IQGAP2 (NM_001285462) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239938 representing NM_001285462 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTGTTTTAAAGGAGTTGTAGCTGTAGGGTACATCAATGAAGCTATTGATGAAGGGAATCCTTTGA GGACTTTAGAAACTTTGCTCCTACCTACTGCGAATATTAGTGATGTGGACCCAGCCCATGCCCAGCACTA CCAGGATGTTTTATACCATGCTAAATCACAGAAACTCGGAGACTCTGAGAGTGTTTCCAAAGTGCTTTGG CTGGATGAGATACAGCAAGCCGTCGATGATGCCAACGTGGACGAGGACAGAGCAAAACAATGGGTTACTC TGGTGGTTGATGTTAATCAGTGTTTGGAAGGAAAAAAATCAAGTGATATTTTGTCTGTATTGAAGTCTTC CACTTCTAATGCAAATGACATAATCCCGGAGTGTGCTGACAAATACTATGATGCCCTTGTGAAGGCAAAA GAGCTCAAATCTGAAAGAGTGTCTAGTGACGGTTCATGGCTCAAACTCAACCTGCACAAAAAATATGACT ACTATTACAACACTGATTCAAAAGAGAGTTCCTGGGTCACACCTGAATCATGCTTGTATAAAGAATCATG GCTCACAGGAAAAGAAATCGAGGCTTTTTGGAAAGGATATAAACAACGGAAGGAGTATATGCACAGGCGG CAAACGTTCATTGATAATACTGATTCTATTGTGAAGATTCAGTCCTGGTTCCGAATGGCAACTGCAAGAA AGAGCTATCTTTCAAGACTACAGTATTTCAGAGATCATAATAATGAAATTGTGAAAATACAGTCACTGTT GAGAGCGAACAAAGCTAGAGATGACTACAAAACATTGGTTGGCTCTGAAAACCCACCATTAACAGTAATT CGCAAATTTGTATACCTGCTGGACCAAAGTGATTTGGATTTCCAGGAGGAACTAGAGGTTGCACGATTAA GGGAAGAAGTAGTGACCAAGATCAGGGCCAATCAACAGCTGGAAAAAGACCTGAACCTGATGGACATCAA GATTGGACTGCTGGTGAAGAACAGGATCACACTAGAGGATGTAATTTCACACAGTAAAAAGCTGAACAAG AAAAAAGGAGGAGAAATGGAAATACTGAATAACACCGACAACCAAGGAATAAAAAGTTTGAGTAAGGAGA GGAGAAAAACACTAGAAACATATCAGCAGCTGTTTTACCTTTTACAGACCAACCCTTTATACTTGGCTAA GCTGATTTTCCAGATGCCACAGAACAAGTCCACTAAATTTATGGATACTGTTATTTTCACACTATATAAT TATGCCTCTAATCAGCGAGAAGAATATCTACTTCTCAAGCTTTTTAAAACTGCTCTGGAGGAAGAAATAA AATCAAAAGTGGACCAGGTACAGGACATAGTTACTGGTAACCCTACAGTCATCAAGATGGTCGTCAGCTT CAATAGAGGTGCCCGGGGACAGAACACCCTGCGCCAACTCCTGGCTCCAGTGGTAAAAGAGATCATCGAC GACAAGTCGCTGATTATCAACACAAACCCTGTAGAGGTGTACAAGGCTTGGGTGAACCAACTAGAAACAC AGACTGGAGAGGCCAGCAAGTTGCCTTATGATGTGACCACAGAACAAGCTCTAACATACCCAGAAGTGAA AAATAAACTGGAGGCTTCCATTGAGAACCTGAGAAGGGTCACCGACAAAGTCCTGAATTCTATCATTTCT TCCCTTGATCTACTGCCTTATGGATTGAGGTATATAGCCAAAGTACTGAAGAATTCGATCCATGAGAAAT TCCCCGATGCAACAGAAGATGAGCTATTAAAGATTGTTGGAAACCTCCTGTACTATCGGTACATGAATCC AGCCATTGTAGCTCCAGATGGCTTTGATATCATCGACATGACAGCTGGAGGTCAGATAAATTCTGACCAA AGGAGAAACTTAGGATCAGTGGCCAAGGTTCTTCAGCACGCAGCCTCCAACAAGCTGTTTGAAGGAGAAA ATGAGCATCTCTCATCTATGAACAATTATTTATCAGAGACGTATCAGGAATTCAGGAAATATTTCAAAGA AGCATGTAATGTCCCTGAGCCAGAAGAGAAGTTTAATATGGACAAATACACAGACCTGGTGACAGTCAGC AAACCAGTCATTTATATTTCAATTGAAGAAATCATCAGCACACACTCACTCCTGTTGGAACACCAGGATG CAATTGCCCCTGAGAAAAATGACTTACTGAGTGAATTGCTGGGGTCGCTGGGAGAGGTGCCAACCGTGGA ATCTTTTCTTGGGGAAGGAGCAGTTGACCCCAATGACCCTAACAAGGCAAATACACTAAGTCAGCTTTCA AAGACCGAGATTTCTCTTGTCTTGACAAGCAAATATGACATAGAGGACGGTGAAGCTATAGATAGCCGAA GCCTCATGATAAAGACCAAGAAGCTGATAATTGATGTGATCCGGAACCAGCCAGGGAACACATTGACAGA AATCTTAGAGACACCAGCAACTGCGCAACAGGAGGTAGACCATGCCACGGACATGGTGAGCCGTGCAATG