AP3B2 (NM_001278512) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DEE48; EIEE48; NAPTB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239964 representing NM_001278512 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCCGCCCCCGCCTACAGCGAAGACAAGGGCGGCTCCGCTGGCCCCGGGGAGCCCGAGTACGGCC ACGACCCCGCGAGCGGCGGCATCTTCTCCTCCGACTACAAGCGGCATGATGACCTGAAGGAGATGCTGGA CACCAACAAGGATTCTCTCAAGCTGGAGGCCATGAAGAGGATTGTGGCGATGATTGCCCGAGGAAAGAAT GCTTCAGACCTGTTTCCCGCGGTGGTGAAGAACGTGGCCTGTAAGAACATAGAGGTGAAGAAGCTTGTCT ATGTGTACCTGGTACGCTACGCTGAGGAGCAGCAAGACCTGGCCCTGCTGTCCATCTCCACCTTCCAACG TGGCCTAAAGGATCCCAACCAGCTGATTCGTGCCAGTGCCCTCCGTGTCCTCTCTAGCATCCGTGTGCCC ATCATAGTGCCCATCATGATGCTAGCTATCAAGGAAGCCGCCTCGGACATGTCACCCTATGTGCGGAAAA CAGCTGCCCACGCCATCCCTAAACTCTACAGTTTGGACTCTGACCAGAAGGATCAGCTGATAGAAGTCAT TGAGAAGCTTCTGGCTGACAAGACCACGCTGGTGGCGGGCAGTGTGGTGATGGCCTTTGAGGAGGTCTGC CCGGAGCGCATCGACCTGATTCACAAAAACTACCGGAAACTCTGTAACCTGCTGATCGACGTGGAGGAGT GGGGCCAGGTGGTCATCATCAGCATGCTCACCCGCTACGCCCGCACGCAGTTCCTGAGCCCCACCCAGAA CGAATCCCTACTAGAGGAGAACGCGGAAAAAGCCTTCTACGGCTCAGAGGAGGACGAGGCCAAGGGCGCG GGGTCTGAGGAGACGGCCGCCGCGGCCGCCCCCTCCCGAAAGCCCTATGTCATGGACCCCGACCACCGGC TGCTGCTGCGCAACACCAAACCCCTGCTGCAGAGCCGCAGCGCCGCGGTGGTGATGGCGGTGGCGCAGCT CTACTTCCACCTGGCGCCCAAGGCGGAAGTGGGCGTCATCGCCAAGGCGCTGGTGCGCCTGCTGCGCAGC CACAGTGAGGTGCAGTACGTTGTGCTCCAGAACGTGGCCACCATGTCCATCAAGCGCCGGGGTATGTTTG AGCCCTACCTGAAGAGCTTCTACATCAGGTCCACCGACCCCACCCAGATTAAGATCCTGAAGCTGGAAGT GCTGACCAACCTGGCCAATGAGACCAACATTCCTACTGTCCTACGGGAATTCCAGACCTATATTCGCAGC ATGGACAAGGACTTTGTGGCAGCCACAATCCAGGCCATTGGACGCTGTGCAACTAACATCGGCCGAGTCC GTGACACCTGCCTCAATGGCCTGGTGCAGCTGCTGTCCAACCGTGATGAGCTTGTGGTTGCAGAGTCAGT GGTCGTCATTAAGAAATTGCTACAGATGCAGCCAGCACAACATGGAGAGATCATCAAACACTTGGCAAAG CTTACAGACAACATCCAGGTGCCCATGGCCCGAGCCAGCATCCTGTGGCTCATCGGAGAGTACTGTGAGC ATGTCCCCAGGATTGCACCTGATGTCTTAAGAAAAATGGCCAAGTCATTCACAGCAGAGGAGGATATTGT CAAGCTGCAGGTCATCAACCTGGCAGCCAAGCTCTACCTGACCAACTCTAAACAGACCAAGCTGCTGACC CAGTATGTGCTGAGTCTGGCCAAATATGACCAGAACTATGATATTCGCGACCGGGCGCGCTTCACCCGGC AGCTCATCGTCCCTTCCGAGCAGGGTGGGGCCCTCAGCCGCCATGCCAAGAAGCTCTTCCTGGCACCCAA ACCAGCTCCAGTCTTGGAGTCATCCTTCAAAGACCGGGACCACTTCCAGCTGGGCTCACTGTCCCACCTG CTTAATGCCAAGGCCACAGGCTACCAGGAGCTCCCAGACTGGCCGGAGGAAGCCCCAGACCCATCTGTGC GCAACGTGGAGGAAGAAGATCTCTCCCTTATTGAGACTCACGTGGGCCTGTTGGGCGAATACACTGAGGT ACCTGAATGGACCAAGTGCTCAAATCGGGAGAAGAGAAAGGAGAAGGAAAAACCCTTCTACTCGGACTCT GAGGGGGAGTCAGGCCCCACGGAGTCCGCAGACAGTGACCCTGAGTCTGAGAGTGAATCGGACAGTAAGA GCAGCAGTGAGAGCGGCTCTGGGGAGTCCAGCAGTGAGTCCGACAATGAAGACCAGGATGAGGATGAGGA GAAAGGGAGAGGCAGTGAGAGTGAACAGAGTGAGGAGGATGGTAAGAGGAAGACAAAGAAGAAGGTGCCA GAGAGAAAAGGAGAAGCGTCATCCTCTGATGAGGGCAGCGATTCCAGCAGTAGCTCATCAGAGTCCGAGA TGACATCGGAGTCCGAGGAGGAGCAGTTAGAACCTGCCTCCTGGAGCAGGAAAACACCTCCCAGCAGCAA AAGTGCTCCTGCAACCAAGGAGATCTCCCTGCTTGATCTAGAGGATTTCACCCCTCCCAGTGTCCAGCCT GTGTCTCCCCCAGCAATTGTGTCTACCAGTCTGGCTGCTGACCTGGAGGGCCTGACACTCACAGACTCCA