGGCCAAAGTACTCACCT CCAAGAGATGATGACAAAGTAGACAATCAAGCTAAAAGCCCCACCACTACTCAGTCACCTAAATCTTCTT TCCTGGCAAGCTTGAATCCAAAAACATGGGGAAGGTTAAGTACACAGTCCAACAGCAACAACATTGAGCC AGCACGGACTGCGGGAGGTAGTGGCCTTGCCAGGGCTGCCTCAAAGGATACCATCTCCAATAATAGAGAA AAAATTAAAGGTTGGATTAAGGAGCAGGCACATAAATTTGTAGAACGTTATTTCAGTTCTGAGAATATGG ATGGAAGCAACCCTGCATTGAATGTCCTTCAGAGACTTTGTGCTGCAACCGAACAACTCAACCTCCAGGT GGATGGTGGAGCTGAGTGCCTTGTAGAAATCCGTAGCATAGTCTCAGAGTCAGATGTTTCATCATTTGAA ATCCAACATAGTGGATTTGTGAAGCAGCTGTTGCTTTATTTGACATCTAAAAGTGAAAAGGATGCTGTGA GCAGAGAGATCAGATTAAAGCGATTTCTTCATGTATTTTTTTCTTCTCCACTTCCTGGAGAAGAGCCCAT TGGAAGAGTGGAACCAGTGGGTAATGCACCTTTGTTGGCATTAGTTCACAAGATGAACAACTGCCTCAGC CAGATGGAACAATTTCCAGTCAAAGTACATGATTTCCCTAGTGGAAATGGGACAGGAGGCAGCTTTTCTC TCAACAGAGGATCACAGGCTTTAAAATTTTTCAACACACATCAATTAAAATGCCAGTTACAAAGGCATCC AGACTGTGCAAATGTGAAGCAGTGGAAGGGTGGACCTGTCAAGATTGACCCTCTGGCTTTGGTACAAGCC ATCGAGAGATACCTTGTAGTTAGAGGGTATGGAAGAGTAAGAGAAGATGATGAAGACAGCGATGACGATG GATCAGATGAGGAAATAGATGAGTCTCTGGCTGCTCAGTTCCTAAATTCAGGAAATGTAAGACACAGGCT GCAGTTTTATATTGGAGAACATTTGCTGCCGTATAACATGACTGTGTATCAGGCAGTACGGCAGTTTAGT ATACAGGCTGAAGATGAAAGAGAATCCACAGATGATGAGAGCAATCCTCTAGGCAGAGCTGGTATTTGGA CAAAGACTCATACAATATGGTATAAACCTGTGAGAGAGGATGAAGAAAGTAATAAAGATTGTGTTGGTGG TAAAAGAGGAAGAGCCCAAACAGCTCCAACGAAAACTTCCCCTAGAAATGCAAAAAAGCATGATGAGTTA TGGCACGATGGAGTGTGCCCATCAGTATCAAATCCTTTAGAAGTTTACCTCATTCCCACACCACCTGAAA ATATAACATTTGAAGACCCGTCATTAGATGTGATCCTTCTTTTAAGAGTTTTACATGCTATCAGTCGATA CTGGTATTACTTGTATGATAATGCAATGTGCAAGGAAATTATTCCAACTAGTGAATTTATTAACAGTAAG TTAACAGCAAAAGCAAATAGGCAACTTCAAGATCCTTTAGTAATCATGACAGGAAACATCCCAACATGGC TTACTGAGCTAGGAAAAACCTGCCCATTTTTCTTTCCTTTTGATACCCGGCAAATGCTTTTTTATGTAAC TGCATTTGATCGGGACCGAGCAATGCAAAGATTACTTGATACCAACCCAGAAATCAACCAGTCTGATTCT CAAGATAGCAGAGTTGCACCTAGATTGGATAGAAAAAAACGTACTGTGAACCGAGAGGAGCTGCTGAAAC AGGCGGAGTCTGTGATGCAGGACCTCGGCAGCTCACGGGCCATGTTAGAAATCCAGTATGAAAATGAGGT TGGTACAGGTCTTGGGCCTACACTGGAGTTTTATGCGCTTGTATCTCAGGAACTACAGAGAGCTGACTTG GGTCTTTGGAGAGGTGAAGAAGTAACTCTTAGCAATCCAAAAGGGAGCCAAGAAGGGACCAAGTATATTC AAAACCTCCAGGGCCTGTTTGCGCTTCCCTTTGGTAGGACAGCAAAGCCAGCTCATATCGCAAAGGTTAA GATGAAGTTTCGCTTCTTAGGAAAATTAATGGCCAAGGCTATCATGGATTTCAGATTGGTGGACCTTCCC CTTGGCTTACCCTTTTATAAATGGATGCTACGGCAAGAAACTTCACTGACATCACACGATTTGTTTGACA TCGACCCAGTTGTAGCCAGATCAGTTTATCACCTAGAAGACATTGTCAGACAGAAGAAAAGACTTGAACA AGATAAATCCCAGACCAAAGAGAGTCTACAGTATGCATTAGAAACCTTGACTATGAATGGCTGCTCAGTT GAAGATCTAGGACTGGATTTCACTCTGCCAGGGTTTCCCAATATCGAACTGAAGAAAGGAGGGAAGGATA TACCAGTCACTATCCACAATTTAGAGGAGTATCTAAGACTGGTTATATTCTGGGCACTAAATGAAGGCGT TTCTAGGCAATTTGATTCGTTCAGAGATGGATTTGAATCAGTCTTCCCACTCAGTCATCTTCAGTACTTC TACCCGGAGGAACTGGATCAGCTCCTTTGTGGCAGTAAAGCAGACACTTGGGATGCAAAGACACTGATGG AATGCTGTAGGCCTGATCATGGTTATACTCATGACAGTCGGGCTGTGAAGTTTTTGTTTGAGATTCTCAG TAGTTTTGATAATGAGCAGCAGAGGTTATTTCTCCAGTTTGTGACTGGTAGCCCAAGATTGCCTGTTGGA GGATTCCGGAGTTTGAATCCACCTTTGACAATTGTCCGAAAGACGTTTGAATCAACAGAAAACCCAGATG ACTTCTTGCCCTCTGTAATGACTTGTGTGAACTATCTTAAGTTGCCGGACTATTCAAGCATTGAGATAAT GCGTGAAAAACTGTTGATAGCAGCAAGAGAAGGGCAGCAGTCGTTCCATCTTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240227 representing NM_001284215 Red=Cloning site Green=Tags(s) MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSR GVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGA TGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVA SASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLP GSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEA RRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPH LFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPA LITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHS KAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCL CFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNI AETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQ WRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSN TSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDV LKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKAC TNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSP PRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNRE KIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFE IQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLS QMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQA IERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFS IQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDEL WHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSK LTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDS QDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADL GLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLP LGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSV EDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYF YPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVG GFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284215
ORF Size	6075 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284215.2
RefSeq Size	9861 bp
RefSeq ORF	6078 bp
Locus ID	9320
UniProt ID	Q14669
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
MW	224.4 kDa
Gene Summary	The protein encoded by this gene is an E3 ubiquitin-protein ligase involved in the degradation of the p19ARF/ARF isoform of CDKN2A, a tumor suppressor. The encoded protein also plays a role in the DNA damage response by regulating the stability of USP7, which regulates tumor suppressor p53. [provided by RefSeq, Jan 2017]

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