TRIP12 (NM_001284216) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MRD49; TRIP-12; TRIPC; ULF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240204 representing NM_001284216 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAACCGGCCTAATAACAATCCAGGGGGGTCACTGCGACGTTCACAGAGGAACACTGCCGGGGCCC AACCACAAGACGACTCAATAGGAGGAAGTCGGCGAGGCTCTGGCCTGGGCAAAAGAGGAGCAGCTGAAGC TCGTCGACAGGAGAAAATGGCAGACCCTGAAAGCAACCAGGAGGCAGTAAATTCTTCAGCTGCTCGGACA GATGAAGCTCCCCAAGGAGCTGCAGCCTCTAGTTCTGTTGCAGGGGCTGTTGGCATGACCACCTCTGGGG AGAGTGAATCAGATGATTCCGAGATGGGACGTTTGCAAGCTTTGTTAGAGGCAAGGGGTCTTCCCCCTCA CCTATTTGGTCCTCTTGGTCCTCGGATGTCACAGCTTTTCCATAGAACAATTGGAAGTGGAGCTAGTTCT AAGGCCCAGCAGCTACTACAAGGATTGCAAGCCAGTGATGAAAGTCAACAGCTTCAGGCAGTTATTGAGA TGTGTCAGTTACTGGTCATGGGAAATGAGGAGACACTGGGAGGGTTTCCTGTCAAGAGTGTTGTTCCAGC TTTGATTACGTTACTTCAGATGGAGCACAATTTTGATATTATGAACCATGCTTGTCGAGCCTTAACATAC ATGATGGAAGCACTTCCTCGATCTTCTGCTGTTGTAGTAGATGCTATTCCTGTCTTTTTAGAAAAGCTGC AAGTTATTCAGTGTATTGATGTGGCAGAGCAGGCCTTGACTGCCTTGGAGATGTTGTCACGGAGACATAG TAAAGCCATTCTACAGGCGGGTGGTTTGGCAGACTGCTTGCTGTACCTAGAATTCTTCAGCATAAATGCC CAAAGAAATGCATTAGCAATTGCAGCTAATTGCTGCCAGAGTATCACGCCAGATGAATTTCATTTTGTGG CAGATTCACTCCCATTGCTAACCCAAAGGCTAACACATCAGGATAAAAAGTCAGTAGAAAGCACTTGCCT TTGTTTTGCACGCCTAGTGGACAACTTCCAGCATGAGGAGAATTTACTCCAGCAGGTTGCTTCCAAAGAT CTGCTTACAAATGTTCAACAGCTGTTGGTAGTGACTCCACCCATTTTAAGTTCTGGGATGTTTATAATGG TGGTTCGCATGTTTTCTCTGATGTGTTCCAACTGTCCAACTTTAGCTGTTCAACTTATGAAACAAAACAT TGCAGAAACGCTTCACTTTCTCCTGTGTGGTGCCTCCAATGGAAGTTGTCAGGAACAGATTGATCTTGTT CCACGAAGCCCTCAAGAGTTGTATGAACTGACATCTCTGATTTGTGAACTTATGCCATGTTTACCAAAAG AAGGCATTTTTGCAGTTGATACCATGTTGAAGAAGGGAAATGCACAGAACACAGATGGTGCGATATGGCA GTGGCGTGATGATCGGGGCCTCTGGCATCCATATAACAGGATTGACAGCCGGATCATTGAGGCAGCCCAT CAGGTCGGTGAGGATGAGATAAGCTTGTCCACTCTGGGACGAGTTTATACTATTGATTTTAATTCTATGC AGCAAATCAATGAGGACACGGGAACAGCACGTGCCATTCAGAGAAAACCTAACCCGTTAGCCAATAGTAA CACTAGTGGATATTCAGAGTCAAAGAAGGATGATGCTCGAGCACAGCTTATGAAAGAGGATCCGGAACTG GCTAAGTCTTTTATTAAGACATTATTTGGTGTTCTTTATGAAGTGTATAGTTCCTCAGCAGGACCTGCGG TCAGACATAAGTGCCTTAGAGCAATTCTTAGGATAATTTATTTTGCGGATGCTGAACTTCTGAAGGATGT TCTGAAAAATCATGCTGTTTCAAGTCACATTGCTTCCATGCTGTCAAGCCAAGACCTGAAGATAGTAGTG GGAGCACTTCAGATGGCAGAAATTTTAATGCAGAAGTTACCTGATATTTTTAGTGTTTACTTCAGAAGAG AAGGTGTAATGCATCAAGTAAAACACTTAGCAGAATCAGAGTCTTTGTTGACAAGTCCACCAAAGGCATG TACGAATGGATCGGGATCCATGGGATCCACAACTTCAGTCAGCAGTGGGACAGCCACAGCTGCCACTCAT GCTGCAGCTGACTTGGGATCACCCAGCTTGCAGCACAGCAGGGATGATTCTTTAGATCTCAGCCCTCAAG GTCGATTAAGTGATGTTCTAAAGAGAAAACGACTGCCAAAACGAGGGCCAAGAAGGCCAAAGTACTCACC TCCAAGAGATGATGACAAAGTAGACAATCAAGCTAAAAGCCCCACCACTACTCAGTCACCTAAATCTTCT