CCCTGGTACCGTCGCTTCTGAGTCCAGTATCGGGTGTTGGGCGGCAGGAGCTGCTGCACCGGGTAGCTGG CGAGGGGCTGGCTGTGGACTACACCTTCAGCCGCCAACCTTTCTCCGGGGATCCCCACATGGTGTCCGTG CACATCCACTTCTCCAACAGCTCTGATACCCCCATCAAGGGCCTGCATGTGGGCACTCCCAAACTGCCTG CTGGCATCAGCATCCAAGAATTTCCCGAAATTGAGTCCCTGGCACCTGGAGAATCTGCCACTGCTGTAAT GGGCATTAATTTCTGTGACTCAACCCAGGCAGCCAACTTCCAGCTGTGCACCCAGACCCGACAGTTCTAC GTCTCCATTCAGCCACCTGTTGGGGAGCTGATGGCCCCTGTGTTCATGAGTGAAAATGAGTTTAAGAAGG AACAGGGAAAGCTGATGGGCATGAATGAGATCACAGAGAAACTCATGCTGCCAGACACCTGTCGGAGTGA CCACATTGTGGTGCAGAAAGTGACTGCCACTGCCAACCTGGGTCGTGTTCCTTGTGGGACATCTGATGAG TACAGGTTTGCAGGGAGGACACTGACTGGTGGAAGCCTCGTTCTGCTGACCCTGGATGCCCGGCCAGCTG GAGCTGCCCAGCTGACTGTCAACAGCGAGAAAATGGTGATTGGCACCATGCTGGTAAAGGATGTGATACA GGCTCTGACCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239964 representing NM_001278512 Red=Cloning site Green=Tags(s) MSAAPAYSEDKGGSAGPGEPEYGHDPASGGIFSSDYKRHDDLKEMLDTNKDSLKLEAMKRIVAMIARGKN ASDLFPAVVKNVACKNIEVKKLVYVYLVRYAEEQQDLALLSISTFQRGLKDPNQLIRASALRVLSSIRVP IIVPIMMLAIKEAASDMSPYVRKTAAHAIPKLYSLDSDQKDQLIEVIEKLLADKTTLVAGSVVMAFEEVC PERIDLIHKNYRKLCNLLIDVEEWGQVVIISMLTRYARTQFLSPTQNESLLEENAEKAFYGSEEDEAKGA GSEETAAAAAPSRKPYVMDPDHRLLLRNTKPLLQSRSAAVVMAVAQLYFHLAPKAEVGVIAKALVRLLRS HSEVQYVVLQNVATMSIKRRGMFEPYLKSFYIRSTDPTQIKILKLEVLTNLANETNIPTVLREFQTYIRS MDKDFVAATIQAIGRCATNIGRVRDTCLNGLVQLLSNRDELVVAESVVVIKKLLQMQPAQHGEIIKHLAK LTDNIQVPMARASILWLIGEYCEHVPRIAPDVLRKMAKSFTAEEDIVKLQVINLAAKLYLTNSKQTKLLT QYVLSLAKYDQNYDIRDRARFTRQLIVPSEQGGALSRHAKKLFLAPKPAPVLESSFKDRDHFQLGSLSHL LNAKATGYQELPDWPEEAPDPSVRNVEEEDLSLIETHVGLLGEYTEVPEWTKCSNREKRKEKEKPFYSDS EGESGPTESADSDPESESESDSKSSSESGSGESSSESDNEDQDEDEEKGRGSESEQSEEDGKRKTKKKVP ERKGEASSSDEGSDSSSSSSESEMTSESEEEQLEPASWSRKTPPSSKSAPATKEISLLDLEDFTPPSVQP VSPPAIVSTSLAADLEGLTLTDSTLVPSLLSPVSGVGRQELLHRVAGEGLAVDYTFSRQPFSGDPHMVSV HIHFSNSSDTPIKGLHVGTPKLPAGISIQEFPEIESLAPGESATAVMGINFCDSTQAANFQLCTQTRQFY VSIQPPVGELMAPVFMSENEFKKEQGKLMGMNEITEKLMLPDTCRSDHIVVQKVTATANLGRVPCGTSDE YRFAGRTLTGGSLVLLTLDARPAGAAQLTVNSEKMVIGTMLVKDVIQALTQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278512
ORF Size	3303 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278512.2
RefSeq Size	3828 bp
RefSeq ORF	3306 bp
Locus ID	8120
UniProt ID	Q13367
Protein Pathways	Lysosome
MW	121.6 kDa
Gene Summary	Adaptor protein complex 3 (AP-3 complex) is a heterotrimeric protein complex involved in the formation of clathrin-coated synaptic vesicles. The protein encoded by this gene represents the beta subunit of the neuron-specific AP-3 complex and was first identified as the target antigen in human paraneoplastic neurologic disorders. The encoded subunit binds clathrin and is phosphorylated by a casein kinase-like protein, which mediates synaptic vesicle coat assembly. Defects in this gene are a cause of early-onset epileptic encephalopathy. [provided by RefSeq, Feb 2017]

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