MTR (NM_001291939) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	cblG; HMAG; MS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240049 representing NM_001291939 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCACCCGCGCTCCAAGACCTGTCGCAACCCGAAGGTCTGAAGAAAACCCTGCGGGATGAGATCAATG CCATTCTGCAGAAGAGGATTATGGTGCTGGATGGAGGGATGGGGACCATGATCCAGCGGGAGAAGCTAAA CGAAGAACACTTCCGAGGTCAGGAATTTAAAGATCATGCCAGGCCGCTGAAAGGCAACAATGACATTTTA AGTATAACTCAGCCTGATGTCATTTACCAAATCCATAAGGAATACTTGCTGGCTGGGGCAGATATCATTG AAACAAATACTTTTAGCAGCACTAGTATTGCCCAAGCTGACTATGGCCTTGAACACTTGGCCTACCGGAT GAACATGTGCTCTGCAGGAGTGGCCAGAAAAGCTGCCGAGGAGGTAACTCTCCAGACAGGAATTAAGAGG TTTGTGGCAGGGGCTCTGGGTCCGACTAATAAGACACTCTCTGTGTCCCCATCTGTGGAAAGGCCGGATT ATAGGAACATCACATTTGATGAGCTTGTTGAAGCATACCAAGAGCAGGCCAAAGGACTTCTGGATGGCGG GGTTGATATCTTACTCATTGAAACTATTTTTGATACTGCCAATGCCAAGGCAGCCTTGTTTGCACTCCAA AATCTTTTTGAGGAGAAATATGCTCCCCGGCCTATCTTTATTTCAGGGACGATCGTTGATAAAAGTGGGC GGACTCTTTCCGGACAGACAGGAGAGGGATTTGTCATCAGCGTGTCTCATGGAGAACCACTCTGCATTGG ATTAAATTGTGCTTTGGGTGCAGCTGAAATGAGACCTTTTATTGAAATAATTGGAAAATGTACAACAGCC TATGTCCTCTGTTATCCCAATGCAGGTCTTCCCAACACCTTTGGTGACTATGATGAAACGCCTTCTATGA TGGCCAAGCACCTAAAGGATTTTGCTATGGATGGCTTGGTCAATATAGTTGGAGGATGCTGTGGGTCAAC ACCAGATCATATCAGGGAAATTGCTGAAGCTGTGAAAAATTGTAAGCCTAGAGTTCCACCTGCCACTGCT TTTGAAGGACATATGTTACTGTCTGGTCTAGAGCCCTTCAGGATTGGACCGTACACCAACTTTGTTAACA TTGGAGAGCGCTGTAATGTTGCAGGATCAAGGAAGTTTGCTAAACTCATCATGGCAGGAAACTATGAAGA AGCCTTGTGTGTTGCCAAAGTGCAGGTGGAAATGGGAGCCCAGGTGTTGGATGTCAACATGGATGATGGC ATGCTAGATGGTCCAAGTGCAATGACCAGATTTTGCAACTTAATTGCTTCCGAGCCAGACATCGCAAAGG TACCTTTGTGCATCGACTCCTCCAATTTTGCTGTGATTGAAGCTGGGTTAAAGTGCTGCCAAGGGAAGTG CATTGTCAATAGCATTAGTCTGAAGGAAGGAGAGGACGACTTCTTGGAGAAGGCCAGGAAGATTAAAAAG TATGGAGCTGCTATGGTGGTCATGGCTTTTGATGAAGAAGGACAGGCAACAGAAACAGACACAAAAATCA GAGTGTGCACCCGGGCCTACCATCTGCTTGTGAAAAAACTGGGCTTTAATCCAAATGACATTATTTTTGA CCCTAATATCCTAACCATTGGGACTGGAATGGAGGAACACAACTTGTATGCCATTAATTTTATCCATGCA ACAAAAGTCATTAAAGAAACATTACCTGGAGCCAGAATAAGTGGAGGTCTTTCCAACTTGTCCTTCTCCT TCCGAGGAATGGAAGCCATTCGAGAAGCAATGCATGGGGTTTTCCTTTACCATGCAATCAAGTCTGGCAT GGACATGGGGATAGTGAATGCTGGAAACCTCCCTGTGTATGATGATATCCATAAGGAACTTCTGCAGCTC TGTGAAGATCTCATCTGGAATAAAGACCCTGAGGCCACTGAGAAGCTCTTACGTTATGCCCAGACTCAAG GCACAGGAGGGAAGAAAGTCATTCAGACTGATGAGTGGAGAAATGGCCCTGTCGAAGAACGCCTTGAGTA TGCCCTTGTGAAGGTTATAAAGTCAGCCCGGGTTATGAAGAAGGCTGTTGGCCACCTTATCCCTTTCATG GAAAAAGAAAGAGAAGAAACCAGAGTGCTTAACGGCACAGTAGAAGAAGAGGACCCTTACCAGGGCACCA TCGTGCTGGCCACTGTTAAAGGCGACGTGCACGACATAGGCAAGAACATAGTTGGAGTAGTCCTTGGCTG CAATAATTTCCGAGTTATTGATTTAGGAGTCATGACTCCATGTGATAAGATACTGAAAGCTGCTCTTGAC CACAAAGCAGATATAATTGGCCTGTCAGGACTCATCACTCCTTCCCTGGATGAAATGATTTTTGTTGCCA AGGAAATGGAGAGATTAGCTATAAGGATTCCATTGTTGATTGGAGGAGCAACCACTTCAAAAACCCACAC AGCAGTTAAAATAGCTCCGAGATACAGTGCACCTGTAATCCATGTCCTGGACGCGTCCAAGAGTGTGGTG GTGTGTTCCCAGCTGTTAGATGAAAATCTAAAGGATGAATACTTTGAGGAAATCATGGAAGAATATGAAG ATATTAGACAGGACCATTATGAGTCTCTCAAGGAGAGGAGATACTTACCCTTAAGTCAAGCCAGAAAAAG