TTCCTGGCAAGCTTGAATCCAAAAACATGGGGAAGGTTAAGTACACAGTCCAACAGCAACAACATTGAGC CAGCACGGACTGCGGGAGGTAGTGGCCTTGCCAGGGCTGCCTCAAAGGATACCATCTCCAATAATAGAGA AAAAATTAAAGGTTGGATTAAGGAGCAGGCACATAAATTTGTAGAACGTTATTTCAGTTCTGAGAATATG GATGGAAGCAACCCTGCATTGAATGTCCTTCAGAGACTTTGTGCTGCAACCGAACAACTCAACCTCCAGG TGGATGGTGGAGCTGAGTGCCTTGTAGAAATCCGTAGCATAGTCTCAGAGTCAGATGTTTCATCATTTGA AATCCAACATAGTGGATTTGTGAAGCAGCTGTTGCTTTATTTGACATCTAAAAGTGAAAAGGATGCTGTG AGCAGAGAGATCAGATTAAAGCGATTTCTTCATGTATTTTTTTCTTCTCCACTTCCTGGAGAAGAGCCCA TTGGAAGAGTGGAACCAGTGGGTAATGCACCTTTGTTGGCATTAGTTCACAAGATGAACAACTGCCTCAG CCAGATGGAACAATTTCCAGTCAAAGTACATGATTTCCCTAGTGGAAATGGGACAGGAGGCAGCTTTTCT CTCAACAGAGGATCACAGGCTTTAAAATTTTTCAACACACATCAATTAAAATGCCAGTTACAAAGGCATC CAGACTGTGCAAATGTGAAGCAGTGGAAGGGTGGACCTGTCAAGATTGACCCTCTGGCTTTGGTACAAGC CATCGAGAGATACCTTGTAGTTAGAGGGTATGGAAGAGTAAGAGAAGATGATGAAGACAGCGATGACGAT GGATCAGATGAGGAAATAGATGAGTCTCTGGCTGCTCAGTTCCTAAATTCAGGAAATGTAAGACACAGGC TGCAGTTTTATATTGGAGAACATTTGCTGCCGTATAACATGACTGTGTATCAGGCAGTACGGCAGTTTAG TATACAGGCTGAAGATGAAAGAGAATCCACAGATGATGAGAGCAATCCTCTAGGCAGAGCTGGTATTTGG ACAAAGACTCATACAATATGGTATAAACCTGTGAGAGAGGATGAAGAAAGTAATAAAGATTGTGTTGGTG GTAAAAGAGGAAGAGCCCAAACAGCTCCAACGAAAACTTCCCCTAGAAATGCAAAAAAGCATGATGAGTT ATGGCACGATGGAGTGTGCCCATCAGTATCAAATCCTTTAGAAGTTTACCTCATTCCCACACCACCTGAA AATATAACATTTGAAGACCCGTCATTAGATGTGATCCTTCTTTTAAGAGTTTTACATGCTATCAGTCGAT ACTGGTATTACTTGTATGATAATGCAATGTGCAAGGAAATTATTCCAACTAGTGAATTTATTAACAGTAA GTTAACAGCAAAAGCAAATAGGCAACTTCAAGATCCTTTAGTAATCATGACAGGAAACATCCCAACATGG CTTACTGAGCTAGGAAAAACCTGCCCATTTTTCTTTCCTTTTGATACCCGGCAAATGCTTTTTTATGTAA CTGCATTTGATCGGGACCGAGCAATGCAAAGATTACTTGATACCAACCCAGAAATCAACCAGTCTGATTC TCAAGATAGCAGAGTTGCACCTAGATTGGATAGAAAAAAACGTACTGTGAACCGAGAGGAGCTGCTGAAA CAGGCGGAGTCTGTGATGCAGGACCTCGGCAGCTCACGGGCCATGTTAGAAATCCAGTATGAAAATGAGG TTGGTACAGGTCTTGGGCCTACACTGGAGTTTTATGCGCTTGTATCTCAGGAACTACAGAGAGCTGACTT GGGTCTTTGGAGAGGTGAAGAAGTAACTCTTAGCAATCCAAAAGGGAGCCAAGAAGGGACCAAGTATATT CAAAACCTCCAGGGCCTGTTTGCGCTTCCCTTTGGTAGGACAGCAAAGCCAGCTCATATCGCAAAGGTTA AGATGAAGTTTCGCTTCTTAGGAAAATTAATGGCCAAGGCTATCATGGATTTCAGATTGGTGGACCTTCC CCTTGGCTTACCCTTTTATAAATGGATGCTACGGCAAGAAACTTCACTGACATCACACGATTTGTTTGAC ATCGACCCAGTTGTAGCCAGATCAGTTTATCACCTAGAAGACATTGTCAGACAGAAGAAAAGACTTGAAC AAGATAAATCCCAGACCAAAGAGAGTCTACAGTATGCATTAGAAACCTTGACTATGAATGGCTGCTCAGT TGAAGATCTAGGACTGGATTTCACTCTGCCAGGGTTTCCCAATATCGAACTGAAGAAAGGAGGGAAGGAT ATACCAGTCACTATCCACAATTTAGAGGAGTATCTAAGACTGGTTATATTCTGGGCACTAAATGAAGGCG TTTCTAGGCAATTTGATTCGTTCAGAGATGGATTTGAATCAGTCTTCCCACTCAGTCATCTTCAGTACTT CTACCCGGAGGAACTGGATCAGCTCCTTTGTGGCAGTAAAGCAGACACTTGGGATGCAAAGACACTGATG