ATAGATTCCAGGACTCCAGAAGAAATGAAGCATAGCCAATCTATGATTGAAGATGCACAGCTGCCTCTTG AGCAGAAGAAGAGGAAAATCCAGAGGAATCTTCGGACGTTGGAACAGACTGGACACGTGTCATCCGAAAA TAAATACCAAGACATTCTCAATGAGATTGCCAAGGATATTCGAAATCAAAGAATCTATCGTAAGCTTCGA AAAGCTGAATTGGCAAAACTTCAGCAGACCCTGAATGCACTTAACAAGAAGGCAGCATTTTATGAAGAGC AAATCAATTATTATGACACCTACATAAAGACTTGTTTAGACAACTTAAAAAGAAAAAATACTCGGAGATC AATTAAACTAGATGGAAAAGGAGAACCCAAAGGGGCGAAGAGAGCGAAGCCAGTGAAGTACACTGCAGCA AAGCTGCATGAGAAAGGTGTCCTGCTAGATATAGATGATCTTCAAACAAACCAGTTTAAGAATGTTACAT TTGATATCATAGCTACTGAAGATGTAGGCATTTTCGATGTAAGATCAAAATTCCTTGGTGTTGAGATGGA AAAGGTGCAACTCAATATTCAGGATTTACTTCAGATGCAATATGAAGGAGTAGCTGTAATGAAAATGTTT GATAAGGTTAAAGTGAATGTAAACCTTCTCATATACCTGCTGAACAAGAAGTTCTATGGAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239938 representing NM_001285462 Red=Cloning site Green=Tags(s) MGCFKGVVAVGYINEAIDEGNPLRTLETLLLPTANISDVDPAHAQHYQDVLYHAKSQKLGDSESVSKVLW LDEIQQAVDDANVDEDRAKQWVTLVVDVNQCLEGKKSSDILSVLKSSTSNANDIIPECADKYYDALVKAK ELKSERVSSDGSWLKLNLHKKYDYYYNTDSKESSWVTPESCLYKESWLTGKEIEAFWKGYKQRKEYMHRR QTFIDNTDSIVKIQSWFRMATARKSYLSRLQYFRDHNNEIVKIQSLLRANKARDDYKTLVGSENPPLTVI RKFVYLLDQSDLDFQEELEVARLREEVVTKIRANQQLEKDLNLMDIKIGLLVKNRITLEDVISHSKKLNK KKGGEMEILNNTDNQGIKSLSKERRKTLETYQQLFYLLQTNPLYLAKLIFQMPQNKSTKFMDTVIFTLYN YASNQREEYLLLKLFKTALEEEIKSKVDQVQDIVTGNPTVIKMVVSFNRGARGQNTLRQLLAPVVKEIID DKSLIINTNPVEVYKAWVNQLETQTGEASKLPYDVTTEQALTYPEVKNKLEASIENLRRVTDKVLNSIIS SLDLLPYGLRYIAKVLKNSIHEKFPDATEDELLKIVGNLLYYRYMNPAIVAPDGFDIIDMTAGGQINSDQ RRNLGSVAKVLQHAASNKLFEGENEHLSSMNNYLSETYQEFRKYFKEACNVPEPEEKFNMDKYTDLVTVS KPVIYISIEEIISTHSLLLEHQDAIAPEKNDLLSELLGSLGEVPTVESFLGEGAVDPNDPNKANTLSQLS KTEISLVLTSKYDIEDGEAIDSRSLMIKTKKLIIDVIRNQPGNTLTEILETPATAQQEVDHATDMVSRAM IDSRTPEEMKHSQSMIEDAQLPLEQKKRKIQRNLRTLEQTGHVSSENKYQDILNEIAKDIRNQRIYRKLR KAELAKLQQTLNALNKKAAFYEEQINYYDTYIKTCLDNLKRKNTRRSIKLDGKGEPKGAKRAKPVKYTAA KLHEKGVLLDIDDLQTNQFKNVTFDIIATEDVGIFDVRSKFLGVEMEKVQLNIQDLLQMQYEGVAVMKMF DKVKVNVNLLIYLLNKKFYGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001285462
ORF Size	3213 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285462.1, NP_001272391.1
RefSeq Size	4171 bp
RefSeq ORF	3216 bp
Locus ID	10788
UniProt ID	Q13576
Protein Families	Druggable Genome
Protein Pathways	Regulation of actin cytoskeleton
MW	123.3 kDa
Gene Summary	This gene encodes a member of the IQGAP family. The encoded protein contains three IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. This protein interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. It also acts as a tumor suppressor and has been found to play a role in regulating innate antiviral responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2017]

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