TGGTTTCCAAATGGATTGGCTGTCTGAACCTCACCCAGTGAAGCCCACGTTTATTGGGACCCAGGTCTTT GAAGACTATGACCTGCAGAAGCTGGTGGACTACATTGACTGGAAGCCTTTCTTTGATGTCTGGCAGCTCC GGGGCAAGTACCCGAATCGAGGCTTTCCCAAGATATTTAACGACAAAACAGTAGGTGGAGAGGCCAGGAA GGTCTACGATGATGCCCACAATATGCTGAACACACTGATTAGTCAAAAGAAACTCCGGGCCCGGGGTGTG GTTGGGTTCTGGCCAGCACAGAGTATCCAAGACGACATTCACCTGTACGCAGAGGCTGCTGTGCCCCAGG CTGCAGAGCCCATAGCCACCTTCTATGGGTTAAGGCAACAGGCTGAGAAGGACTCTGCCAGCACGGAGCC ATACTACTGCCTCTCAGACTTCATCGCTCCCTTGCATTCTGGCATCCGTGACTACCTGGGCCTGTTTGCC GTTGCCTGCTTTGGGGTAGAAGAGCTGAGCAAGGCCTATGAGGATGATGGTGACGACTACAGCAGCATCA TGGTCAAGGCGCTGGGGGACCGGCTGGCAGAGGCCTTTGCAGAAGAGCTCCATGAAAGAGTTCGCCGAGA ACTGTGGGCCTACTGTGGCAGTGAGCAGCTGGACGTCGCAGACCTGCGCAGGCTGCGGTACAAGGGCATC CGCCCGGCTCCTGGCTACCCCAGCCAGCCCGACCACACCGAGAAGCTCACCATGTGGAGACTCGCAGACA TCGAGCAGTCTACAGGCATTAGGTTAACAGAATCATTAGCAATGGCACCTGCTTCAGCAGTCTCAGGCCT CTACTTCTCCAATTTGAAGTCCAAATATTTTGCTGTGGGGAAGATTTCCAAGGATCAGGTTGAGGATTAT GCATTGAGGAAGAACATATCTGTGGCTGAGGTTGAGAAATGGCTTGGACCCATTTTGGGATATGATACAG AC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240049 representing NM_001291939 Red=Cloning site Green=Tags(s) MSPALQDLSQPEGLKKTLRDEINAILQKRIMVLDGGMGTMIQREKLNEEHFRGQEFKDHARPLKGNNDIL SITQPDVIYQIHKEYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNMCSAGVARKAAEEVTLQTGIKR FVAGALGPTNKTLSVSPSVERPDYRNITFDELVEAYQEQAKGLLDGGVDILLIETIFDTANAKAALFALQ NLFEEKYAPRPIFISGTIVDKSGRTLSGQTGEGFVISVSHGEPLCIGLNCALGAAEMRPFIEIIGKCTTA YVLCYPNAGLPNTFGDYDETPSMMAKHLKDFAMDGLVNIVGGCCGSTPDHIREIAEAVKNCKPRVPPATA FEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRKFAKLIMAGNYEEALCVAKVQVEMGAQVLDVNMDDG MLDGPSAMTRFCNLIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKIKK YGAAMVVMAFDEEGQATETDTKIRVCTRAYHLLVKKLGFNPNDIIFDPNILTIGTGMEEHNLYAINFIHA TKVIKETLPGARISGGLSNLSFSFRGMEAIREAMHGVFLYHAIKSGMDMGIVNAGNLPVYDDIHKELLQL CEDLIWNKDPEATEKLLRYAQTQGTGGKKVIQTDEWRNGPVEERLEYALVKVIKSARVMKKAVGHLIPFM EKEREETRVLNGTVEEEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALD HKADIIGLSGLITPSLDEMIFVAKEMERLAIRIPLLIGGATTSKTHTAVKIAPRYSAPVIHVLDASKSVV VCSQLLDENLKDEYFEEIMEEYEDIRQDHYESLKERRYLPLSQARKSGFQMDWLSEPHPVKPTFIGTQVF EDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFNDKTVGGEARKVYDDAHNMLNTLISQKKLRARGV VGFWPAQSIQDDIHLYAEAAVPQAAEPIATFYGLRQQAEKDSASTEPYYCLSDFIAPLHSGIRDYLGLFA VACFGVEELSKAYEDDGDDYSSIMVKALGDRLAEAFAEELHERVRRELWAYCGSEQLDVADLRRLRYKGI RPAPGYPSQPDHTEKLTMWRLADIEQSTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQVEDY ALRKNISVAEVEKWLGPILGYDTD SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291939
ORF Size	3642 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291939.1, NP_001278868.1
RefSeq Size	10422 bp
RefSeq ORF	3645 bp
Locus ID	4548
UniProt ID	Q99707
Protein Families	Druggable Genome
Protein Pathways	Cysteine and methionine metabolism, Metabolic pathways, One carbon pool by folate
MW	135.2 kDa
Gene Summary	This gene encodes the 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

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