LPHN2 (ADGRL2) (NM_001297705) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CIRL2; CL2; LEC1; LPHH1; LPHN2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC240025 representing NM_001297705 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCTTCTGGTTGCAGAATGCGAAGTCTGTGGTTTATCATTGTAATCAGCTTCTTACCAAATACAG AAGGTTTCAGCAGAGCAGCTTTACCATTTGGGCTGGTGAGGCGAGAATTATCCTGTGAAGGTTATTCTAT AGATCTGCGATGCCCGGGCAGTGATGTCATCATGATTGAGAGCGCTAACTATGGTCGGACGGATGACAAG ATTTGTGATGCTGACCCATTTCAGATGGAGAATACAGACTGCTACCTCCCCGATGCCTTCAAAATTATGA CTCAAAGGTGCAACAATCGAACACAGTGTATAGTAGTTACTGGGTCAGATGTGTTTCCTGATCCATGTCC TGGAACATACAAATACCTTGAAGTCCAATATGAATGTGTCCCTTACATTTTTGTGTGTCCTGGGACCTTG AAAGCAATTGTGGACTCACCATGTATATATGAAGCTGAACAAAAGGCGGGTGCTTGGTGCAAGGACCCTC TTCAGGCTGCAGATAAAATTTATTTCATGCCCTGGACTCCCTATCGTACCGATACTTTAATAGAATATGC TTCTTTAGAAGATTTCCAAAATAGTCGCCAAACAACAACATATAAACTTCCAAATCGAGTAGATGGTACT GGATTTGTGGTGTATGATGGTGCTGTCTTCTTTAACAAAGAAAGAACGAGGAATATTGTGAAATTTGACT TGAGGACTAGAATTAAGAGTGGCGAGGCCATAATTAACTATGCCAACTACCATGATACCTCACCATACAG ATGGGGAGGAAAGACTGATATCGACCTAGCAGTTGATGAAAATGGTTTATGGGTCATTTACGCCACTGAA CAGAACAATGGAATGATAGTTATTAGCCAGCTGAATCCATACACTCTTCGATTTGAAGCAACGTGGGAGA CTGTATACGACAAACGTGCCGCATCAAATGCTTTTATGATATGCGGAGTCCTCTATGTGGTTAGGTCAGT TTATCAAGACAATGAAAGTGAAACAGGCAAGAACTCAATTGATTACATTTATAATACCCGATTAAACCGA GGAGAATATGTAGATGTTCCCTTCCCCAACCAGTATCAGTATATTGCTGCAGTGGATTACAATCCAAGAG ATAACCAACTTTACGTGTGGAACAATAACTTCATTTTACGATATTCTCTGGAGTTTGGTCCACCTGATCC TGCCCAAGTGCCTACCACAGCTGTGACAATAACTTCTTCAGCTGAGCTGTTCAAAACCATAATATCAACC ACAAGCACTACTTCACAGAAAGGCCCCATGAGCACAACTGTAGCTGGATCACAGGAAGGAAGCAAAGGGA CAAAACCACCTCCAGCAGTTTCTACAACCAAAATTCCACCTATAACAAATATTTTTCCCCTGCCAGAGAG ATTCTGTGAAGCATTAGACTCCAAGGGGATAAAGTGGCCTCAGACACAAAGGGGAATGATGGTTGAACGA CCATGCCCTAAGGGAACAAGAGGAACTGCCTCATATCTCTGCATGATTTCCACTGGAACATGGAACCCTA AGGGCCCCGATCTTAGCAACTGTACCTCACACTGGGTGAATCAGCTGGCTCAGAAGATCAGAAGCGGAGA AAATGCTGCTAGTCTTGCCAATGAACTGGCTAAACATACCAAAGGGCCAGTGTTTGCTGGGGATGTAAGT TCTTCAGTGAGATTGATGGAGCAGTTGGTGGACATCCTTGATGCACAGCTGCAGGAACTGAAACCTAGTG AAAAAGATTCAGCTGGACGGAGTTATAACAAGGCAATTGTTGACACAGTGGACAACCTTCTGAGACCCGA AGCTTTGGAATCATGGAAACATATGAATTCTTCTGAACAAGCACATACTGCAACAATGTTACTCGATACA TTGGAAGAAGGAGCTTTTGTCCTAGCTGACAATCTTTTAGAACCAACAAGGGTCTCAATGCCCACAGAAA ATATTGTCCTGGAAGTTGCCGTACTCAGTACAGAAGGACAGATCCAAGACTTTAAATTTCCTCTGGGCAT CAAAGGAGCAGGCAGCTCAATCCAACTGTCCGCAAATACCGTCAAACAGAACAGCAGGAATGGGCTTGCA AAGTTGGTGTTCATCATTTACCGGAGCCTGGGACAGTTCCTTAGTACAGAAAATGCAACCATTAAACTGG GTGCTGATTTTATTGGTCGTAATAGCACCATTGCAGTGAACTCTCACGTCATTTCAGTTTCAATCAATAA AGAGTCCAGCCGAGTATACCTGACTGATCCTGTGCTTTTTACCCTGCCACACATTGATCCTGACAATTAT TTCAATGCAAACTGCTCCTTCTGGAACTACTCAGAGAGAACTATGATGGGATATTGGTCTACCCAGGGCT GCAAGCTGGTTGACACTAATAAAACTCGAACAACGTGTGCATGCAGCCACCTAACCAATTTTGCAATTCT CATGGCCCACAGGGAAATTGCATATAAAGATGGCGTTCATGAATTACTTCTTACAGTCATCACCTGGGTG GGAATTGTCATTTCCCTTGTTTGCCTGGCTATCTGCATCTTCACCTTCTGCTTTTTCCGTGGCCTACAGA GTGACCGAAATACTATTCACAAGAACCTTTGTATCAACCTTTTCATTGCTGAATTTATTTTCCTAATAGG