GAATGCTGTAGGCCTGATCATGGTTATACTCATGACAGTCGGGCTGTGAAGTTTTTGTTTGAGATTCTCA GTAGTTTTGATAATGAGCAGCAGAGGTTATTTCTCCAGTTTGTGACTGGTAGCCCAAGATTGCCTGTTGG AGGATTCCGGAGTTTGAATCCACCTTTGACAATTGTCCGAAAGACGTTTGAATCAACAGAAAACCCAGAT GACTTCTTGCCCTCTGTAATGACTTGTGTGAACTATCTTAAGTTGCCGGACTATTCAAGCATTGAGATAA TGCGTGAAAAACTGTTGATAGCAGCAAGAGAAGGGCAGCAGTCGTTCCATCTTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240204 representing NM_001284216 Red=Cloning site Green=Tags(s) MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAART DEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASS KAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTY MMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINA QRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKD LLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLV PRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAH QVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPEL AKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVV GALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATH AAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSS FLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENM DGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAV SREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFS LNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDD GSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIW TKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPE NITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTW LTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLK QAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYI QNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFD IDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKD IPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLM ECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPD DFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284216
ORF Size	5166 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284216.2
RefSeq Size	9082 bp
RefSeq ORF	5169 bp
Locus ID	9320
UniProt ID	Q14669
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
MW	192.5 kDa
Gene Summary	The protein encoded by this gene is an E3 ubiquitin-protein ligase involved in the degradation of the p19ARF/ARF isoform of CDKN2A, a tumor suppressor. The encoded protein also plays a role in the DNA damage response by regulating the stability of USP7, which regulates tumor suppressor p53. [provided by RefSeq, Jan 2017]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.