NEDD4 (NM_001284339) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NEDD4-1; RPF1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240103 representing NM_001284339 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACAAAGCTTACGATTGCACTTTGCAGCCAGAAGAAGCAATACTTACCCTTTGTCAGAAACCTCCG GAGATGACTTGGATAGCCATGTTCACATGTGCTTCAAAAGACCAACACGGATTTCAACGTCTAACGTTGT TCAAATGAAGCTGACTCCCAGACAGACTGCACTAGCTCCGTTAATAAAGGAAAACGTTCAGTCTCAAGAA AGATCATCTGTTCCCTCATCTGAAAATGTTAATAAAAAGAGCAGCTGTCTACAGATTTCACTACAGCCAA CAAGGTACAGTGGATATCTTCAGTCTAGCAATGTCTTAGCTGATAGTGATGATGCTTCGTTTACTTGTAT CTTGAAGGATGGTATTTACAGTAGTGCTGTGGTCGATAATGAATTGAATGCTGTGAATGATGGTCACCTT GTAAGCAGTCCAGCCATTTGTAGTGGTAGCCTTAGTAACTTTTCAACCAGTGATAATGGGTCTTACAGCA GCAACGGTAGTGATTTTGGGTCATGTGCAAGTATCACAAGTGGAGGTTCATATACTAACAGTGTCATCAG TGACAGTAGTAGTTATACTTTTCCACCAAGTGATGATACTTTTTTGGGTGGAAACTTACCTTCTGACAGC ACCTCCAATAGAAGTGTGCCAAACAGGAATACTACTCCTTGTGAAATTTTTTCAAGAAGTACAAGTACAG ATCCTTTTGTCCAGGATGACTTGGAACATGGATTAGAGATTATGAAATTGCCAGTGAGCAGGAACACAAA AATTCCACTAAAACGTTACTCCTCCTTAGTCATTTTTCCTAGGAGTCCTTCAACTACCCGACCGACTTCT CCAACAAGTCTGTGTACTCTTCTGAGCAAAGGATCCTATCAAACTTCACACCAGTTTATTATTTCTCCTA GTGAAATTGCACATAATGAGGATGGCACTAGTGCTAAAGGATTTCTTTCAACAGCTGTCAATGGACTTCG GTTATCTAAAACAATTTGTACCCCCGGAGAAGTAAGAGACATACGGCCGCTTCACAGGAAGGGCTCGTTA CAGAAGAAAATTGTTCTTTCGAATAATACTCCCAGACAGACTGTCTGTGAAAAGTCATCTGAAGGATATT CTTGTGTTTCAGTGCATTTCACCCAACGAAAAGCAGCTACATTAGACTGTGAAACAACAAATGGTGATTG TAAACCAGAAATGTCAGAAATTAAGCTTAATTCTGATTCAGAGTATATTAAGCTCATGCATAGGACATCT GCATGTTTGCCATCCTCCCAAAATGTAGATTGTCAAATAAATATCAATGGAGAATTGGAAAGACCACATT CACAGATGAACAAAAACCATGGTATTTTACGAAGAAGTATTTCATTGGGAGGAGCTTATCCAAATATTTC CTGTCTATCCAGCCTTAAGCACAATTGTTCTAAAGGGGGACCATCTCAGTTACTCATAAAGTTTGCATCT GGAAATGAAGGTAAAGTGGATAATTTATCAAGAGACAGCAACAGAGATTGCACAAATGAACTGTCTAATT CTTGCAAGACAAGAGATGATTTCCTAGGTCAAGTGGATGTTCCACTTTATCCATTACCGACAGAAAATCC AAGATTGGAGAGACCATATACATTTAAGGATTTTGTTCTTCATCCAAGAAGTCACAAATCAAGAGTTAAA GGTTATCTGAGACTAAAAATGACTTATTTACCTAAAACCAGTGGCTCAGAAGATGATAATGCAGAACAGG CTGAGGAATTAGAGCAACAACAAGAACCTTCTCCTCTACCTCCAGGGTGGGAAGAGAGGCAGGATATCCT TGGAAGGACCTATTATGTAAACCATGAATCTAGAAGAACACAGTGGAAAAGACCAACCCCTCAGGACAAC CTAACAGATGCTGAGAATGGCAACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGGCAGATAT CCGAGGAAACAGAAAGTGTTGACAACCGAGAGTCTTCCGAGAACTGGGAAATTATAAGAGAAGATGAAGC CACCATGTATAGCAACCAGGCCTTCCCATCACCTCCACCGTCAAGTAACTTGGATGTTCCAACTCATCTT GCAGAAGAATTGAATGCCAGACTCACCATTTTTGGAAATTCAGCCGTGAGCCAGCCAGCATCGAGCTCAA ATCATTCCAGCAGAAGAGGCAGCTTACAAGCCTATACTTTTGAGGAACAACCTACACTTCCTGTGCTTTT GCCTACTTCATCTGGATTACCACCAGGTTGGGAAGAAAAACAAGATGAAAGAGGAAGATCATATTATGTA GATCACAATTCCAGAACGACTACTTGGACAAAGCCCACTGTACAGGCCACAGTGGAGACCAGTCAGCTGA CCTCAAGCCAGAGTTCTGCAGGCCCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACCCA GCCATCTGAAATTGAGCAAGGATTCCTTCCTAAAGGCTGGGAAGTCCGGCATGCACCAAATGGGAGGCCT TTCTTTATTGACCACAACACTAAAACCACCACCTGGGAAGATCCAAGATTGAAAATTCCAGCCCATCTGA GAGGAAAGACATCACTTGATACTTCCAATGATCTAGGGCCTTTACCTCCAGGATGGGAAGAGAGAACTCA CACAGATGGAAGAATCTTCTACATAAATCACAATATAAAAAGAACACAATGGGAAGATCCTCGGTTGGAG AATGTAGCAATAACTGGACCAGCAGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAA GAAAGTTGAAGAAGCAGAATGACATTCCAAACAAATTTGAAATGAAACTTCGCCGAGCAACTGTTCTTGA AGACTCTTACCGGAGAATTATGGGTGTCAAGAGAGCAGACTTCCTGAAGGCTCGACTGTGGATTGAGTTT GATGGTGAAAAGGGATTGGATTATGGAGGAGTTGCCAGAGAATGGTTCTTCCTGATCTCAAAGGAAATGT TTAACCCTTATTATGGGTTGTTTGAATATTCTGCTACGGACAATTATACCCTACAGATAAATCCAAACTC TGGATTGTGTAACGAAGATCACCTCTCTTACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTAT CATGGCAAACTGTTGGATGGTTTTTTCATCCGCCCATTTTACAAGATGATGCTTCACAAACCAATAACCC TTCATGATATGGAATCTGTGGATAGTGAATATTACAATTCCCTAAGATGGATTCTTGAAAATGACCCAAC AGAATTGGACCTCAGGTTTATCATAGATGAAGAACTTTTTGGACAGACACATCAACATGAGCTGAAAAAT GGTGGATCAGAAATAGTTGTCACCAATAAGAACAAAAAGGAATATATTTATCTTGTAATACAATGGCGAT TTGTAAACCGAATCCAGAAGCAAATGGCTGCTTTTAAAGAGGGATTCTTTGAACTAATACCACAGGATCT CATCAAAATTTTTGATGAAAATGAACTAGAGCTTCTTATGTGTGGACTGGGAGATGTTGATGTGAATGAC TGGAGGGAACATACAAAGTATAAAAATGGCTACAGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGG CTGTTTTAATGATGGATTCAGAAAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTCGGGTGCC TATGAATGGATTTGCTGAACTATACGGTTCAAATGGACCACAGTCATTTACAGTTGAACAGTGGGGTACT CCTGAAAAGCTGCCAAGAGCTCATACCTGTTTTAATCGCCTGGACTTGCCACCTTATGAATCATTTGAAG AATTATGGGATAAACTTCAGATGGCAATTGAAAACACCCAGGGCTTTGATGGAGTTGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240103 representing NM_001284339 Red=Cloning site Green=Tags(s) MAQSLRLHFAARRSNTYPLSETSGDDLDSHVHMCFKRPTRISTSNVVQMKLTPRQTALAPLIKENVQSQE RSSVPSSENVNKKSSCLQISLQPTRYSGYLQSSNVLADSDDASFTCILKDGIYSSAVVDNELNAVNDGHL VSSPAICSGSLSNFSTSDNGSYSSNGSDFGSCASITSGGSYTNSVISDSSSYTFPPSDDTFLGGNLPSDS TSNRSVPNRNTTPCEIFSRSTSTDPFVQDDLEHGLEIMKLPVSRNTKIPLKRYSSLVIFPRSPSTTRPTS PTSLCTLLSKGSYQTSHQFIISPSEIAHNEDGTSAKGFLSTAVNGLRLSKTICTPGEVRDIRPLHRKGSL QKKIVLSNNTPRQTVCEKSSEGYSCVSVHFTQRKAATLDCETTNGDCKPEMSEIKLNSDSEYIKLMHRTS ACLPSSQNVDCQININGELERPHSQMNKNHGILRRSISLGGAYPNISCLSSLKHNCSKGGPSQLLIKFAS GNEGKVDNLSRDSNRDCTNELSNSCKTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSRVK GYLRLKMTYLPKTSGSEDDNAEQAEELEQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDN LTDAENGNIQLQAQRAFTTRRQISEETESVDNRESSENWEIIREDEATMYSNQAFPSPPPSSNLDVPTHL AEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAYTFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYV DHNSRTTTWTKPTVQATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHAPNGRP FFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEERTHTDGRIFYINHNIKRTQWEDPRLE NVAITGPAVPYSRDYKRKYEFFRRKLKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEF DGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVY HGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYYNSLRWILENDPTELDLRFIIDEELFGQTHQHELKN GGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVND WREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGT PEKLPRAHTCFNRLDLPPYESFEELWDKLQMAIENTQGFDGVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284339
ORF Size	3909 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284339.1, NP_001271268.1
RefSeq Size	7192 bp
RefSeq ORF	3912 bp
Locus ID	4734
UniProt ID	P46934
Protein Families	Druggable Genome
Protein Pathways	Endocytosis, Ubiquitin mediated proteolysis
MW	147.8 kDa
Gene Summary	This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016]

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