CATTGATAAGACAAAATATGCGATTGCATGCCCAATATTTGCAGGACTTCTACACTTTTTCTTTTTGGCA GCTTTTGCTTGGATGTGCCTAGAAGGTGTGCAGCTCTACCTAATGTTAGTTGAAGTTTTTGAAAGTGAAT ATTCAAGGAAAAAATATTACTATGTTGCTGGTTACTTGTTTCCTGCCACAGTGGTTGGAGTTTCAGCTGC TATTGACTATAAGAGCTATGGAACAGAAAAAGCTTGCTGGCTTCATGTTGATAACTACTTTATATGGAGC TTCATTGGACCTGTTACCTTCATTATTCTGCTAAATATTATCTTCTTGGTGATCACATTGTGCAAAATGG TGAAGCATTCAAACACTTTGAAACCAGATTCTAGCAGGTTGGAAAACATTAAGTCTTGGGTGCTTGGCGC TTTCGCTCTTCTGTGTCTTCTTGGCCTCACCTGGTCCTTTGGGTTGCTTTTTATTAATGAGGAGACTATT GTGATGGCATATCTCTTCACTATATTTAATGCTTTCCAGGGAGTGTTCATTTTCATCTTTCACTGTGCTC TCCAAAAGAAAGTACGAAAAGAATATGGCAAGTGCTTCAGACACTCATACTGCTGTGGAGGCCTCCCAAC TGAGAGTCCCCACAGTTCAGTGAAGGCATCAACCACCAGAACCAGTGCTCGCTATTCCTCTGGCACACAG AGTCGTATAAGAAGAATGTGGAATGATACTGTGAGAAAACAATCAGAATCTTCTTTTATCTCAGGTGACA TCAATAGCACTTCAACACTTAATCAAGGACTGACATCACATGGTCTGAGAGCCCATCTTCAAGATTTATA TCATTTAGAGCTACTCTTAGGCCAGATAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC240025 representing NM_001297705 Red=Cloning site Green=Tags(s) MVSSGCRMRSLWFIIVISFLPNTEGFSRAALPFGLVRRELSCEGYSIDLRCPGSDVIMIESANYGRTDDK ICDADPFQMENTDCYLPDAFKIMTQRCNNRTQCIVVTGSDVFPDPCPGTYKYLEVQYECVPYIFVCPGTL KAIVDSPCIYEAEQKAGAWCKDPLQAADKIYFMPWTPYRTDTLIEYASLEDFQNSRQTTTYKLPNRVDGT GFVVYDGAVFFNKERTRNIVKFDLRTRIKSGEAIINYANYHDTSPYRWGGKTDIDLAVDENGLWVIYATE QNNGMIVISQLNPYTLRFEATWETVYDKRAASNAFMICGVLYVVRSVYQDNESETGKNSIDYIYNTRLNR GEYVDVPFPNQYQYIAAVDYNPRDNQLYVWNNNFILRYSLEFGPPDPAQVPTTAVTITSSAELFKTIIST TSTTSQKGPMSTTVAGSQEGSKGTKPPPAVSTTKIPPITNIFPLPERFCEALDSKGIKWPQTQRGMMVER PCPKGTRGTASYLCMISTGTWNPKGPDLSNCTSHWVNQLAQKIRSGENAASLANELAKHTKGPVFAGDVS SSVRLMEQLVDILDAQLQELKPSEKDSAGRSYNKAIVDTVDNLLRPEALESWKHMNSSEQAHTATMLLDT LEEGAFVLADNLLEPTRVSMPTENIVLEVAVLSTEGQIQDFKFPLGIKGAGSSIQLSANTVKQNSRNGLA KLVFIIYRSLGQFLSTENATIKLGADFIGRNSTIAVNSHVISVSINKESSRVYLTDPVLFTLPHIDPDNY FNANCSFWNYSERTMMGYWSTQGCKLVDTNKTRTTCACSHLTNFAILMAHREIAYKDGVHELLLTVITWV GIVISLVCLAICIFTFCFFRGLQSDRNTIHKNLCINLFIAEFIFLIGIDKTKYAIACPIFAGLLHFFFLA AFAWMCLEGVQLYLMLVEVFESEYSRKKYYYVAGYLFPATVVGVSAAIDYKSYGTEKACWLHVDNYFIWS FIGPVTFIILLNIIFLVITLCKMVKHSNTLKPDSSRLENIKSWVLGAFALLCLLGLTWSFGLLFINEETI VMAYLFTIFNAFQGVFIFIFHCALQKKVRKEYGKCFRHSYCCGGLPTESPHSSVKASTTRTSARYSSGTQ SRIRRMWNDTVRKQSESSFISGDINSTSTLNQGLTSHGLRAHLQDLYHLELLLGQIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001297705
ORF Size	3531 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297705.2
RefSeq Size	6127 bp
RefSeq ORF	3534 bp
Locus ID	23266
UniProt ID	O95490
Protein Families	Druggable Genome, GPCR, Transmembrane
MW	132.7 kDa
Gene Summary	This gene encodes a member of the latrophilin subfamily of G-protein coupled receptors. The encoded protein participates in the regulation of exocytosis. The proprotein is thought to be further cleaved within a cysteine-rich G-protein-coupled receptor proteolysis site into two chains that are non-covalently bound at the cell membrane. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

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